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Discover how researchers are using Oxford Nanopore sequencing to generate new insights across diverse applications.

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Discover nanopore sequencing

  • What it can do
  • How it’s being used
  • How to get started

Latest protocols

native-barcoding-kit 3Workflow

Pharmacogenomic (PGx) sequencing workflow with adaptive sampling (blood, cells, saliva)

This protocol provides an end-to-end workflow for the analysis of pharmacogenomic (PGx) genes using adaptive sampling, and the Oxford Nanopore platform.

Long read de novo sequencing from blood and cells using SQK-ULK114 – resolve method

This protocol describes a workflow for long read de novo sequencing of the human genome using the Oxford Nanopore PromethION platform to generate near-T2T de novo assemblies from long read data.

Long read de novo sequencing from blood using SQK-LSK114 – scale method

This protocol describes a workflow for long read de novo sequencing of the human genome using the Oxford Nanopore PromethION platform to generate near-T2T de novo assemblies from long read data.
View all end-to-end workflows
SQK-RBK110.96 gDNA workflow v1Workflow

Rapid sequencing DNA V14 - barcoding (SQK-RBK114.24 or SQK-RBK114.96)

Learn how to perform rapid genomic DNA barcoding using the Rapid Barcoding Kit V14 (SQK‑RBK114.24 / SQK‑RBK114.96). This fast, high‑yield library preparation workflow enables multiplexing of up to 96 gDNA samples with ~60‑minute prep time and compatibility with R10.4.1 flow cells.

Rapid Sequencing Kit V14 - gDNA (SQK-RAD114)

The fastest and simplest protocol for genomic DNA sequencing. For Research Use Only
ONT Logo wheel blackWorkflow

Microbial amplicon barcoding for 16S and ITS – automated ElysION MinION (SQK-MAB114.24)

This is an automated microbial amplicon barcoding method using the ElysION™ device with a MinION™ setup, outlining library preparation and sequencing.
View all library prep protocols

Webinars and events

plasmidWebinar

Unlocking high-resolution, strain-resolved microbiome analyses | ASM Microbe 26

During this video we learn how full length Oxford Nanopore sequencing is unlocking high-resolution microbiome analyses

View recent webinars
mRNAEvent

Oxford Nanopore at AMP 2026

Oxford Nanopore Technologies are exhibiting and presenting at AMP 2026 in November.

View upcoming events

White papers

Preview image for the plant, animal, and environmental sequencing white paper, 'Genomics for a changing planet'.White paper

Genomics for a changing planet: sequencing the living world

Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.

Clinical research white paper coverWhite paper

New approaches for human and clinical research

Explore the advantages of Oxford Nanopore sequencing to detect genomic and epigenomic variants in human and clinical research.

Case studies

Targeted sequencing in cancerCase study

Faster analysis of cancer-associated variants using a unique targeted nanopore sequencing approach

In this case study, discover the benefits of adaptive sampling and how it has been applied to cancer research.

CancerCase study

Accurate identification of cancer-predisposing deep intronic variants in tumour-suppressor genes with Oxford Nanopore sequencing

Find out how targeted Oxford Nanopore sequencing, via adaptive sampling, can be used to systemically evaluate deep intronic variants associated with hereditary cancer risk.

Getting started guides

Two-page image of the biopharma getting started guideGetting started guide

A guide to biopharma solutions from Oxford Nanopore — from discovery to development

This guide introduces Oxford Nanopore sequencing solutions for biopharma that can be applied from biomarker discovery through to final product quality control (QC) testing.

Preview image for the bulk transcriptomics getting started guideGetting started guide

A guide to transcriptomics with Oxford Nanopore

This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.

Workflow overviews

Image showing a preview of the PGx with adaptive sampling workflowWorkflow overview

Real-time enrichment of a comprehensive pharmacogenomic panel with Oxford Nanopore sequencing

This end-to-end workflow introduces how to enrich a comprehensive panel of PGx targets with real-time targeted Oxford Nanopore sequencing.

Workflow: human variant calling — 2 pagesWorkflow overview

Comprehensive human genomic variant and methylation analysis with long Oxford Nanopore reads

This end-to-end workflow provides a scalable method to identify previously hidden and potentially pathogenic variants.

Brochures

Flyer: Hereditary Cancer Panel — 2 pagesBrochure

Hereditary Cancer Panel: targeted sequencing via adaptive sampling

In this flyer, discover how to utilise the Oxford Nanopore Hereditary Cancer Panel to investigate 258 key genes associated with hereditary cancer risk.

Preview of the methylation flyerBrochure

Go with the gold standard in methylation sequencing

In this flyer, find out how Oxford Nanopore provides gold-standard methylation sequencing and comprehensive variant calling in a single run — without chemical conversion.

Image of a PromethION 24 in the lab next to two scientists

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