Assembly
Getting started guides
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A guide to microbial sequencing with Oxford Nanopore
A guide to get started with sequencing microbial samples with Oxford Nanopore.
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A guide to human genomics with Oxford Nanopore
This guide introduces how to sequence human genomes with Oxford Nanopore, from the construction of new, highly complete reference assemblies to the comprehensive identification of variants.
Workflow overviews
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Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing
This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.
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Rapid and complete plasmid characterisation with Oxford Nanopore sequencing
This end-to-end workflow is a rapid method for complete, high-quality whole-plasmid characterisation.
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Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches
This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.
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Assembling high-quality plant genomes with Oxford Nanopore
This end-to-end workflow introduces how to generate a high-quality plant genome assembly from a leaf sample.
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Accelerating clinical research with Oxford Nanopore large cohort sequencing
This end-to-end workflow provides a scalable solution for genome-wide analysis of genomic and epigenomic variants across a large cohort of human research samples with Oxford Nanopore sequencing.
Most viewed
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cDNA-PCR Sequencing Kits
In this flyer, discover how you can sequence full-length transcripts and annotate the transcriptome with confidence.
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Oxford Nanopore sequencing provides superior metagenome-assembled genome recovery and strain-level resolution from a complex microbiome
In this application note, we demonstrate the
capabilities of Oxford Nanopore metagenomics by
sequencing and analysing the well-characterised
ZymoBIOMICS Fecal Reference.
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Oxford Nanopore whole-genome sequencing of foodborne pathogens
In this application note, we demonstrate the suitability of Oxford Nanopore sequencing as a standalone platform for foodborne pathogen whole-genome sequencing.
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Oxford Nanopore sequencing solutions for microbiology and infectious disease research
Discover the Oxford Nanopore sequencing techniques that can achieve comprehensive microbial genome characterisation.
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EPI2ME: data analysis for all levels of expertise
Discover EPI2ME and how it provides data analysis for all levels of expertise
Featured resources
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Accelerating cancer research through comprehensive genomic analysis
Unlock previously hidden variation with nanopore sequencing and characterise cancer at unprecedented resolution.
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High-quality bacterial genomes without the complexity
In this case study, researchers show that near-complete bacterial genomes can be assembled using nanopore sequencing alone.
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From full-length 16S to high-resolution metagenomics: how do I choose the right microbial community workflow for my experiment?
In this masterclass, find the right microbial community sequencing workflow to meet your experimental goals. We’ll cover microbial identification from full-length 16S/ITS sequencing through to comprehensive metagenomic assembly. In this masterclass, discover: • How Oxford Nanopore sequencing deliver
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From speed to scale: how do I choose the right human whole-genome sequencing workflow for my experiment?
In this masterclass, find the right human whole-genome sequencing workflow to meet your experimental goals. We’ll cover whole-genome analysis in just 24 hours through to scaling sequencing for large cohort research. In this masterclass, discover: • How Oxford Nanopore sequencing is advancing human g
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How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
Protocols
Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)
Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)
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Plasmid sequencing from DNA using SQK-RBK114 (.24 or .96)
This protocol describes how to carry out rapid barcoding of plasmid DNA using the Rapid Barcoding Kit 24 or 96 V14 (SQK-RBK114.24 or SQK-RBK114.96) to sequence up to 96 plasmid samples.
Analysis workflows
Latest research
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Comprehensive resolution of challenging genomic variants with Oxford Nanopore de novo genome assemblies
Find out how to generate telomere-to-telomere genome assemblies using long and ultra-long nanopore reads for platinum-standard references.
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Telomere-to-telomere assembly using HERRO-corrected simplex nanopore reads
Publication: Telomere-to-telomere assembly using HERRO-corrected simplex nanopore reads
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High-resolution metagenome assembly for modern long reads with myloasm
Publication: High-resolution metagenome assembly for modern long reads with myloasm
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Masterclass: How to sequence full-length plasmids in your own lab | LC25
Discover how to perform on-demand sequencing of full-length plasmids securely in your own lab, using a complete sample-to-answer workflow with Oxford Nanopore technology.
In this masterclass, you will learn:
How Oxford Nanopore sequencing delivers accurate, flexible, and secure plasmid c
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Masterclass: How to call variants and methylation across the human genome | LC25
Explore Oxford Nanopore’s ultra-rich multiomic workflows for comprehensive human variation analysis — including SNVs, structural variants, methylation, STRs, CNVs, and phasing.
In this masterclass, you will learn:
How to obtain multiomic data from a single sequencing run
The sample-t
