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Targeted sequencing

Getting started guides

Two-page image of the biopharma getting started guideGetting started guide

A guide to biopharma solutions from Oxford Nanopore — from discovery to development

This guide introduces Oxford Nanopore sequencing solutions for biopharma that can be applied from biomarker discovery through to final product quality control (QC) testing.
Two-page image of the targeted sequencing getting started guideGetting started guide

A guide to targeted sequencing with Oxford Nanopore

This guide provides an introduction to performing targeted nanopore sequencing.
Two-page overview of the human genomics getting started guideGetting started guide

A guide to human genomics with Oxford Nanopore

This guide introduces how to sequence human genomes with Oxford Nanopore, from the construction of new, highly complete reference assemblies to the comprehensive identification of variants.
Two-page image of the methylation getting started guideGetting started guide

A guide to investigating methylation in the human genome

This guide provides an introduction to the direct sequencing of DNA methylation in human genomes.

Workflow overviews

Image showing a preview of the PGx with adaptive sampling workflowWorkflow overview

Real-time enrichment of a comprehensive pharmacogenomic panel with Oxford Nanopore sequencing

This end-to-end workflow introduces how to enrich a comprehensive panel of PGx targets with real-time targeted Oxford Nanopore sequencing.
Two-page image of the Hereditary Cancer Panel workflowWorkflow overview

Comprehensive characterisation of cancer predisposition genes using the Hereditary Cancer Panel

Discover the Hereditary Cancer Panel workflow and streamline your precision oncology research.
Microbiology MetagenomicsWorkflow overview

Performing accurate species-level bacterial identification with nanopore sequencing

This end-to-end workflow provides a rapid solution for species-level bacterial identification.
Preview of the microbial amplicon barcoding workflow.Workflow overview

Streamlined identification of bacteria, archaea, and fungi with Oxford Nanopore microbial amplicon barcoding

This end-to-end workflow introduces how to sequence the full length 16S gene and ITS region with Oxford Nanopore technology.
Microbiology stillWorkflow overview

Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches

This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.

Most viewed

Flyer: Hereditary Cancer Panel — 2 pagesBrochure

Hereditary Cancer Panel: targeted sequencing via adaptive sampling

In this flyer, discover how to utilise the Oxford Nanopore Hereditary Cancer Panel to investigate 258 key genes associated with hereditary cancer risk.
Application note: Hereditary Cancer Panel — 2 pagesApplication note

Comprehensive genomic and epigenomic profiling with the Oxford Nanopore Hereditary Cancer Panel

In this application note, we present the design and performance of the Oxford Nanopore Hereditary Cancer Panel targeting 258 key genes implicated in cancer predisposition.
Preview of the methylation flyerBrochure

Go with the gold standard in methylation sequencing

In this flyer, find out how Oxford Nanopore provides gold-standard methylation sequencing and comprehensive variant calling in a single run — without chemical conversion.
Brochure: GridION Q — 2 pagesBrochure

GridION Q: Oxford Nanopore sequencing designed for use in regulated and applied environments

Discover GridION Q, the sequencing solution designed for use in regulated and applied environments from Oxford Nanopore Technologies.
Preview for the microbial sequencing brochureBrochure

Oxford Nanopore sequencing solutions for microbiology and infectious disease research

In this brochure, discover Oxford Nanopore sequencing techniques that can reveal multidimensional insights for your microbial research applications.

Preview image for the plant, animal, and environmental sequencing white paper, 'Genomics for a changing planet'.White paper

Genomics for a changing planet: sequencing the living world

Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
White paper: cancer research — 2 pagesWhite paper

Accelerating cancer research through comprehensive genomic analysis

Unlock previously hidden variation with nanopore sequencing and characterise cancer at unprecedented resolution.
Real-time targeted sequencingEvent video

How do I perform real-time targeted Oxford Nanopore sequencing?

What if targeted sequencing took place during sequencing itself, without the need for special library prep, PCR, or probes? In this demo, discover Adaptive Sampling: a unique, real-time targeted enrichment/depletion method that achieves just that.
Targeted sequencing in cancerCase study

Faster analysis of cancer-associated variants using a unique targeted nanopore sequencing approach

In this case study, discover the benefits of adaptive sampling and how it has been applied to cancer research.
Which_library_prep_workflow_is_right_for_my_experimentEvent video

Which library prep workflow is right for my experiment?

Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow.

Protocols

Plasmid RBK114v2Workflow

Plasmid sequencing from DNA using SQK-RBK114 (.24 or .96)

This protocol describes how to carry out rapid barcoding of plasmid DNA using the Rapid Barcoding Kit 24 or 96 V14 (SQK-RBK114.24 or SQK-RBK114.96) to sequence up to 96 plasmid samples.

Amplicon sequencing from DNA using SQK-RBK114 (.24 or .96)

This is the fastest and simplest protocol to sequence amplicon DNA. For Research Use Only Document version: RAA_9198_v114_revM_17Oct2025

Analysis workflows

wf-tb-amr

wf-tb-amr is a workflow for determining the antibiotic resistance of Mycobacterium tuberculosis targeted sequencing samples.

wf-mpx

This workflow provides a simple way to analyse mpox sequencing data; taking raw Oxford Nanopore Technologies reads and creating a draft consensus and assembly.

wf-flu

This analysis workflow can be used with Oxford Nanopore Technologies sequencing data from amplified segments of the Influenza Type A and Type B genomes, to determine the most likely strain of Influenza to which the sequenced sample belongs.

wf-cas9

The ONT Cas9 sequencing kit allows the enrichment of genomic regions of interest by amplifying target regions from adapters ligated to Cas9 cleavage sites. The purpose of this workflow is to assess the effectiveness of such Cas9 enrichment, but it can be applied to other enrichment approaches.

wf-bacterial-genomes

This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.

Latest research

Graphical image of a DNA molecule passing through a nanopore, generating a characteristic and measurable disruption to the electrical signalPublication

Nanopore sequencing combined with Adaptive Sampling and NanoExpansion enables accurate characterisation of repeat expansion disorders

Authors: Luca Morandi, Francesco Casadei, Sara De Fanti, Luigi Bonan, Francesco Ravaioli, Flavia Palombo, Alessia Fiorentino, Silvia de Pasqua, Veria Vacchiano, Patrizia Avoni, Giovanni Rizzo, Sabina Capellari, Edoardo Ruggeri, Vincenzo Donadio, Gastone Castellani, Maria Giulia Bacali
plasmidWebinar

Strengthening MRSA genomic surveillance in the Pacific

Discover how strengthening local sequencing and bioinformatics capacity can support sustainable pathogen surveillance and informed public health responses across the Pacific.
Oxford Nanopore methylationWebinar

Richer insights with adaptive sampling: targeted panels without probes for cancer predisposition, tumor profiling and beyond

Learn about Oxford Nanopore's off-the-shelf solutions for creating simple, rapid custom panels providing data-rich scalable insights into cancer biology.
Joy GoffenaResearch presentation

Variant phasing for antisense oligonucleotide design using adaptive sampling | LC 25

Joy Goffena is a Research Scientist in Dr Danny Miller’s lab at the University of Washington. She specializes in applying Oxford Nanopore Technologies sequencing to advance genetic research and clinical diagnostics. Her work focuses on identifying causal genetic variants...
Sebastian LunkeResearch presentation

Long-read sequencing and adaptive sampling solve complex diagnostic conundrums | LC 25

Prof. Sebastian Lunke is the Head of Division of Genetics and Genomics at the Victorian Clinical Genetics Services. His team oversees the development and delivery of clinically accredited genetics and genomics laboratories in Australia, with a strong focus on translating...

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