Epigenetics
Getting started guides
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A guide to targeted sequencing with Oxford Nanopore
This guide provides an introduction to performing targeted nanopore sequencing.
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A guide to human genomics with Oxford Nanopore
This guide introduces how to sequence human genomes with Oxford Nanopore, from the construction of new, highly complete reference assemblies to the comprehensive identification of variants.
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A guide to investigating methylation in the human genome
This guide provides an introduction to the direct sequencing of DNA methylation in human genomes.
Workflow overviews
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Detecting isoforms and modifications with PCR-free, direct RNA nanopore sequencing
This end-to-end workflow provides a simple method to characterise RNA modifications from a human blood research sample using direct RNA sequencing.
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Characterising genomic and epigenomic variation between tumour-normal research samples using long nanopore sequencing reads
This end-to-end workflow overview provides a simple solution for detecting a wide range of tumour-specific variation in a single sequencing assay.
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Rapid identification of pathogenic variants and methylation with whole-genome Oxford Nanopore sequencing
This end-to-end workflow introduces how to perform rapid whole-genome human variant and methylation calling from a blood research sample using high-output DNA sequencing on PromethION 24.
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Assembling high-quality plant genomes with Oxford Nanopore
This end-to-end workflow introduces how to generate a high-quality plant genome assembly from a leaf sample.
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Accelerating clinical research with Oxford Nanopore large cohort sequencing
This end-to-end workflow provides a scalable solution for genome-wide analysis of genomic and epigenomic variants across a large cohort of human research samples with Oxford Nanopore sequencing.
Most viewed
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Multiomic nanopore sequencing: revolutionising human disease research
Find out how multiomic nanopore sequencing can accelerate your disease research using a single platform.
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Accelerate your epigenetics research with Oxford Nanopore sequencing
In this flyer, find out how Oxford Nanopore sequencing uncovers the complete methylome and provides highly accurate and comprehensive base modification data for analysis.
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Oxford Nanopore 24-hour whole-genome sequencing for paediatric rare disease research
Discover how the Oxford Nanopore rapid whole-genome sequencing workflow is delivering fast, actionable results in 24 hours from sample collection.
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EPI2ME: data analysis for all levels of expertise
Discover EPI2ME and how it provides data analysis for all levels of expertise
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Comprehensive genomic and epigenomic profiling with the Oxford Nanopore Hereditary Cancer Panel
In this application note, we present the design and performance of the Oxford Nanopore Hereditary Cancer Panel targeting 258 key genes implicated in cancer predisposition.
Featured resources
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Cell-free DNA sequencing and methylation detection — promising potential for non-invasive cancer monitoring
In this case study, find out how researchers are using nanopore technology to analyse cfDNA for cancer monitoring.
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Unlocking RNA biology with full-length reads
Discover how nanopore sequencing uniquely captures full-length transcripts for isoform-level transcriptome characterisation.
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Large-cohort cDNA sequencing advances multiomic insights into neurodegenerative disease
In this case study, discover how Kimberley Billingsley and her colleagues at the National Institutes of Health are using Oxford Nanopore large-cohort cDNA sequencing to research the human brain transcriptome, enhancing understanding of the drivers of neurodegenerative diseases.
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From speed to scale: how do I choose the right human whole-genome sequencing workflow for my experiment?
In this masterclass, find the right human whole-genome sequencing workflow to meet your experimental goals. We’ll cover whole-genome analysis in just 24 hours through to scaling sequencing for large cohort research. In this masterclass, discover: • How Oxford Nanopore sequencing is advancing human g
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How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
Protocols
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Human cfDNA singleplex sequencing from blood using SQK-LSK114
This protocol describes how to carry out preparation and sequencing of a human cfDNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). Typically, we obtain ~50 Gb of aligned data (15x coverage) for human cfDNA samples processed with this protocol.
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Human cfDNA multiplex sequencing from blood using SQK-NBD114.24
This protocol describes how to carry out preparation and sequencing of 12 human cell-free DNA (cfDNA) samples using the Native Barcoding Kit 24 V14 (SQK-NBD114.24). Typically, we obtain ~3 Gb of aligned data (1x coverage) for each of the 12 human cfDNA samples processed with this protocol.
Reduced representation methylation sequencing (RRMS) from cells using SQK-LSK114
For Research Use Only.
Reduced representation methylation multiplex sequencing (RRMS) from cells using SQK-NBD114.24
For Research Use Only.
Analysis workflows
wf-metagenomics
This workflow can be used for the taxonomic classification of metagenomic sequencing data.
Latest research
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Targeted long-read sequencing enables comprehensive analysis of the genetic and epigenetic landscape of inherited myopathies
Authors: Dennis Yeow, Andre L. M. Reis, Igor Stevanovski, et al.
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Beyond bisulfite sequencing: resolving 5-hmC with nanopore sequencing unmasks the true-5mC methylation entropy landscape
Authors: Uri Bertocchi, Eyal Katz, Jonathan Jeffet, Assaf Grunwald, Neiv Gabay, Jasline Deek, Sujal Verma, Amit Shwartz, Gali Umschweif–Nevo, Bernard Lerer, Yael Roichman, Yuval Ebenstein
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Hierarchical classification of haematologic malignancies using epigenetic and genetic information
Authors: Maximilian Schönung, Melissa Türe, Panna Lajer, et al.
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Long-read sequencing maps transposable element variation and its regulatory and epigenetic effects in the human brain
Authors: Alexis Ayuketah, Melissa Meredith, Cristian Groza, et al.
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Structural and epigenetic characterisation of D4Z4 arrays in FSHD using Oxford Nanopore multiomic sequencing
Find out how Oxford Nanopore sequencing reveals genetic and epigenetic variation to characterise FSHD.
