A guide to transcriptomics with Oxford Nanopore
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- Published on: July 3 2026
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For insights into complex biological mechanisms, a deep understanding of RNA diversity within transcriptomes is key. Oxford Nanopore sequencing delivers high outputs of reads that span full-length transcripts, providing unambiguous isoform detection for isoform expression analysis, as well as robust gene expression profiles. We offer streamline, end-to-end workflows for bulk transcriptome analysis, through cDNA sequencing or direct RNA sequencing — the only available technique that directly reads single, native RNA molecules. This guide introduces cDNA and direct RNA nanopore sequencing, for ultra-rich transcriptomic data without compromise.
In this getting started guide, you will:
Find out about the benefits of nanopore sequencing for bulk transcriptomics, and how it reveals isoform-level information missed by legacy short-read technologies
Discover how to go from sample to answer with streamlined, end-to-end workflows for cDNA and direct RNA nanopore sequencing
Find out about the range of PromethION sequencing devices
Learn how nanopore transcriptomics data can be easily analysed using the EPI2ME data analysis workflow wf-transcriptomes
Read real-world case studies describing how researchers are utilising cDNA and direct RNA nanopore sequencing to reveal hidden diversity in health and disease
