Resources: Documentation

This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). It is recommended that a Lambda control experiment is completed first to become familiar with the technology.

This protocol: Uses genomic DNA Has a library preparation time ~65 minutes Has an optional fragmentation step Requires no PCR Is suitable for processing multiple samples simultaneously, or automated library preparation Compatible with R10.4.1 flow cells For Research Use Only

Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)

Telomere-to-telomere sequencing (T2T) from blood and cells using SQK-APK114, SQK-LSK114, and SQK-ULK114

This is a 10X Genomics Visium Spatial transcriptomics method.

This is a single-cell transcriptomics method.

This protocol is for the extraction and sequencing of ultra-high molecular weight (uHMW) genomic DNA.

Loading multiple PromethION Flow Cells

For Research Use Only.

This protocol describes how to carry out rapid barcoding of plasmid DNA using the Rapid Barcoding Kit 24 or 96 V14 (SQK-RBK114.24 or SQK-RBK114.96) to sequence up to 96 plasmid samples.

This is the fastest and simplest protocol to sequence amplicon DNA. For Research Use Only Document version: RAA_9198_v114_revM_17Oct2025

The fastest and simplest protocol for genomic DNA sequencing. For Research Use Only

This is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. For Research Use Only

Learn how to perform rapid genomic DNA barcoding using the Rapid Barcoding Kit V14 (SQK‑RBK114.24 / SQK‑RBK114.96). This fast, high‑yield library preparation workflow enables multiplexing of up to 96 gDNA samples with ~60‑minute prep time and compatibility with R10.4.1 flow cells.

This protocol: Uses genomic DNA Has a low input requirement Has a method involving tagmentation, barcoding and PCR amplification Allows multiplexing of 1–24 samples Is compatible with R10.4.1 flow cells For Research Use Only

Rapid sequencing DNA - 16S Barcoding Kit 24 V14 (SQK-16S114.24)

This protocol describes the step-by-step instructions to complete a Lambda Control experiment using the Rapid Sequencing Kit V14 (SQK-RAD114) on a MinION Flow Cell. It allows you to become familiar with nanopore sequencing before preparing your own library.

This is a legacy method. Please refer to our new equivalent method: SARS-CoV-2 virus PCR tiling and sequencing from RNA using SQK-RBK114.96 For Research Use Only This is an Early Access product. Document version: MRT_9186_v114_revL_8Dec2025

cDNA-PCR Sequencing V14 (SQK-PCS114)

cDNA-PCR Sequencing V14 - Barcoding (SQK-PCB114.24)

Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96)

This protocol: Uses genomic DNA Has a hands-on setup time ~30 minutes Has a automated run time ~3 hours 40 minutes Has the ability to process 6–96 samples for multiplex library loading Is compatible with R10.4.1 Flow Cells For Research Use Only

Step-by-step protocol for microbial 16S and ITS amplicon sequencing using the Oxford Nanopore Microbial Barcoding Kit.

Learn how to sequence full‑length 5′ cDNA transcripts from 10x Genomics single‑cell assays using the Oxford Nanopore Ligation Sequencing Kit V14 (SQK‑LSK114). This high‑output method enables detailed isoform analysis, alternative splicing detection, SNP resolution, and single‑cell subtype identifica

This protocol describes how to carry out preparation and sequencing of a human cfDNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). Typically, we obtain ~50 Gb of aligned data (15x coverage) for human cfDNA samples processed with this protocol.

This protocol describes how to carry out preparation and sequencing of 12 human cell-free DNA (cfDNA) samples using the Native Barcoding Kit 24 V14 (SQK-NBD114.24). Typically, we obtain ~3 Gb of aligned data (1x coverage) for each of the 12 human cfDNA samples processed with this protocol.

Ligation sequencing V14 - Direct cDNA sequencing (SQK-LSK114)

This document describes the sequencing of the influenza virus. For Research Use Only

This protocol: Uses genomic DNA Has very low input requirements (e.g. single cells) Uses multiple displacement amplification (MDA) Is compatible with R10.4.1 flow cells For Research Use Only

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only

This document describes the sequencing of adeno-associated viral (AAV) vectors. For Research Use Only

Human variation sequencing from 30kb extracted cell line samples using SQK-LSK114

This protocol: Uses N50 of 10 kb genomic DNA extracted from human cell lines Has sample preparation time: ~220 minutes and library preparation time: ~90 minutes Has data analysis time: 1-2 hours Requires no PCR Is compatible with R10.4.1 flow cells For Research Use Only

This protocol describes how to carry out PCR barcoding of gDNA using the Ligation Sequencing Kit V14 (SQK-LSK114) with the PCR Barcoding Expansion Pack 1-12 (EXP-PBC001) or 1-96 (EXP-PBC096). It also outlines recommendations to PCR barcode amplicons.

This document describes the barcoding of native genomic DNA libraries. For Research Use Only

This protocol describes how to carry out native barcoding of genomic DNA (gDNA) using the Native Barcoding Kit 24 V14 (SQK-NBD114.24).

For Research Use Only Document version: MLK_9193_v114_revC_15Oct2025

For Research Use Only. Document version: RRMS_9180_v114_revM_19Sep2025

For Research Use Only Document version: LWP_9183_v114_revO_25Sep2025

This protocol describes how to carry out sequencing of a Lambda control DNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114) and the Control Expansion (EXP-CTL001).

Ligation sequencing DNA V14 - dual barcoding (SQK-NBD114.24 with EXP-PBC096)

This protocol: Uses amplicon DNA Has a library preparation time of ~75 minutes Can be used with a fragmentation step (optional) Has no PCR For Research Use Only Document version: ACDE_9163_v114_revAB_24Sep2025

Ligation sequencing amplicons - Native Barcoding Kit 96 V14 (SQK-NBD114.96)

Ligation sequencing amplicons - Native Barcoding Kit 24 V14 (SQK-NBD114.24)

Library recovery from flow cells

This protocol describes an end-to-end process to prepare and sequence gDNA from cultured cell samples and analyse them using the “Human variant workflow” in EPI2ME.

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only Document version: HVSB_9230_v114_revA_24Oct2025

This protocol describes an end-to-end process to prepare and sequence gDNA from human saliva samples, and analyse the data using the wf-human-variation workflow in EPI2ME.

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only Document version: HVSW_9217_v114_revB_11Sep2025

This is an end-to-end method outlining sample extraction, library preparation, sequencing, and data analysis.

Wash and reuse MinION™ and PromethION™ Flow Cells. The EXP‑WSH004 kit provides reagents for effective library removal and sequential runs. Compatible with all DNA kits.

Direct RNA sequencing (SQK-RNA004-XL)

Direct RNA sequencing (SQK-RNA004)

Direct RNA sequencing - sequence-specific (SQK-RNA004)

Direct RNA sequencing - control experiment (SQK-RNA004)

A rapid method to perform sequencing and analysis of Salmonella.

This protocol describes an end-to-end process to prepare and sequence gDNA for chromatin accessibility from cell samples, and to analyse the data using the information outlined in our chromatin accessibility data and tool release on the EPI2ME page.

This document introduces the user to library preparation for sequencing on Oxford Nanopore devices.

This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit XL (SQK-LSK114-XL). We have developed this automated protocol on the Tecan® DreamPrep® NGS liquid handling robot.

This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit XL (SQK-LSK114-XL). We have developed this automated protocol on the Hamilton NGS STAR 96 liquid handling robot.