Resources: Documentation

This protocol uses genomic DNA - Library preparation time ~65 minutes - Fragmentation optional - No PCR - Compatible with R10.4.1 flow cells For Research Use Only

This protocol uses genomic DNA - Library preparation time ~120 minutes - Fragmentation optional - No PCR required - Suitable for processing multiple samples simultaneously, or automated library preparation - Compatible with R10.4.1 flow cells For Research Use Only

Extraction of ultra-high molecular weight (uHMW) gDNA - Reliably generate and sequence ultra-long read length N50s (>50 kb) - High yield - Compatible with R10.4.1 flow cells For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

For Research Use Only This is a registration-based Early Access product.

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10X Genomics GEM-X Single Cell 3' Kit (V4), Next-GEM Single Cell 3' Kit (V3.1), or the Visium Spatial Gene Expression Kit (V1) - Library preparation time ~205 minutes - High output - PCR required For Research Use Only

For Research Use Only

For Research Use Only.

The fastest and simplest protocol to sequence plasmid DNA - For multiplexing up to 96 samples - Library preparation time ~60 minutes - High yield - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only

The fastest and simplest protocol to sequence amplicon DNA - For multiplexing up to 96 single species amplicon samples - Optimised for 500 bp – 5 kb amplicons. - Library preparation time ~60 minutes - High yield - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only

The fastest and simplest protocol for genomic DNA involving: - ~10 mins library prep - Fragmentation - No third-party ligase needed - No PCR __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__ For Research Use Only

This protocol: - is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. - consists of a two-in-one method that splits sample into bacterial/fungal sample preparation and viral/acellular sample preparation. - provides a recommended host depletion and extraction method for respiratory samples. - uses a shotgun approach to RT-PCR. - involves tagmentation, barcoding and PCR amplification. - is compatible with R10.4.1 flow cells. <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

This protocol uses genomic DNA - For multiplexing 1-96 samples - Library preparation time ~60 minutes - High yield - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only

This protocol: - uses genomic DNA - has a low input requirement - method involves tagmentation, barcoding and PCR amplification - allows multiplexing of 1–24 samples - is compatible with R10.4.1 flow cells <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

A protocol for amplifying the 16S rRNA gene from extracted gDNA. - Genus-level bacterial identification - Multiplex up to 24 different samples - Compatible with R10.4.1 flow cells only For Research Use Only

The fastest and simplest protocol for genomic DNA involving: - ~10 mins library prep - fragmentation - no third-party ligase needed __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__ For Research Use Only

This protocol uses extracted RNA samples - Includes reverse transcription and tiled PCR amplification - For multiplexing 1-96 samples - Library preparation time ~315 minutes - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

The fastest and simplest protocol for full-length cDNA sequencing - Offering highest yield - Higher yields than traditional cDNA synthesis - Splice variants and fusion transcripts - Compatible with R10.4.1 flow cells <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

The fastest and simplest protocol for full-length cDNA sequencing - Offering highest yield - Higher yields than traditional cDNA synthesis - Splice variants and fusion transcripts - Multiplex up to 24 different samples - Compatible with R10.4.1 flow cells only <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

End-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: * Uses genomic DNA * Enables multiplexing of 4-24 samples * Takes ~60 minutes for library preparation * Includes DNA fragmentation * Is optimised for high output * Is compatible with R10.4.1 flow cells <br> __For Research Use Only__

This protocol uses genomic DNA - Hands-on setup time ~30 minutes - Automated run time ~3 hours 40 minutes - Throughput: Process 6–96 samples for multiplex library loading - Compatible with R10.4.1 Flow Cells For Research Use Only

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10X Genomics GEM-X Single Cell 5' Kit (V3) or the Next GEM Single Cell 5' Kit (V2) - Library preparation time ~135 minutes - High output - PCR required For Research Use Only

Method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: * uses human cfDNA * is for singleplex sequencing * is compatible with R10.4.1 flow cells <br> __For Research Use Only__

Method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: * uses human cfDNA * enables multiplexing of 12 samples * is compatible with R10.4.1 flow cells <br> __For Research Use Only__

The PCR-free protocol for full-length cDNA offers: - Higher yields than traditional cDNA synthesis - Analysis of splice variants & fusion transcripts - Compatibility with R10.4.1 flow cells For Research Use Only

This protocol uses extracted RNA samples - Includes reverse transcription and PCR amplification using two separate primer schemes for Influenza A and Influenza B - Includes quantification and normalisation steps to ensure equal distribution of barcodes for 24, 48 and 96 samples. - Compatible with R10.4.1 flow cells <br> For Research Use Only

- This protocol uses genomic DNA - Has very low input requirements (e.g. single cells) - Uses multiple displacement amplification (MDA) - Is compatible with R10.4.1 flow cells For Research Use Only

End-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from human blood samples - Takes ~300 minutes for sample preparation and ~90 minutes for library preparation. - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

Sequencing of adeno-associated virus (AAV) vectors. * Requires the Native Barcoding Kit 24 V14 (SQK-NBD114.24) * Includes no PCR steps * Uses up to 24 barcodes * Allows analysis of native DNA * Compatible with R10.4.1 flow cells For Research Use Only

This protocol uses N50 of 30 kb genomic DNA extracted from human cell lines * Sample preparation time: ~320 minutes and library preparation time: ~90 minutes * Data analysis: 1-2 hours * No PCR * Compatible with R10.4.1 flow cells For Research Use Only

This protocol uses N50 of 10 kb genomic DNA extracted from human cell lines - Sample preparation time: ~220 minutes and library preparation time: ~90 minutes - Data analysis: 1-2 hours - No PCR - Compatible with R10.4.1 flow cells For Research Use Only

This protocol: - Requires the PCR Barcoding Expansion (EXP-PBC001 or EXP-PBC096) with the Ligation Sequencing Kit V14 (SQK-LSK114) - Barcodes gDNA or amplicons - Has a library preparation time ~155 minutes + PCR - Results in high yield - Uses up to 96 barcodes - Includes PCR steps - Is compatible with R10.4.1 flow cells For Research Use Only

Barcoding of native genomic DNA libraries - Requires the Native Barcoding Kit 96 V14 (SQK-NBD114.96) - PCR-free protocol - Using up to 96 barcodes - Allows analysis of native DNA - Compatible with R10.4.1 flow cells For Research Use Only

Barcoding of native genomic DNA libraries - Requires the Native Barcoding Kit 24 V14 (SQK-NBD114.24) - PCR-free protocol - Using up to 24 barcodes - Allows analysis of native DNA - Compatible with R10.4.1 flow cells For Research Use Only

For barcoding of native genomic DNA libraries - Requires the Multiplex Ligation Sequencing Kit V14 XL - No PCR required - Features 96 unique barcodes - Enables low-plex sequencing - Allows analysis of native DNA - Compatible with R10.4.1 flow cells <br> For Research Use Only

For Research Use Only.

This protocol: - requires the PCR Expansion (EXP-PCA001) with the Ligation Sequencing Kit V14 (SQK-LSK114) - uses genomic DNA, amplicons or cDNA - results in high yield - has a library preparation time ~140 minutes + PCR - includes PCR steps - is compatible with R10.4.1 flow cells For Research Use Only

This protocol uses Lambda control DNA - Library preparation time ~65 minutes - Fragmentation optional - No PCR - Compatible with R10.4.1 flow cells For Research Use Only

For barcoding genomic or amplicon DNA for nanopore sequencing. - Offering highest accuracy - Multiplexing up to 2,304 samples - Requires PCR steps - Compatible with R10.4.1 flow cells <br> For Research Use Only

This protocol uses amplicon DNA - Library preparation time ~75 minutes - Fragmentation optional - No PCR For Research Use Only

Barcoding of amplicon libraries - Requires the Native Barcoding Kit 96 V14 (SQK-NBD114.96) - Includes no PCR steps - Using up to 96 barcodes - Allows analysis of native DNA - Compatible with R10.4.1 flow cells For Research Use Only

Barcoding of amplicon libraries - Requires the Native Barcoding Kit 24 V14 (SQK-NBD114.24) - Includes no PCR steps - Using up to 24 barcodes - Allows analysis of native DNA - Compatible with R10.4.1 flow cells For Research Use Only

This protocol is for recovering DNA libraries from flow cells for continued sequencing - Compatible with both MinION and PromethION flow cells, and all DNA sequencing kits For Research Use Only

End-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from human saliva samples - Takes ~300 minutes for sample preparation and ~90 minutes for library preparation. - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

End-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from human buccal swab samples - Takes ~90 minutes for sample preparation and ~135 minutes for library preparation. - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

For Research Use Only

This protocol: - Is for sequencing native RNA - Can be used with total RNA or an enriched sample (e.g. poly(A) tailed or ribo-depleted) as a starting input material - Requires no fragmentation - Takes approximately 140 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__

This protocol: - Is for sequencing native RNA - Can be used with total RNA or an enriched sample (e.g. poly(A) tailed or ribo-depleted) as a starting input material - Requires no fragmentation - Takes approximately 140 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__

This protocol: - Is for selecting RNA targets of your choice for native RNA sequencing - Uses total RNA as a starting input material - Requires no fragmentation - Takes approximately 140 minutes for library preparation __For Research Use Only__

This protocol: - Is for a control experiment using kit-supplied RNA - Sequences native RNA - Requires no fragmentation - Takes approximately 140 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__

This protocol: - is a rapid method to perform sequencing and analysis of *Salmonella*. - uses *Salmonella* colonies from overnight cultures. - includes tagmentation, barcoding and PCR amplification. - enables multiplexing of 1-24 samples. - is compatible with R10.4.1 flow cells. <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

Chemistry Technical Document

This protocol uses genomic DNA - Hands-on setup time ~30 minutes - Automated run time ~2 hours 40 minutes - Throughput: Process 8–96 samples for singleplex library loading - Compatible with R10.4.1 Flow Cells For Research Use Only

This protocol uses genomic DNA - Automation of library preparation - Increased reproducibility and speed - Reduces human error - High sequencing output - Library preparation time ~1.5 hours hands-on-time and ~3-4 hours automation time - Fragmentation is optional - No PCR required - Multiple samples can be prepared simultaneously - Compatible with R10.4.1 flow cells For Research Use Only