Resources: Documentation

Extraction of ultra-high molecular weight (uHMW) gDNA - Reliably generate and sequence ultra-long read length N50s (>50 kb) - High yield - Compatible with R10.4.1 flow cells For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

For Research Use Only This is a registration-based Early Access product.

This protocol: - describes how to carry out barcoded Telo-Seq for native sequencing of telomeres. - uses high molecular weight genomic DNA (HMW gDNA). - requires custom-ordered oligos for telomere adapters and splints. - enables accurate telomere length measurement and assigns each telomere to a chromosome arm. - is compatible with R10.4.1 flow cells. <br> For Research Use Only

10X Genomics Visium Spatial transcriptomics method: - Requires cDNA amplicons produced using the Visium Spatial Gene Expression assay (Visium V1 for Fresh Frozen) or the Visium HD 3' Spatial Gene Expression assay - Library preparation time ~205 minutes - High output - PCR required For Research Use Only

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10x Genomics Chromium GEM-X Universal 3’ Gene Expression (V4) assay or the Chromium Next GEM Universal 3’ Gene Expression (V3.1) assay. - Library preparation time ~205 minutes - High output - PCR required For Research Use Only

For Research Use Only.

The fastest and simplest protocol to sequence plasmid DNA - For multiplexing up to 96 samples - Library preparation time ~60 minutes - High yield - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only

The fastest and simplest protocol to sequence amplicon DNA - For multiplexing up to 96 single species amplicon samples - Optimised for 500 bp – 5 kb amplicons. - Library preparation time ~60 minutes - High yield - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only

This protocol: - is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. - consists of a two-in-one method that splits sample into bacterial/fungal sample preparation and viral/acellular sample preparation. - provides a recommended host depletion and extraction method for respiratory samples. - uses a shotgun approach to RT-PCR. - involves tagmentation, barcoding and PCR amplification. - is compatible with R10.4.1 flow cells. <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

Automated rapid barcoding method using the ElysION device outlining library preparation and sequencing. This protocol: * Is an automated method using the ElysION device * Uses genomic DNA * Enables multiplexing of up to 96 samples * Includes DNA fragmentation * Is optimised for high output * Is compatible with R10.4.1 flow cells <br> __For Research Use Only__ This method is user with an Early Access device. For more information about our Early Access programmes, please refer to this article on [product release phases](https://nanoporetech.com/news/news-blog-you-cant-put-label-innovation-or-can-you).

This protocol provides an end-to-end workflow for the analysis of pharmacogenomic (PGx) genes using adaptive sampling, and the Oxford Nanopore platform. Downstream variant calling is via the EPI2ME™ wf-pgx workflow, acquiring a minimum of 40x coverage per sample, across the PGx-associated genes. The protocol covers genomic DNA extraction, mechanical shearing, native barcoding, library preparation, sequencing, and data analysis. It supports the multiplexing of up to four samples per PromethION™ Flow Cell and is compatible with DNA extracted from human whole blood, cultured cells, or saliva. This protocol: - Uses genomic DNA extracted from human whole blood, cultured cells, or saliva - Requires DNA fragmentation - Uses the Native Barcoding Kit 24 V14 (SQK-NBD114.24) - Is compatible with R10.4.1 flow cells - Allows the analysis of data using the wf-pgx workflow in EPI2ME <br> **For Research use only**

This protocol uses extracted RNA samples - Includes reverse transcription and tiled PCR amplification - For multiplexing 1-96 samples - Library preparation time ~315 minutes - Fragmentation - Compatible with R10.4.1 flow cells For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

End-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: * Uses genomic DNA * Enables multiplexing of 4-24 samples * Takes ~60 minutes for library preparation * Includes DNA fragmentation * Is optimised for high output * Is compatible with R10.4.1 flow cells <br> __For Research Use Only__

Automated native barcoding method using the ElysION device outlining library preparation and sequencing. This protocol: * Is an automated method using the ElysION device * Enables multiplexing of up to 48 samples * Allows analysis of native DNA * Is a PCR-free method * Is compatible with R10.4.1 flow cells <br> __For Research Use Only__ This method is used with an Early Access device. For more information about our Early Access programmes, please refer to this article on [product release phases](https://nanoporetech.com/news/news-blog-you-cant-put-label-innovation-or-can-you).

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10x Genomics Chromium GEM-X Universal 5' Gene Expression (V3) assay or the Chromium Next GEM Universal 5' Gene Expression (V2) assay - Library preparation time ~135 minutes - High output - PCR required For Research Use Only

Method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: * uses human cfDNA * is for singleplex sequencing * is compatible with R10.4.1 flow cells <br> __For Research Use Only__

Method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: * uses human cfDNA * enables multiplexing of 12 samples * is compatible with R10.4.1 flow cells <br> __For Research Use Only__

This protocol uses extracted RNA samples - Includes reverse transcription and PCR amplification using two separate primer schemes for Influenza A and Influenza B - Includes quantification and normalisation steps to ensure equal distribution of barcodes for 24, 48 and 96 samples. - Compatible with R10.4.1 flow cells <br> For Research Use Only

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from human blood samples - Takes ~300 minutes for sample preparation and ~90 minutes for library preparation - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

Sequencing of adeno-associated virus (AAV) vectors. * Requires the Native Barcoding Kit 24 V14 (SQK-NBD114.24) * Includes no PCR steps * Uses up to 24 barcodes * Allows analysis of native DNA * Compatible with R10.4.1 flow cells For Research Use Only

This protocol: - Uses N50 of 30 kb genomic DNA extracted from human cell lines - Has sample preparation time: ~320 minutes and library preparation time: ~90 minutes - Has data analysis: 1-2 hours - Requires no PCR - Is compatible with R10.4.1 flow cells For Research Use Only

This protocol: - Uses N50 of 10 kb genomic DNA extracted from human cell lines - Has sample preparation time: ~220 minutes and library preparation time: ~90 minutes - Has data analysis time: 1-2 hours - Requires no PCR - Is compatible with R10.4.1 flow cells For Research Use Only

For Research Use Only.

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from human saliva samples - Takes ~300 minutes for sample preparation and ~90 minutes for library preparation. - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from human buccal swab samples - Takes ~90 minutes for sample preparation and ~135 minutes for library preparation. - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

End-to-end method outlining sample extraction, library preparation, sequencing and data analysis. This protocol: - Uses genomic DNA extracted from whole blood, DNA - Requires no PCR - Compatible with R10.4.1 flow cells __For Research Use Only__

This protocol: - is a rapid method to perform sequencing and analysis of *Salmonella*. - uses *Salmonella* colonies from overnight cultures. - includes tagmentation, barcoding and PCR amplification. - enables multiplexing of 1-24 samples. - is compatible with R10.4.1 flow cells. <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

End-to-end method for preparing Chromatin Accessibility libraries. This protocol: - Outlines 6mA tagging - Uses genomic DNA extracted from cell samples - Takes ~340 minutes for sample preparation and ~90 minutes for library preparation. - Requires no PCR - Is compatible with R10.4.1 flow cells __For Research Use Only__

Automated end-to-end method using the ElysION device outlining sample extraction, library preparation, sequencing and analysis. This protocol: * Is an automated method using the ElysION device * Uses bacterial or fungi cells * Enables multiplexing of up to 24 samples * Includes DNA fragmentation * Is optimised for high output * Is compatible with R10.4.1 flow cells <br> __For Research Use Only__ This method is used with an Early Access device. For more information about our Early Access programmes, please refer to this article on [product release phases](https://nanoporetech.com/news/news-blog-you-cant-put-label-innovation-or-can-you).