Resources: Documentation

Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)

Telomere-to-telomere sequencing (T2T) from blood and cells using SQK-APK114, SQK-LSK114, and SQK-ULK114

This protocol describes how to carry out native telomere sequencing using the platform developed by Oxford Nanopore Technologies. Telo-Seq delivers single-telomere resolution at high throughput, enabling the multiplexing from 4 to up to 12 samples in a single run.

This is a 10X Genomics Visium Spatial transcriptomics method.

This is a single-cell transcriptomics method.

This protocol is for the extraction and sequencing of ultra-high molecular weight (uHMW) genomic DNA.

For Research Use Only.

This protocol describes how to carry out rapid barcoding of plasmid DNA using the Rapid Barcoding Kit 24 or 96 V14 (SQK-RBK114.24 or SQK-RBK114.96) to sequence up to 96 plasmid samples.

This is the fastest and simplest protocol to sequence amplicon DNA. For Research Use Only Document version: RAA_9198_v114_revM_17Oct2025

This is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. For Research Use Only

Automated rapid barcoding method using the ElysION device, outlining library preparation and sequencing.

This protocol provides an end-to-end workflow for the analysis of pharmacogenomic (PGx) genes using adaptive sampling, and the Oxford Nanopore platform.

This is a legacy method. Please refer to our new equivalent method: SARS-CoV-2 virus PCR tiling and sequencing from RNA using SQK-RBK114.96 For Research Use Only This is an Early Access product. Document version: MRT_9186_v114_revL_8Dec2025

This is an end-to-end method outlining sample extraction, library preparation, sequencing, and data analysis. This protocol: * Uses genomic DNA * Enables multiplexing of 4-24 samples on MinION and GridION or 4-96 samples on PromethION * Takes ~60 minutes for library preparation * Includes DNA fragmentation * Is optimised for high output * Is compatible with R10.4.1 flow cells <br> __For Research Use Only__

Native barcoding DNA V14 – automated ElysION (SQK-NBD114.96)

Oxford Nanopore Technologies | Welcome to Oxford Nanopore technologies. Our goal is to enable the analysis of any living thing, by any person, in any environment

This protocol describes how to carry out preparation and sequencing of a human cfDNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). Typically, we obtain ~50 Gb of aligned data (15x coverage) for human cfDNA samples processed with this protocol.

This protocol describes how to carry out preparation and sequencing of 12 human cell-free DNA (cfDNA) samples using the Native Barcoding Kit 24 V14 (SQK-NBD114.24). Typically, we obtain ~3 Gb of aligned data (1x coverage) for each of the 12 human cfDNA samples processed with this protocol.

This document describes the sequencing of the influenza virus. For Research Use Only

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only

This document describes the sequencing of adeno-associated viral (AAV) vectors. For Research Use Only

Human variation sequencing from 30kb extracted cell line samples using SQK-LSK114

This protocol: Uses N50 of 10 kb genomic DNA extracted from human cell lines Has sample preparation time: ~220 minutes and library preparation time: ~90 minutes Has data analysis time: 1-2 hours Requires no PCR Is compatible with R10.4.1 flow cells For Research Use Only

For Research Use Only. Document version: RRMS_9180_v114_revM_19Sep2025

This protocol describes an end-to-end process to prepare and sequence gDNA from cultured cell samples and analyse them using the “Human variant workflow” in EPI2ME.

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only Document version: HVSB_9230_v114_revA_24Oct2025

This protocol describes an end-to-end process to prepare and sequence gDNA from human saliva samples, and analyse the data using the wf-human-variation workflow in EPI2ME.

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only Document version: HVSW_9217_v114_revB_11Sep2025

This is an end-to-end method outlining sample extraction, library preparation, sequencing, and data analysis.

This is a rapid method to perform sequencing and analysis of *Salmonella*. The protocol: - Uses *Salmonella* colonies from overnight cultures - Includes tagmentation, barcoding, and PCR amplification - Enables multiplexing of 1-24 samples - Is compatible with R10.4.1 flow cells **For Research Use Only**

This protocol describes an end-to-end process to prepare and sequence gDNA for chromatin accessibility from cell samples, and to analyse the data using the information outlined in our chromatin accessibility data and tool release on the EPI2ME page.

This end to end protocol describes our automated Nanopore-Only Microbial Isolate Sequencing Solution (NO-MISS): a flexible approach allowing sequencing of up to 24 microbial isolate genomes per MinION Flow Cell, generating a coverage of 50x per genome.

This protocol aims to rapidly produce libraries with a read N50 of ~30 kb and generate ≥30x coverage of the genome, thereby providing sufficient data to robustly call small and large variants, as well as information on methylation and phasing.