Resources: Documentation
Sample storage
Cell storage
Last updated 9/15/2023
Blood storage
Last updated 9/15/2023
Extraction protocols
Automated high molecular weight gDNA extraction from blood
Last updated 9/15/2023
High output, long read sequencing from human blood
Last updated 2/29/2024
DNA/RNA Handling
RNA stability
Last updated 9/15/2023
The effect of varying the number of PCR cycles in the PCR-cDNA Sequencing Kit
Last updated 9/15/2023
Library prep protocols
Ligation sequencing amplicons - native barcoding (SQK-LSK109 with EXP-NBD104 and EXP-NBD114)
Last updated 3/10/2023
Ligation sequencing gDNA - Cas9 enrichment (SQK-CS9109)
Last updated 11/1/2023
SARS-CoV-2
PCR tiling of SARS-CoV-2 virus - Rapid Barcoding Kit 96 V14 and Midnight RT PCR Expansion (SQK-RBK114.96 and EXP-MRT001)
Last updated 9/17/2024
PCR tiling of SARS-CoV-2 virus - automated Agilent (SQK-RBK110.96 with EXP-MRT001)
Last updated 6/29/2023
Automation
Ligation sequencing gDNA - automated Hamilton NGS STAR 96 (SQK-LSK109-XL)
Last updated 7/12/2023
Loading multiple PromethION Flow Cells
Last updated 2/7/2024
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