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Whole-genome sequencing with nanopore technology

Generate and complete microbial, human, animal, and plant genome assemblies with long nanopore sequencing reads (reads in excess of 4 Mb have been demonstrated). The greater overlap offered by long sequencing reads enhances genome assembly by providing longer continuous, unambiguously assembled sequences, resulting in fewer contigs. Accurately resolve structural variants and repeat regions, and characterise base modifications (e.g. methylation), fusion genes, and haplotype phasing, with nanopore long reads.

  • Resolve structural variants, repeat regions, and phasing with long reads (up to 4.2 Mb demonstrated)
  • Simplify de novo assembly and correct existing reference genomes
  • Rapid, streamlined library prep workflows with low input requirements
  • Explore epigenetic modifications and eliminate bias with direct sequencing of native DNA
  • Stop sequencing when sufficient depth is obtained with real-time basecalling and analysis
  • Scale to your needs using Flongle, MinION, GridION, or PromethION

How will you use nanopore technology?

Microbial genomes

Human genomes

Animal genomes

Plant genomes

Obtain complete bacterial, viral, and fungal DNA or RNA genome assemblies using long nanopore sequencing reads. Perform accurate species identification and genome assembly of single strains or complex metagenomic samples, at the bench or in the field. Comprehensively analyse challenging genomic regions, such as structural variants and repeat sequences, to obtain high-quality assemblies.

  • Accurately resolve structural variants and repeats using long sequencing reads
  • Obtain complete genomes and correct reference assemblies with de novo or reference-guided approaches
  • Directly sequence RNA viral genomes, avoiding bias
  • Rapidly perform comprehensive pathogen detection and strain characterisation with real-time analysis – and at the point of outbreak with the portable MinION and Flongle
  • Directly identify epigenetic modifications (e.g. methylation) alongside nucleotide sequence 
  • Sequence entire plasmids and viruses in single reads, eliminating the need for assembly

Obtain complete telomere-to-telomere assemblies of the human genome using long and ultra-long nanopore reads (up to 4.2 Mb demonstrated). Fully characterise human genetic variation by accurately resolving structural variants and breakpoints, phasing haplotypes, and sequencing across challenging repeat regions, such as centromeres. Perform individual or population-scale whole-genome sequencing studies with a range of nanopore sequencing platforms to suit your needs.

* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.

Complete genome assemblies of model and non-model organisms using long nanopore sequencing reads. Gain unprecedented understanding of genetic variation and evolution across animal species to advance disease research and positively impact animal breeding. Fully characterise both animal host and pathogen genomes, including resolution of structural variants, repeat regions, and transposable elements, using long and ultra-long nanopore reads.

* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.

Plant genomes are challenging to sequence and assemble using short-read sequencing technologies as they are characteristically large, highly repetitive, and exhibit a variety of ploidy. Long and ultra-long nanopore reads enhance plant genome assembly and enable the resolution of large structural variants, repeat regions, and transposable elements.—

* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.

'The PromethION is a real game changer, combining ultra-long reads with high sequence output for the production of contiguous, high-quality reference genomes. Using this platform, we sequenced the 2.56 Gb lettuce genome at >100X coverage using just a few flow cells.' 

Alexander Wittenberg, KeyGene, PromethION service provider

Elizaveta Grigoreva: Whole-genome assembly of guar (Cyamopsis tetragonoloba [L.] Taub.) by second- and third-generation sequencing approaches