Whole-genome sequencing with nanopore technology
Generate and complete microbial, human, animal, and plant genome assemblies with long nanopore sequencing reads (reads in excess of 4 Mb have been demonstrated). The greater overlap offered by long sequencing reads enhances genome assembly by providing longer continuous, unambiguously assembled sequences, resulting in fewer contigs. Accurately resolve structural variants and repeat regions, and characterise base modifications (e.g. methylation), fusion genes, and haplotype phasing, with nanopore long reads.
- Resolve structural variants, repeat regions, and phasing with long reads (up to 4.2 Mb demonstrated)
- Simplify de novo assembly and correct existing reference genomes
- Rapid, streamlined library prep workflows with low input requirements
- Explore epigenetic modifications and eliminate bias with direct sequencing of native DNA
- Stop sequencing when sufficient depth is obtained with real-time basecalling and analysis
- Scale to your needs using Flongle, MinION, GridION, or PromethION
How will you use nanopore technology?
Microbial genomes
Human genomes
Animal genomes
Plant genomes
Obtain complete bacterial, viral, and fungal DNA or RNA genome assemblies using long nanopore sequencing reads. Perform accurate species identification and genome assembly of single strains or complex metagenomic samples, at the bench or in the field. Comprehensively analyse challenging genomic regions, such as structural variants and repeat sequences, to obtain high-quality assemblies.
- Accurately resolve structural variants and repeats using long sequencing reads
- Obtain complete genomes and correct reference assemblies with de novo or reference-guided approaches
- Directly sequence RNA viral genomes, avoiding bias
- Rapidly perform comprehensive pathogen detection and strain characterisation with real-time analysis – and at the point of outbreak with the portable MinION and Flongle
- Directly identify epigenetic modifications (e.g. methylation) alongside nucleotide sequence
- Sequence entire plasmids and viruses in single reads, eliminating the need for assembly
'Nanopore sequencing provides low-cost long-read data suited to closure of complex genomes including plasmids. Moreover, the preparation of the libraries is quick and easy, and the samples can be sequenced in a short time directly in the same laboratory, without the need for external facilities'
Baseggio, L. et al. Microb Genom. 7:4 (2021).
Nicole Wagner: Evolutionary history of conical stromatolites
Obtain complete telomere-to-telomere assemblies of the human genome using long and ultra-long nanopore reads (up to 4.2 Mb demonstrated). Fully characterise human genetic variation by accurately resolving structural variants and breakpoints, phasing haplotypes, and sequencing across challenging repeat regions, such as centromeres. Perform individual or population-scale whole-genome sequencing studies with a range of nanopore sequencing platforms to suit your needs.
- Obtain complete and contiguous genome assemblies using de novo or reference-guided approaches
- Identify and comprehensively characterise disease-specific variants in clinical research and cancer research samples with rapid access to results
- Accurately resolve structural variants, breakpoints, and repeat regions
- Investigate haplotype phasing and gene linkage
- Detect epigenetic modifications with direct sequencing and eliminate PCR bias
- Scale to your requirements — up to 2.8 Gb Flongle; 50 Gb MinION; 250 Gb GridION; 580 Gb PromethION 2; 14 Tb PromethION 48*
* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.
'We’ve demonstrated the impact of ultra-long reads on assembly contiguity and their facility to resolve areas of the genome that have proven intractable to short-read sequencing, including telomeres, centromeres and highly variable regions'
Euan Ashley: The potential of ultrarapid nanopore genome sequencing for critical care medicine
Complete genome assemblies of model and non-model organisms using long nanopore sequencing reads. Gain unprecedented understanding of genetic variation and evolution across animal species to advance disease research and positively impact animal breeding. Fully characterise both animal host and pathogen genomes, including resolution of structural variants, repeat regions, and transposable elements, using long and ultra-long nanopore reads.
- Obtain complete genomes and correct reference assemblies with de novo or reference-guided approaches
- Accurately resolve structural variants, repeat regions, and phasing across the entire genome
- Rapidly sequence and characterise animal pathogens, at the bench, or in the field with the portable MinION and Flongle
- Detect epigenetic modifications using direct sequencing — and eliminate PCR bias
- Scale to your requirements — up to 2.8 Gb Flongle; 50 Gb MinION; 250 Gb GridION; 14 Tb PromethION*
* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.
'Long reads are required to generate high-quality genome assemblies from which you can truly understand gene synteny, allowing much more accurate lineage analysis of related species — this just can’t be done using short reads'
Lara Urban: Leveraging adaptive sampling of environmental DNA for monitoring the critically endangered kākāpō
Plant genomes are challenging to sequence and assemble using short-read sequencing technologies as they are characteristically large, highly repetitive, and exhibit a variety of ploidy. Long and ultra-long nanopore reads enhance plant genome assembly and enable the resolution of large structural variants, repeat regions, and transposable elements.—
- Accurately resolve large structural variants and repeats
- Assemble complete genomes and correct references with de novo or reference-guided approaches
- Rapidly detect and characterise plant pathogens in real time from simple or complex metagenomic samples — and in the field with the portable MinION and Flongle
- Directly identify epigenetic modifications and eliminate PCR bias
- Investigate haplotype phasing and gene linkage
- Scale to your requirements — up to 2.8 Gb Flongle; 50 Gb MinION; 250 Gb GridION; 14 Tb PromethION*
* Theoretical max output when system is run for 72 hours (or 16 hours for Flongle) at 420 bases / second. Outputs may vary according to library type, run conditions, etc.
'The PromethION is a real game changer, combining ultra-long reads with high sequence output for the production of contiguous, high-quality reference genomes. Using this platform, we sequenced the 2.56 Gb lettuce genome at >100X coverage using just a few flow cells.'
Alexander Wittenberg, KeyGene, PromethION service provider
Elizaveta Grigoreva: Whole-genome assembly of guar (Cyamopsis tetragonoloba [L.] Taub.) by second- and third-generation sequencing approaches