A guide to microbial sequencing with Oxford Nanopore
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Overview
Full characterisation of microbial genomes is advancing numerous fields of research such as the impact of the human microbiome on health and disease. Sequencing pathogens behind infectious disease outbreaks is essential to improving our understanding of their origins, transmission, antimicrobial resistance (AMR) profiles, and virulence factors.
In this getting started guide, find out how nanopore sequencing can characterise complete, contiguous, high-quality microbial genomes for timely detection of pathogens, including plant and animal microbes that impact animal welfare and food production.
In this getting started guide, you will:
- Find out how nanopore reads of any length enhance de novo genome assembly and microbial characterisation
- Discover the benefits of nanopore sequencing
- Identify the appropriate nanopore sequencing kit for your experiment
- For any questions, see the FAQs for this technique
- Read real-world case studies of how researchers are utilising nanopore sequencing to accurately analyse known and novel microbes, as well as precisely differentiate closely related microorganisms, and reconstruct complete plasmids