Chromatin conformation
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A complete, end-to-end workflow for chromosome conformation capture
Chromatin conformation capture (3C) techniques reveal genomic interactions in three dimensions. This can provide key information on the effect of chromatin structure on transcriptional regulation; the data can also be utilised to orient contigs, producing highly contiguous scaffolded assemblies.
The traditionally used short-read 3C methodology limits the number of contacts available to analyse per read. Combining chromatin conformation capture with long Oxford Nanopore reads, Pore-C provides long-range contact information, shedding light on higher-order structure. The technique is PCR free, allowing the characterisation of base modifications in the same dataset.
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Unprecedented access to haplotype-resolved biology
Discover how the combination of long, amplification-free nanopore sequencing reads and highly accurate SNP calling enables co-phasing of variants and imprinted regions for parent-of-origin inference from a single sample.
Haplotype-specific regulation of chromatin fibres
Nick Owens discusses how he utilised the multiomic capabilities of Oxford Nanopore sequencing to resolve genotype with phased DNA methylation and open chromatin simultaneously.
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Recommended device for chromatin conformation
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PromethION 2 devices
Offering the flexibility of two independent, high-output PromethION Flow Cells, the compact PromethION 2 devices bring the benefits of high-coverage, real-time nanopore sequencing to every lab. Ideal for low-cost access to long and ultra-long reads suitable for chromatin conformation studies and highly accurate whole-genome assemblies.
