Nanopore DNA/RNA analysis is being used to understand the role of genomic information on human health.
The MinION - the portable, long read, real time DNA sequencer – has been used to sequence complete human genomes for the first time, and is in use in other areas of human genetics.
And now, the GridION is available to enable up to 5 MinION Flow Cells for 50-100Gb of DNA sequence data. The high-throughput, high sample-number, on-demand PromethION has a modular design and is designed for 280x the sequencing capacity of MinION.
What does nanopore sequencing offer population genomics?
- de novo genome assembly of model and non-model organisms
- resequencing aids demographic studies, evolution of species and local adaptation
Nanopore sequencing on the MinION gives you:
Real-time data, rapid results
Nanopore sequencing data starts streaming immediately, rather than being delivered in bulk at the end of a 'run'. Real-time data streaming allows immediate analysis of the data, enabling users to perform assembly and mapping / alignment quickly, even during the experiment.Publications on rapid identification
Preparation of sequencing libraries from genomic DNA is simple and takes about ten minutes with the latest kit. Oxford Nanopore is developing new devices to simplify this further.
Real-time data analysis means you can make your workflow more efficient. You can stop sequencing when an answer has been reached and move on to the next experiment.Publications on simple workflow
While the MinION weighs under 100g and can be used in the field or as a 'personal sequencer', the larger GridION and PromethION devices are designed for larger scale analyses. GridION enables real time sequencing in up to 5 MinION Flow Cells, with integrated compute. PromethION is designed to deliver nearly 300 times the sequencing power of MinION.Publications on transportable labs
Efficient genome assembly with long reads
The Oxford Nanopore system processes the library strands of DNA that are presented to it, regardless of their length. Users can choose long reads of kilobases to ultra-long reads of hundreds of kilobases, simplifying the data analysis workflow.Publications on long reads