Software downloads

Features

• Data Pooling: Added initial support for pooling data across multiple flow cells.
• Updated Basecalling Models: Upgraded to v5.2 DNA basecalling models, delivering more accurate HAC performance and faster all-context modified basecalling. HAC modified basecalling (CpG context) with Adaptive Sampling supported under selected conditions.
• Interruptible Protocols: Added automatic pausing of protocols when a flow cell was disconnected or when disk space was insufficient to continue sequencing.
• Run Until Enhancements: Added flexible run control based on target read counts and extended barcode-level balancing through the updated Run Until by Barcode Read Counts feature. (Not available in Standalone mode.)
• Intermediate Read Recovery: Improved handling and recovery of POD5 reads following unexpected interruptions or crashes.

Improvements

• Added hash file generation to to allow data integrity validation.
• Improved pore count accuracy to better reflect active pores after washes.
• Experiment joining has been restricted to the last 30 days, and older MinKNOW UI versions have been marked as incompatible to prevent the use of incompatible settings across versions.
• Added RNA barcoding support ahead of the upcoming kit release.
• Added sample-sheet validation to prevent mixed library types in a single sheet.
• Removed excess read groups from BAM files when splitting by barcode.

Bug Fixes

• Improved adaptive sampling stability..
• Fixed an issue where NFS drives remained mounted.
• Corrected UI temperature error messaging.
• Fixed missing reference-size warnings in applicable circumstances.
• Removed 40-character limit on network-mount paths.
• Increased demultiplexing barcode list size in the UI.
• Fixed mismatch between the number of reads shown in the Experiments header and table.
• Updated BAM File Headers and Metadata Tag Specification.
• Improved offload speed accuracy to better represent true transfer rates.
• Fixed drop-off in basecall speed when using multiple model types concurrently.
• Adjusted saturation control to minimise incorrect channel loss.

Announcements

• This is the last major Mk1C release was 25.05; no subsequent releases followed.
• Removed support for FLO-MIN106 and FLO-PRO002.
• Removed support for SQK-RNA002.
• Removed FAST5 format support in MinKNOW.
• Removed duplex basecalling support.

Raw reads are now off by default.