Software downloads

Features

• New Dorado Barcoding Method: Includes reduced unclassified rates with RBK114 kits.
• Support Link from MinKNOW
• File Output Format Option Updates: New default option for file output by time (1and 10 min, hourly and end of the run). Demultiplexing for POD5 off by default
• RNA Script and Model Updates: Short read mode and m6A (DRACH context) basecalling model.
• New Data Offload Functionality
• New v4.3 Basecalling Models: Improved accuracy particularly in telomeric, low complexity regions and for bacterial samples. Release also Includes all-context 5mC/5hmC and 6mA models.
• Q-Accuracy Graph: Available when alignment is used.
• MinKNOW in Spanish: ¡MinKNOW ya está disponible en español!

Improvements

• Basecalling: Speed up at the start of a run.
• Script Updates for DNA Sequencing: Increased output for short fragments.
• Adaptive Sampling Updates: Faster rejection time and better classification of reads to be rejected.
• Hub and Spoke Run Setup in MinKNOW
• Improved Alignment Speed
• Enhanced Security: Remote access will be switched off by default and users will need to manually enable it.
• Front-End Enhancements: New statistics and settings on the Q score histogram and Q accuracy graph in the MinKNOW UI, the basecall model used is now recorded in the run report, which also includes the Q-score histogram and the number of passed and failed reads.
• Read Splitting: Defaulted to on for all conditions; options to change this have been removed for UI simplification.
• Barcode Alias: Alias used in sample sheet is now included in both directory name and file name. Improved System Update Experience
• SQK-ULK114 Kit Update: Allows more time for reads to finish before a pore scan.
• Low Disk Space Warnings: Warning provided at run setup

Bug Fixes

• Saturation Issues on P2 Integrated: Reduction in frequency of the Issue
• UI Freezing: Fixed issue when left on the experiments page.
• Better Reboot Instructions: For when users need to reboot their device.
• Cropped Barcode Names in Report
• Basecall hanging: Fixed an Issue where the basecalling would hang at 99%
• Date and Time Management Improvements
• Run Report Target Temperature: Now correctly reflects the set temperature.