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Whole-genome sequencing with nanopore technology

Complete microbial, human, animal, and plant genomes with long-read nanopore sequencing. Greater overlap between reads enhances genome assembly by providing longer continuous sequences and fewer contigs. Accurately resolve structural variants and repeat regions, and characterise base modifications, fusion genes, and haplotype phasing, with nanopore long reads.

  • Resolve structural variants, repeat regions and phasing with long reads
  • Simplify de novo assembly and correct existing genomes
  • Streamlined, rapid library prep workflows, and low input requirements
  • Explore epigenetic modifications and eliminate bias with direct sequencing
  • Stop sequencing when sufficient depth is obtained with real-time basecalling and analysis
  • Scale to your needs using Flongle, MinION, GridION, or PromethION

How will you use nanopore technology?

Microbial genomes

Human genomes

Animal genomes

Plant genomes

Obtain complete bacterial, viral, and fungal DNA or RNA genomes, with nanopore long sequencing reads. Perform accurate species identification and genome assembly of complex metagenomic samples, at the bench or in the field. Comprehensively analyse challenging genomic regions, such as structural variants and repeat sequences, to obtain high-quality assemblies.

  • Accurately resolve structural variants and repeats using long sequencing reads
  • Obtain complete genomes and correct reference assemblies with de novo or reference guided approaches
  • Directly sequence RNA viral genomes, avoiding bias
  • Rapidly perform comprehensive pathogen detection and strain characterisation with real-time analysis – and at the point of outbreak with the portable MinION
  • Directly identify epigenetic modifications alongside nucleotide sequence 
  • Sequence entire plasmids and viruses in single reads, eliminating the need for assembly

'Genome assembly using short-reads is challenged by repetitive sequences and extreme GC contents. Our results indicate that these difficulties can be largely overcome by using single-molecule, long-read sequencing technologies such as the Oxford Nanopore MinION.' 

Goldstein et al
 

Natalie Ring: how sequencing is changing Bordetella pertussis research

Obtain complete telomere-to-telomere assemblies of the human genome using nanopore long and ultra-long reads. Fully characterise human genetic variation, by accurately resolving structural variants and breakpoints, phasing haplotypes, and sequencing across challenging repeat regions, such as centromeres. Perform individual or population-scale whole-genome sequencing studies with a range of nanopore sequencing platforms to suit your needs.

  • Obtain complete and contiguous genome assemblies using de novo or reference guided approaches
  • Identify and comprehensively characterise disease-specific variants in clinical research samples, with rapid access to results
  • Accurately resolve structural variants, breakpoints and repeat regions
  • Investigate haplotype phasing and gene linkage
  • Detect epigenetic modifications with direct sequencing and eliminate PCR bias
  • Scale to your requirements – 30 Gb MinION; 150 Gb GridION; 4,800/9,600 Gb PromethION P24/P48

'We’ve demonstrated the impact of ultra-long reads on assembly contiguity and their facility to resolve areas of the genome that have proven intractable to short-read sequencing, including telomeres, centromeres and highly variable regions'

Dr Matt Loose, University of Nottingham

Sara Goodwin: Exploring the architecture of organoid genomes with PromethION technology

Complete genome assemblies of model and non-model organisms using nanopore long reads. Gain unprecedented understanding of genetic variation and evolution across animal species to advance disease research and positively impact animal breeding. Fully characterise both host and pathogen genomes, including resolution of structural variants, repeat regions, and transposable elements, using nanopore long and ultra-long reads.

  • Obtain complete genomes and correct reference assemblies with de novo or reference guided approaches
  • Accurately resolve structural variants, repeat regions and phasing
  • Rapidly sequence and characterise animal pathogens, at the bench, or in the field with the portable MinION
  • Detect epigenetic modifications using direct sequencing – and eliminate PCR bias
  • Scale to your requirements – 30 Gb MinION; 150 Gb GridION; 4,800/9,600 Gb PromethION P24/P48

Plant genomes are challenging to sequence and assemble using short-read sequencing technologies as they are characteristically large, highly repetitive, and exhibit a variety of ploidy. Nanopore long and ultra-long reads enhance plant genome assembly and enable the resolution of large structural variants, repeat regions, and transposable elements.

  • Accurately resolve large structural variants and repeats
  • Assemble complete genomes and correct references with de novo or reference guided approaches
  • Rapidly detect and characterise plant pathogens in real time from simple or complex metagenomic samples – and in the field with the portable MinION
  • Directly identify epigenetic modifications and eliminate PCR bias
  • Investigate haplotype phasing and gene linkage
  • Scale to your requirements – 30 Gb MinION; 150 Gb GridION; 4,800/9,600 Gb PromethION P24/P48

'The PromethION is a real game changer, combining ultra-long reads with high sequence output for the production of contiguous, high-quality reference genomes. Using this platform, we sequenced the 2.56 Gb lettuce genome at >100X coverage using just a few flow cells.' 

Alexander Wittenberg, KeyGene, PromethION service provider

Todd Michael: Unraveling the mysteries of CBD and THC content with a chromosome resolved Cannabis genome

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