Environmental research and sequencing with nanopore technology
Nanopore technology delivers unique opportunities for environmental research — from portable, in-field sequencing and sample prep using MinION, Flongle, and VolTRAX devices, to high-throughput analysis of high sample numbers using GridION and PromethION. Critically, nanopore DNA and RNA sequencing delivers all data in real time, providing immediate access to actionable results. High yields combined with sample multiplexing provides highly cost-efficient analyses.
- Sequence at sample source using powerful and portable MinION sequencing devices
- Get immediate access to actionable results with real-time data streaming and analysis workflows
- Enhance genome and metagenome analysis using direct, long sequencing reads, with simultaneous detection of base modifications
- Streamline your research with 10-minute library prep (DNA)/105-minute (direct RNA)
- Prepare samples in the field or lab using the USB-powered VolTRAX device.
- Scale to your needs using Flongle, MinION, GridION, or PromethION
How will you use nanopore technology?
Environmental DNA (eDNA)
Microbiome analysis
Conservation and biodiversity
Plant and animal pathogens
Biodetection and biosurveillance
- Accurately identify microorganisms and discriminate closely related species using long nanopore sequencing reads
- Sequence samples at source with fully portable MinION devices
- Get rapid insights using real-time data streaming and analysis workflows
- Design your optimal workflow with our online protocol builder
- Streamline your workflow with 10-min manual library prep or hands-free, portable prep with VolTRAX
‘Our method yielded over a thousand high quality, full-length draft virus genome sequences that could not be fully recovered using short read assembly approaches applied to the same samples’
Field-forward sequencing with Oxford Nanopore technology: a strategy to establish the upside-down mangrove jellyfish Cassiopea xamachana as a bioindicator
- Analyse samples at source using powerful, portable MinION devices
- Get rapid access to results with 10-minute sample prep, real-time data streaming, and local- or cloud-based analysis pipelines
- Interrogate microbiomes using rapid metagenomic, 16S, and/or RNA sequencing workflows
- Generate complete, closed microbial genomes from metagenomics samples using long nanopore sequencing reads
- Differentiate between closely related species and resolve repetitive regions
‘Our analysis demonstrated that the MinION has the ability to provide rRNA operon sequence data of sufficient quality for characterising the microbiota of complex environmental samples and provided results that are reproducible, quantitative, and consistent’
MinION sequencing from the extreme to the everyday
- Sequence samples at point of collection using MinION devices
- Generate results in real time — in the lab or field
- Comprehensively characterise genomes — including repeats, SNVs, SVs – using long and ultra-long sequencing reads
- Streamline your workflow with 10-minute sample prep
- Sensitive whole genome, targeted, and RNA sequencing approaches
- Scale to suit your needs — 1.8 Gb Flongle; 30 Gb MinION; 150 Gb GridION; 4,800/9,600 Gb PromethION P24/P48
‘…nanopore sequencing can be implemented in a remote tropical forest to quickly and accurately identify species using DNA barcoding, as we generated consensus sequences for species resolution with an accuracy of >99% in less than 24 hours after collecting specimens’
Unraveling shark secrets: sequencing genomes and microbiomes for research and conservation
- Sequence samples at point of collection using MinION devices
- Generate results in real time — in the lab or field
- Comprehensively characterise pathogen genomes — including repeats, SNVs, SVs — using long and ultra-long sequencing reads
- Streamline your workflow with 10-minute sample prep
- Sensitive whole genome, targeted, and RNA sequencing approaches
- Scale to suit your needs using Flongle, MinION, GridION, or PromethION P24/P48
‘…the first reads matching porcine epidemic diarrhea virus (PEDV) and rotavirus a (RVA) were generated respectively after 7 and 24 seconds of sequencing’
Nanopore sequencing and analysis of plant pathogenic viruses: more than just rapid diagnostics?
- Identify and characterise pathogens in real time
- Sequence and analyse at sample source using portable MinION devices
- Utilise rapid, unbiased metagenomic workflows with 10-minute library prep
- Access local or cloud-based analysis pipelines
- Scale to suit your needs using Flongle, MinION, GridION, or PromethION P24/P48
‘MinION sequencing holds promise as a practical, front-line strategy for on-site pathogen characterization to speed the public health response during a biothreat emergency’
Deployable NGS for Influenza virus field surveillance and outbreak response
Which kit is best for you?
