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Overview

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Read length Nanopores read the length of DNA presented to them. Longest read so far: > 2Mb.
Yield per flow cell, DNA/cDNA (yields are also dependent on chosen sample and preparation methods. BIF-best in field) 2 Gb (BIF) 50 Gb 50 Gb 50 Gb 220 Gb 220 Gb
Number of flow cells per device 1 1 1 5 24 48
Yield per device (yields are also dependent on chosen sample and preparation method). Up to: 2Gb 50 Gb 50 Gb 250 Gb 5.2 Tb 10.5 Tb
System access From $1,440 From $1,000 From $4,990 From $49,995 From $195,455 From $327,455
Suitable applications include Amplicons
Panels/targeted sequencing
Quality testing
Small sequencing tests
Whole genomes/exomes
Metagenomics
Targeted sequencing
Whole transcriptome (cDNA)
Smaller transcriptomes (direct RNA)
Multiplexing for smaller samples
Whole genomes/exomes
Metagenomics
Targeted sequencing
Whole transcriptome (cDNA)
Smaller transcriptomes (direct RNA)
Multiplexing for smaller samples
Particularly suitable for field use
Larger genomes or projects
Whole transcriptomes (direct RNA or cDNA)
Large numbers of samples
Very large genomes or projects
Population-scale human
Whole transcriptomes
Very large numbers of samples
Availability Buy now Buy now Buy now Buy now Buy now Buy now
 

Purchasing information

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
System Price Starter Pack $1,440 $1,000 $4,990 $49,995 $195,455 $327,455
Number of flow cells included 12 1 (plus voucher for second free flow cell) 6 60 120 240
Number of Library preparation kits included - 1 1 10 20 40
Software Licence and warranty included - - 12 months 12 months 12 months 12 months
System Price CapEx - - $6,990 $69,455 $295,455 $490,455
Number of flow cells included FOC with CapEx - - - - - -
Number of Library preparation kits included FOC with CapEx - - - - - -
Software Licence and warranty included in CapEx - - 12 months 12 months 12 months 12 months
Software Licence and Warranty annual charge - - $300 $12,500 $20,000 $30,000
 

Output and price per Gb

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Number of channels per flow cell 126 512 512 512 3000 3000
Number of flow cells per device 1 1 1 5 24 48
Price per flow cell $90 $900 - $475 $900 - $475 $900 - $475 $2000 - $625 $2000 - $625
Run time 1 min - 16 hours 1 min - 48 hours 1 min - 48 hours 1 min - 48 hours 1 min - 72 hours 1 min - 72 hours
DNA sequencing yield per flow cell
(typical in field - best in field (BIF). Yields are also dependent on chosen sample and preparation methods)
2Gb BIF 15 - 30 Gb 15 - 30 Gb 15 - 30 Gb 100 - 180 Gb 100 - 180 Gb
DNA sequencing yield per device
(typical in field - best in field)
1 - 2 Gb 15 - 30 Gb 15 - 30 Gb 75 - 150 Gb 2.4 - 4.3 Tb 4.8 - 8.6 Tb
Price per Gb, DNA sequencing  @1 - 2 Gb
$90 per flow cell: 90 - 45
@15 - 30 Gb
$900 per flow cell: $60 - 30
$790 per flow cell: $53 - 26
$675 per flow cell: $45 - 23
$500 per flow cell: $33 - 16
$475 per flow cell: $31 - 15
@15 - 30 Gb
$900 per flow cell: $60 - 30
$790 per flow cell: $53 - 26
$675 per flow cell: $45 - 23
$500 per flow cell: $33 - 16
$475 per flow cell: $31 - 15
@75 - 150 Gb
$900 per flow cell: $60 - 30
$790 per flow cell: $53 - 26
$675 per flow cell: $45 - 23
$500 per flow cell: $33 - 16
$475 per flow cell: $31 - 15
@100 - 300 Gb
$1600 per flow cell: $16-5
$940 per flow cell: $9-3.1
$680 per flow cell: $7-2.3
$625 per flow cell: $6-2
@100 - 300 Gb
$1600 per flow cell: $16-5
$940 per flow cell: $9-3.1
$680 per flow cell: $7-2.3
$625 per flow cell: $6-2
 

Sample Preparation

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Starting Material DNA, amplicons, cDNA, Direct RNA
Sample input per library Protocols from 10 pg - 1 ug
Sample Preparation time Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available
Library Preparation cost per run $25 - $50 $99 $99 $99 $99 $99
Multiplex options Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96

PCR Barcodes 1 - 96
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
 

Specifications

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Power requirement Powered by MinION / GridION Powered by Laptop / MinIT 25 W 800 W 2 kW 2 kW
Dimensions W 105, H 23, D 8 mm W 105, H 23, D 33 mm W 140, H 30, D 116 mm W 365, H 220, D 360 mm Sequencer: W 590 H 190 D 430 mm;
Data Acquisition unit W 178 H 440 D 470 mm
Sequencer: W 590 H 190 D 430 mm;
Data Acquisition unit W 178 H 440 D 470 mm
Weight 20 g 87 g 450 g 11 Kg Sequencer: 28 Kg
Data Acquisition unit: 25 Kg
Sequencer: 28 Kg
Data Acquisition unit: 25 Kg
Data Transfer - - Ethernet/MicroSD/USB/GSM/Wifi 1 Gb Ethernet Dual 10 Gb Fibre Dual 10 Gb Fibre
Storage - - 1 TB SSD 4 TB SSD 32 TB SSD 64 TB SSD
RAM - - 8 GB 64 GB 384 GB 384 GB
 

Platform

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Read length Nanopores read the entire length of the fragment of DNA/RNA presented to them. Longest read so far: > 2Mb.  Accuracy is maintained throughout the fragment.
Single Molecule Accuracy R9: Modal >97% Accuracy. R10: Modal >97% Accuracy.
Consensus Accuracy R9.4.1: Current best Q45 (>99.99%)
R10: Current Best Q50 (99.999%)
Sample Preparation time Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available
Modified base detection Yes - Base modification information available in raw signal and users can access information with additional analysis tools
Time to 1st usable data 2 minutes
Analysis tools Community developed, Tutorials, EPI2ME. More information
             
 

Application

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Large whole genome Sequencing - - Yes Yes Yes Yes
Small whole genome Sequencing Yes Yes: Low to medium plex Yes: Low to medium plex Yes: Low to medium plex Yes: Highly multiplexed Yes: Highly multiplexed
Targeted Sequencing Yes: Low to medium plex Yes: Medium to high plex Yes: Medium to high plex Yes: Medium to high plex Yes: Highly multiplexed Yes: Highly multiplexed
Metagenomics Yes: Species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID
RNA Sequencing Yes: Isoform Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment
Epigenetics Yes Yes Yes Yes Yes Yes
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