Overview
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| Read length | Nanopores read the length of DNA presented to them. Longest read so far sequencied in > 2Mb. | |||||
| Yield per flow cell (Best Internal) | 2 Gb | 50 Gb | 50 Gb | 50 Gb | 220 Gb | 220 Gb |
| Number of flow cells per device | 1 | 1 | 1 | 5 | 24 | 48 |
| Yield per device | 50 Gb | 50 Gb | 250 Gb | 5.2 Tb | 10.5 Tb | |
| System access | From $1,760 | From $1,000 | From $4,900 | From $49,995 | From $165,000 | From $285,000 |
| Application | Amplicons Panels/targeted sequencing Quality testing Amall sequencing tests |
Whole genomes/exomes Metagenomics Targeted sequencing Whole transcriptome (cDNA) Smaller transcriptomes (direct RNA) Multiplexing for smaller samples |
Whole genomes/exomes Metagenomics Targeted sequencing Whole transcriptome (cDNA) Smaller transcriptomes (direct RNA) Multiplexing for smaller samples Particularly suitable for field use |
Larger genomes or projects Whole transcriptomes (direct RNA or cDNA) Large numbers of samples |
Very large genomes or projects Population-scale human Whole transcriptomes Very large numbers of samples |
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| Availability | Buy now | Buy now | Buy now | Buy now | Buy now | Buy now |
Purchasing Information
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| System Price Starter Pack | $1,760 | $1,000 | $4,900 | $49,995 | $165,000 | $285,000 |
| Number of flow cells included | 12 | 2 | 6 | 60 | 60 | 120 |
| Number of Library preparation kits included | 1 | 1 | 1 | 10 | 14 | 30 |
| Software Licence and warranty included | - | - | 12 months | 4 months | 4 months | 4 months |
| System Price CapEx | - | - | $9,995 | $95,455 | $297,000 | $595,000 |
| Number of flow cells included FOC with CapEx | - | - | 8 | 60 | 60 | 120 |
| Number of Library preparation kits included FOC with CapEx | 1 | 1 | 1 | 10 | 14 | 30 |
| Software Licence and warranty included in CapEx | - | - | 3 years | 3 years | 3 years | 3 years |
| Software Licence and Warranty annual charge | - | - | $300 | $12,500 | $20,000 | $30,000 |
Output and price per Gb
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| Number of channels per flow cell | 126 | 512 | 512 | 512 | 3000 | 3000 |
| Number of flow cells per device | 1 | 1 | 1 | 5 | 24 | 48 |
| Price per flow cell | $90 | $900 - $475 | $900 - $475 | $900 - $475 | $2000 - $625 | $2000 - $625 |
| Run time | 1 min - 16 hours | 1 min - 48 hours | 1 min - 48 hours | 1 min - 48 hours | 1 min - 72 hours | 1 min - 72 hours |
| Yield per flow cell (typical in field - best in field) |
1 - 2 Gb | 15 - 30 Gb | 15 - 30 Gb | 15 - 30 Gb | 100 - 180 Gb | 100 - 180 Gb |
| Yield per device (typical in field - best in field) |
1 - 2 Gb | 15 - 30 Gb | 15 - 30 Gb | 75 - 150 Gb | 2.4 - 4.3 Tb | 4.8 - 8.6 Tb |
| Price per Gb | @1 - 2 Gb $90 per flow cell: 90 - 45 |
@15 - 30 Gb $900 per flow cell: $60 - 30 $790 per flow cell: $53 - 26 $675 per flow cell: $45 - 23 $500 per flow cell: $33 - 16 $475 per flow cell: $31 - 15 |
@15 - 30 Gb $900 per flow cell: $60 - 30 $790 per flow cell: $53 - 26 $675 per flow cell: $45 - 23 $500 per flow cell: $33 - 16 $475 per flow cell: $31 - 15 |
@75 - 150 Gb $900 per flow cell: $60 - 30 $790 per flow cell: $53 - 26 $675 per flow cell: $45 - 23 $500 per flow cell: $33 - 16 $475 per flow cell: $31 - 15 |
@2.4 - 4.3 Tb $2000 per flow cell: $20 - 11 $1700 per flow cell: $18 - 9 $1475 per flow cell: $15 - 8 $1225 per flow cell: $12 - 7 $975 per flow cell: $10 - 5 $625 per flow cell: $6 - 3 |
@4.8 - 8.6 Tb $2000 per flow cell: $20 - 11 $1700 per flow cell: $18 - 9 $1475 per flow cell: $15 - 8 $1225 per flow cell: $12 - 7 $975 per flow cell: $10 - 5 $625 per flow cell: $6 - 3 |
Sample Preparation
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| Starting Material | DNA, amplicons, cDNA, Direct RNA | |||||
| Sample input per library | Protocols from 10 pg - 1 ug | |||||
| Sample Preparation time | Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available | |||||
| Library Preparation cost per run | $25 - $50 | $99 | $99 | $99 | $99 | $99 |
| Multiplex options | Rapid Barcodes: 1 - 12 Native Barcodes: 1 - 24 PCR Barcodes 1 - 96 |
Rapid Barcodes: 1 - 12 Native Barcodes: 1 - 24 PCR Barcodes 1 - 96 Combinatorial Barcodes > 2K combinations possible |
Rapid Barcodes: 1 - 12 Native Barcodes: 1 - 24 PCR Barcodes 1 - 96 Combinatorial Barcodes > 2K combinations possible |
Rapid Barcodes: 1 - 12 Native Barcodes: 1 - 24 PCR Barcodes 1 - 96 Combinatorial Barcodes > 2K combinations possible |
Rapid Barcodes: 1 - 12 Native Barcodes: 1 - 24 PCR Barcodes 1 - 96 Combinatorial Barcodes > 2K combinations possible |
Rapid Barcodes: 1 - 12 Native Barcodes: 1 - 24 PCR Barcodes 1 - 96 Combinatorial Barcodes > 2K combinations possible |
Specifications
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| Power requirement | Powered by MinION / GridION | Powered by Laptop / MinIT | 25 W | 800 W | 2 kW | 2 kW |
| Dimensions | W 105, H 23, D 8 mm | W 105, H 23, D 33 mm | W 140, H 30, D 116 mm | W 365, H 220, D 360 mm | Sequencer: W 590 H 190 D 430 mm; Data Acquisition unit W 178 H 440 D 470 mm |
Sequencer: W 590 H 190 D 430 mm; Data Acquisition unit W 178 H 440 D 470 mm |
| Weight | 20 g | 87 g | 450 g | 11 Kg | Sequencer: 28 Kg Data Acquisition unit: 25 Kg |
Sequencer: 28 Kg Data Acquisition unit: 25 Kg |
| Data Transfer | - | - | Ethernet/MicroSD/USB/GSM/Wifi | 1 Gb Ethernet | Dual 10 Gb Fibre | Dual 10 Gb Fibre |
| Storage | - | - | 1 TB SSD | 4 TB SSD | 32 TB SSD | 64 TB SSD |
| RAM | - | - | 8 GB | 64 GB | 384 GB | 384 GB |
Platform
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| Read length | Nanopores read the length of DNA presented to them. Longest read so far sequencied in > 2Mb. | |||||
| Single Molecule Accuracy | R9: Modal 95% Accuracy. R10: Modal 94% Accuracy | |||||
| Sample Preparation time | Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available | |||||
| Consensus Accuracy | R9.4.1: Current best Q44 R10: Current Best Q50 |
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| Modified base detection | Yes - Base modification information available in raw signal and users can access information with additional analysis tools | |||||
| Time to 1st usable data | 2 minutes | |||||
| Analysis tools | Community developed, Tutorials, EPI2ME | |||||
Application
| Flongle | MinION Mk1B | MinION Mk1C | GridION X5 | PromethION 24 | PromethION 48 | |
|---|---|---|---|---|---|---|
| Large whole genome Sequencing | - | - | Yes | Yes | Yes | Yes |
| Small whole genome Sequencing | Yes | Yes: Low to medium plex | Yes: Low to medium plex | Yes: Low to medium plex | Yes: Highly multiplexed | Yes: Highly multiplexed |
| Targeted Sequencing | Yes: Low to medium plex | Yes: Medium to high plex | Yes: Medium to high plex | Yes: Medium to high plex | Yes: Highly multiplexed | Yes: Highly multiplexed |
| Metagenomics | Yes: Species ID | Yes: Quantitative species ID | Yes: Quantitative species ID | Yes: Quantitative species ID | Yes: Quantitative species ID | Yes: Quantitative species ID |
| RNA Sequencing | Yes: Isoform | Yes: Isoform & expression from same experiment | Yes: Isoform & expression from same experiment | Yes: Isoform & expression from same experiment | Yes: Isoform & expression from same experiment | Yes: Isoform & expression from same experiment |
| Epigenetics | Yes | Yes | Yes | Yes | Yes | Yes |