Which nanopore device is best for you?
Long read, direct DNA/RNA/epigenetic sequencing, scalable, real time/rapid, on-demand sequencing that is easy to use and install.
- Your portable device for smaller, individual, rapid tests.
- When you don’t want to multiplex samples or start a larger run.
- Amplicons, panels/targeted sequencing, quality testing and more.
- For use with MinIT or a laptop.
- Your personal sequencer, putting you in control.
- Whether in your lab or out in the field.
- Whole genomes/exomes, metagenomics, targeted sequencing, whole transcriptome (cDNA), smaller transcriptomes (direct RNA), multiplexing for smaller samples and more.
- For use with MinIT or a laptop.
- High throughput sequencing, in modular form (up to 5 flow cells) to be on-demand.
- For your lab or to offer as a service.
- Larger genomes or projects, whole transcriptomes (direct RNA or cDNA) or where you have larger numbers of samples and more.
- Compute included for real time data analysis and easy installation.
- Very high throughput sequencing, in modular form (up to 48 flow cells) to be on-demand.
- For your lab or as a service.
- Larger genomes or projects, whole transcriptomes (direct RNA or cDNA), very large numbers of samples and more.
- Compute included for real time data analysis and easy installation.
Full comparison table
| Flongle | MinION Mk 1B | GridION X5 | PromethION (1 flow cell) |
PromethION (48 flow cells) |
|
|---|---|---|---|---|---|
| Read length | fragment length = read length. Longest read now >2 Mb | ||||
| Run time | 1 min - 16 hrs | 1 min - 48 hrs | 1 min - 48 hrs | 1 min - 64 hrs | 1 min - 64 hrs |
| Yield DNA sequencing | |||||
| Theoretical maximum 1D Yield | Up to 3.3 Gb | Up to 40 Gb | Up to 200 Gb | Up to 315 Gb | Up to 15 Tb |
| Current yield range 1D | Early access to start ASAP - commercial target 1 Gb | Up to 30Gb (Rev D Chip) | Up to 150Gb (RevD Chip) | Up to 150 Gb | |
| Cost per Gb: (cited as cost per Gb of data at a certain yield per flow cell, best package price) | @1Gb: $90 | @10Gb: $50 @20Gb: $25 @30Gb: $16.50 |
OpEx: @10Gb: $47.50 @20Gb: $23.75 @30Gb: $15.70 CapEx: @10Gb: $30 @20Gb $15 @30Gb: $10.00 |
@50Gb: $14 @100Gb: $7 @150Gb: $3.5 |
@50Gb: $12.5 @100Gb: $6.25 @150Gb: $4.1 |
| Starter pack fee (other purchasing schemes also available) |
Provided as part of early access pack | No instrument cost. Starter kit (includes MinION, 2 flow cells & sequencing kit) $1000 | $50k | No instrument cost | No instrument cost |
| Multiplexing enabled | Not in first release | Kits for 96 samples | Kits for 96 samples | Coming soon | Coming soon |
| Number of channels available for sequencing | Up to 126 | Up to 512 | up to 2,560 | Up to 3,000 | Up to 144,000 |
Additional Information
| Flongle | MinION Mk 1B | GridION X5 | PromethION (1 flow cell) |
PromethION (48 flow cells) |
|
|---|---|---|---|---|---|
| Price | |||||
| Annual service contract2 | None | None | $15k Support | $20k (compute module support) | $20k (compute module support) |
| Reagent cost per run3 | ~$20 | $99 | $99 | $99 | $99 |
| Flow cell cost (depending on order type and volume)3 | ~$90 | $500 - $900 | $299 - $900 | $625 - $2000 | $625 - $2000 |
| Your sample | |||||
| Sample input requirement 4 | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg |
| Flow cell input volume5 | 30 µl | 75 µl | 75 µl | 35 µl per sample well (4 wells in a flow cell) | 35 µl per sample well (192 wells in a PromethION) |
| Minimum sample preparation time | 10 mins | 10 mins | 10 mins | 10 mins | 10 mins |
| System opearation | |||||
| Time to first usable read (data available in real time) | 2 mins | 2 mins | 2 mins | 2 mins | 2 mins |
| Raw read accuracy6 | Up to 99% | Up to 99% | Up to 99% | Up to 99% | Up to 99% |
| Raw data available | Yes | Yes | Yes | Yes | Yes |
| Modified base detection | Yes | Yes | Yes | Yes | Yes |
| Data analysis | Local offline/MinIT | Local offline/MinIT | On Unit | On Unit | On Unit |
| System specifications | |||||
| Power requirement | Powered by MinION | USB 3 (1 watt) | 600 W | ~2 kW | ~2 kW |
| Potential raw data requirements (Bytes per second) | Up to 2 MBps | Up to 5 MBps | Up to 25 MBps | Up to 30 MBps | Up to 1.44 GBps |
| Dimensions | Fits into MinION | W 105, H 23, D 33 mm | W 365 H 220 D 360 mm | W 440, H 400, D 240 mm | W 440, H 400, D 240 mm |
| Weight | Same as MinION | 87 g | 11 Kg | 40 Kg | 40 Kg |
| Facility modifications required | None | None | None | None | None |
- Data is available within a min of the run starting. If a user reaches the answer necessary quickly they can simply stop the run, wash, load and analyse another sample. Current scripts are set to run for 48 hrs but these can be stopped and started as necessary.
- Upgrades or replacements will be made available promptly. Customers may propose alternative service arrangements and bespoke contracts.
- Some PromethION packs may be subject to availability.
- Protocols with and without amplification are available. Lowest amplification free input provided with a protocol is 200ng. The system will detect lower inputs without amplificatio but will not provide the optimal throughput.
- Higher sample input will result in a higher yield. The system can, in principle, detect just one molecule subject to a long enough wait and random diffusion.
- Accuracy may depend on base calling software chosen, user chosen run settings and other factors. Future versions of the system may exceed figures shown here.