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Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Overview

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Read length Nanopores read the length of DNA presented to them. Longest read so far: > 4 Mb.
Number of flow cells per device 1 1 1 5 24 48
Best in field yield per flow cell (yields will vary according to sample and preparation methods) 1.8 Gb 42 Gb 42 Gb 42 Gb 245 Gb 245 Gb
System access From $1,460 From $1,000 From $4,900 From $49,995 From $195,455 From $285,455
Availability Buy now Buy now Buy now Buy now Buy now Buy now
 

Purchasing information

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
System Price Starter Pack $1,460 $1,000 $4,900 $49,995 $195,455 $285,455
Number of flow cells included 12 1 (plus voucher for second free flow cell) 6 60 120 192
Number of Library preparation kits included - 1 1 10 20 32
Software Licence and warranty included - - 12 months 12 months 12 months 12 months
System Price CapEx - - $9,300 $69,955 $335,455 $530,000
Software Licence and warranty included in CapEx - - 12 months 12 months 12 months 12 months
Software Licence and Warranty annual charge - - $300 $12,500 $20,000 $30,000
 

Output and price per Gb

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Number of channels per flow cell 126 512 512 512 3000 3000
Number of flow cells per device 1 1 1 5 24 48
Price per flow cell $90 $900 - $475 $900 - $475 $900 - $475 $2000 - $625 $2000 - $625
Run time 1 min - 16 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours
Yields in field are dependent on sample and preparation methods. Users can get outputs in the following ranges per flow cell when utilising the latest chemistries and protocols 1 - 2 Gb 10 - 30 - 50 Gb 10 - 30 - 50 Gb 10 - 30 - 50 Gb 100 - 200 - 300 Gb 100 - 200 - 300 Gb
Price per Gb for different flow cell yields (yields vary according to sample and preparation methods) @1 - 2 Gb
$90 per flow cell:
$90 - 45
@10 - 30 - 50 Gb
$900 per flow cell:
$90 - 30 - 18
$790 per flow cell:
$79 - 26 - 16
$675 per flow cell:
$68 - 23 - 14
$500 per flow cell:
$50 - 17 - 10
$475 per flow cell:
$48 - 16 - 9.5
@10 - 30 - 50 Gb
$900 per flow cell:
$90 - 30 - 18
$790 per flow cell:
$79 - 26 - 16
$675 per flow cell:
$68 - 23 - 14
$500 per flow cell:
$50 - 17 - 10
$475 per flow cell:
$48 - 16 - 9.5
@10 - 30 - 50 Gb
$900 per flow cell:
$90 - 30 - 18
$790 per flow cell:
$79 - 26 - 16
$675 per flow cell:
$68 - 23 - 14
$500 per flow cell:
$50 - 17 - 10
$475 per flow cell:
$48 - 16 - 9.5
@100 - 200 - 300 Gb
$1,600 per flow cell:
$16 - 8 - 5
$1,120 per flow cell:
$11 - 6 - 4
$940 per flow cell:
$9 - 5 - 3.1
$680 per flow cell:
$7 - 3.4 - 2.3
$625 per flow cell:
$6 - 3 - 2
@100 - 200 - 300 Gb
$1,600 per flow cell:
$16 - 8 - 5
$1,120 per flow cell:
$11 - 6 - 4
$940 per flow cell:
$9 - 5 - 3.1
$680 per flow cell:
$7 - 3.4 - 2.3
$625 per flow cell:
$6 - 3 - 2
 

Sample Preparation

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Starting Material DNA, amplicons, cDNA, Direct RNA
Sample input per library Protocols from 10 pg - 1 ug
Sample Preparation time Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available
Library Preparation cost per run $25 - $50 $99 $99 $99 $99 $99
Multiplex options Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96

PCR Barcodes 1 - 96
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
 

Specifications

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Power requirement Powered by MinION / GridION Powered by Laptop / MinIT 25 W 800 W 2 kW 2 kW
Dimensions W 105, H 23, D 8 mm W 105, H 23, D 33 mm W 140, H 30, D 116 mm W 365, H 220, D 360 mm Sequencer: W 590 H 190 D 430 mm;
Data Acquisition unit W 178 H 440 D 470 mm
Sequencer: W 590 H 190 D 430 mm;
Data Acquisition unit W 178 H 440 D 470 mm
Weight 20 g 87 g 450 g 11 Kg Sequencer: 28 Kg
Data Acquisition unit: 25 Kg
Sequencer: 28 Kg
Data Acquisition unit: 25 Kg
Data Transfer - - Ethernet/MicroSD/USB/GSM/Wifi 1 Gb Ethernet Dual 10 Gb Fibre Dual 10 Gb Fibre
Storage - - 1 TB SSD 4 TB SSD 32 TB SSD 64 TB SSD
RAM - - 8 GB 64 GB 384 GB 384 GB
 

Platform

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Read length Nanopores read the entire length of the fragment of DNA/RNA presented to them. Longest read so far: > 4Mb.  Accuracy is maintained throughout the fragment.
Single Molecule Accuracy R9 modal >98.3%, New chemistry >99% (coming soon)
Consensus Accuracy R9.4.1: Current best Q45 (>99.99%)
R10: Current Best Q50 (99.999%)
Sample Preparation time Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available
Modified base detection Yes - Base modification information available in raw signal
Time to 1st usable data 2 minutes
Analysis tools Community developed, Tutorials, EPI2ME. More information
             
 

Application

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 24 PromethION 48
Large whole genome Sequencing Library prep QC - Yes Yes Yes Yes
Small whole genome Sequencing Yes Yes: Low to medium plex Yes: Low to medium plex Yes: Low to medium plex Yes: Highly multiplexed Yes: Highly multiplexed
Targeted Sequencing Yes: Low to medium plex Yes: Medium to high plex Yes: Medium to high plex Yes: Medium to high plex Yes: Highly multiplexed Yes: Highly multiplexed
Metagenomics Yes: Species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID
RNA Sequencing Yes: Isoform Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment
Epigenetics Yes Yes Yes Yes Yes Yes
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