Which nanopore device is best for you?
Long read, direct DNA/RNA/epigenetic sequencing, scalable, real time/rapid, on-demand sequencing that is easy to use and install.
- Your portable device for smaller, individual, rapid tests.
- When you don’t want to multiplex samples or start a larger run.
- Amplicons, panels/targeted sequencing, quality testing and more.
- For use with MinIT or a laptop.
- Your personal sequencer, putting you in control.
- Whether in your lab or out in the field.
- Whole genomes/exomes, metagenomics, targeted sequencing, whole transcriptome (cDNA), smaller transcriptomes (direct RNA), multiplexing for smaller samples and more.
- For use with MinIT or a laptop.
- High throughput sequencing, in modular form (up to 5 flow cells) to be on-demand.
- For your lab or to offer as a service.
- Larger genomes or projects, whole transcriptomes (direct RNA or cDNA) or where you have larger numbers of samples and more.
- Compute included for real time data analysis and easy installation.
- Very high throughput sequencing, in modular form (up to 48 flow cells) to be on-demand.
- For your lab or as a service.
- Larger genomes or projects, whole transcriptomes (direct RNA or cDNA), very large numbers of samples and more.
- Compute included for real time data analysis and easy installation.
Spec comparison table
| Flongle | MinION Mk 1B | GridION X5 | PromethION (1 flow cell) |
PromethION P24/P48 (24 or 48 flow cells) |
|
|---|---|---|---|---|---|
| Read length | fragment length = read length. Longest read now >2 Mb | ||||
| Run time | 1 min - 24 hrs | 1 min - 48 hrs | 1 min - 48 hrs | 1 min - 64 hrs | 1 min - 64 hrs |
| Yield DNA sequencing | |||||
| Theoretical maximum 1D Yield | Up to 3.3 Gb (1 flow cell) | Up to 50 Gb (1 flow cell) | Up to 250 Gb (1 flow cell) | Up to 315 Gb | Up to 315 Gb |
| Current yield range 1D (customer best, January 2019) | up to 1.8Gb | Up to 30Gb (Rev D Chip) | Up to 150Gb (RevD Chip) | Up to 160 Gb | Up to 7.6 Tb |
| Cost per Gb: (cited as cost per Gb of data at a certain yield per flow cell, best package price) | @1Gb: $90 | @10Gb: $47.50 @20Gb: $23.75 @30Gb: $15.70 |
@10Gb: $47.50 @20Gb: $23.75 @30Gb: $15.70 |
@50Gb: $14 @100Gb: $7 @150Gb: $3.5 |
|
| Starter pack cost | $5,250 | No instrument cost. Basic package (MinION, 2 flow cells & 1 sequencing kit) $1000 | $49,995 (No instrument cost) | 24/48: $165,000/$285,000. (No instrument cost) | 24/48: $165,000/$285,000. (No instrument cost) |
| Other purchasing schemes | Coming soon | No instrument cost. Enhanced (MinION, 8 flow cells & 2 sequencing kits) $4,500 Project (MinION, 14 flow cells & 3 sequencing kits) $12,700 |
CapEx $95,455 (No instrument cost) | CapEx 24/48: $297,000/$595,000 (No instrument cost) | CapEx 24/48: $297,000/$595,000 (No instrument cost) |
| Multiplexing enabled | Kits for 96 samples | Kits for 96 samples | Kits for 96 samples | Kits for 24 samples | Kits for 24 samples |
| Number of channels available for sequencing | Up to 126 | Up to 512 | up to 2,560 | Up to 3,000 | Up to 144,000 |
Additional Information
| Flongle | MinION Mk 1B | GridION X5 | PromethION (1 flow cell) |
PromethION P24/P48 (24 or 48 flow cells) |
|
|---|---|---|---|---|---|
| Price | |||||
| Software license and device warranty2 | None | None | 4 months (with Starter Pack) or 3 years (CapEx) | 4 months (with Starter Pack) or 3 years (CapEx) | 4 months (with Starter Pack) or 3 years (CapEx) |
| Reagent cost per run3 | ~$20 | $99 | $99 | $99 | $99 |
| Flow cell cost (depending on order type and volume)3 | ~$90 | $475 - $900 | $475 - $900 | $625 - $2000 | $625 - $2000 |
| Your sample | |||||
| Sample input requirement per flow cell 4 | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg |
| Flow cell input volume5 | 30 µl | 75 µl | 75 µl | 150 µl | 150 µl |
| Minimum sample preparation time | 10 mins | 10 mins | 10 mins | 10 mins | 10 mins |
| System opearation | |||||
| Time to first usable read (data available in real time) | 2 mins | 2 mins | 2 mins | 2 mins | 2 mins |
| Raw read accuracy6 | Up to 99% | Up to 99% | Up to 99% | Up to 99% | Up to 99% |
| Raw data available | Yes | Yes | Yes | Yes | Yes |
| Modified base detection | Yes | Yes | Yes | Yes | Yes |
| Data analysis | Local offline/MinIT | Local offline/MinIT | On Unit | On Unit | On Unit |
| System specifications | |||||
| Power requirement | Powered by MinION | USB 3 (1 watt) | 600 W | ~2 kW | ~2 kW |
| Potential raw data requirements (Bytes per second) | Up to 2 MBps | Up to 5 MBps | Up to 25 MBps | Up to 30 MBps | Up to 1.44 GBps |
| Dimensions | W 105, H 23, D 8 mm | W 105, H 23, D 33 mm | W 365 H 220 D 360 mm | W 590 H 190 D 430 mm | |
| Weight | 20 g | 87 g | 11 kg | 28 kg (sequencing unit) 25 kg (data acquisition unit) | |
| Facility modifications required | None | None | None | None | None |
- Data is available within a min of the run starting. If a user reaches the answer necessary quickly they can simply stop the run, wash, load and analyse another sample. Current scripts are set to run for 48 hrs but these can be stopped and started as necessary.
- Upgrades or replacements will be made available promptly. Customers may propose alternative service arrangements and bespoke contracts.
- Some PromethION packs may be subject to availability.
- Protocols with and without amplification are available. Lowest amplification free input provided with a protocol is 400ng. The system will detect lower inputs without amplification but will not provide the optimal throughput.
- Higher sample input will result in a higher yield. The system can, in principle, detect just one molecule subject to a long enough wait and random diffusion.
- Accuracy may depend on base calling software chosen, user chosen run settings and other factors. Future versions of the system may exceed figures shown here.