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Rapid sequencing DNA - PCR Barcoding Kit 24 V14 (SQK-RPB114.24) (RPB_9191_v114_revG_07May2025)

DateVersionLanguageDeviceChanges
5/29/2025RPB_9191_v114_revG_07May2025EnglishFlongle
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/29/2025RPB_9191_v114_revG_07May2025EnglishGridION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/29/2025RPB_9191_v114_revG_07May2025EnglishMinION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/29/2025RPB_9191_v114_revG_07May2025EnglishPromethION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishFlongle
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishGridION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishMinION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RPB_9191_v114_revG_07May2025EnglishPromethION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishFlongle
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishGridION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishMinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RPB_9191_v114_revF_11Dec2024EnglishPromethION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RPB_9191_v114_revE_11Dec2024EnglishPromethION
11th December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol" and links to MinION Mk1B/Mk1D user manuals in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link to EPI2ME in "Downstream analysis", "EPI2ME workflows". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishFlongle
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishGridION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishMinION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
10/16/2024RPB_9191_v114_revD_16Oct2024EnglishPromethION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishFlongle
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishGridION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishMinION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/17/2024RPB_9191_v114_revC_17Sep2024EnglishPromethION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishFlongle
19th September 2023 - Updated flow cell loading instructions to include light shield installation
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishGridION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishMinION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
9/19/2023RPB_9191_v114_revB_28Jun2023EnglishPromethION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishFlongle Download -
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishPromethion Download -
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishGridion Download -
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishMinion Download -

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