Rapid sequencing gDNA - barcoding (SQK-RBK004) (RBK_9054_v2_revAF_14Aug2019)


DateVersionLanguageDeviceChanges
9/7/2023RBK_9054_v2_revAF_14Aug2019EnglishFlongle
12th July 2023 - Updated product legacy availability
9/7/2023RBK_9054_v2_revAF_14Aug2019EnglishGridION
12th July 2023 - Updated product legacy availability
9/7/2023RBK_9054_v2_revAF_14Aug2019EnglishMinION
12th July 2023 - Updated product legacy availability
March 10th, 2023RBK_9054_v2_revAE_14Aug2019EnglishFlongle Download February 2023 - Updated product phase to 'Legacy'
March 10th, 2023RBK_9054_v2_revAE_14Aug2019EnglishGridion Download February 2023 - Updated product phase to 'Legacy'
March 10th, 2023RBK_9054_v2_revAE_14Aug2019EnglishMinion Download February 2023 - Updated product phase to 'Legacy'
January 11th, 2023RBK_9054_v2_revAD_14Aug2019EnglishFlongle Download January 2023 - Updated kit Flongle Flow Cell Priming kit name to Flongle Sequencing Expansion (EXP-FSE001) across protocol
January 11th, 2023RBK_9054_v2_revAD_14Aug2019EnglishGridion Download January 2023 - Updated kit Flongle Flow Cell Priming kit name to Flongle Sequencing Expansion (EXP-FSE001) across protocol
January 11th, 2023RBK_9054_v2_revAD_14Aug2019EnglishMinion Download January 2023 - Updated kit Flongle Flow Cell Priming kit name to Flongle Sequencing Expansion (EXP-FSE001) across protocol
August 10th, 2022RBK_9054_v2_revAC_14Aug2019EnglishFlongle Download 9th August 2022 - Added information about the RAP Top-Up kit in the Equipment and Consumables section as well as splitting libraries in the Library Prep section.
August 10th, 2022RBK_9054_v2_revAC_14Aug2019EnglishGridion Download 9th August 2022 - Added information about the RAP Top-Up kit in the Equipment and Consumables section as well as splitting libraries in the Library Prep section.
August 10th, 2022RBK_9054_v2_revAC_14Aug2019EnglishMinion Download 9th August 2022 - Added information about the RAP Top-Up kit in the Equipment and Consumables section as well as splitting libraries in the Library Prep section.
July 4th, 2022RBK_9054_v2_revAB_14Aug2019EnglishFlongle Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022RBK_9054_v2_revAB_14Aug2019EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022RBK_9054_v2_revAB_14Aug2019EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 20th, 2022RBK_9054_v2_revAA_14Aug2019EnglishFlongle Download 14th April 2022 Protocol name change
April 20th, 2022RBK_9054_v2_revAA_14Aug2019EnglishGridion Download 14th April 2022 Protocol name change
April 20th, 2022RBK_9054_v2_revAA_14Aug2019EnglishMinion Download 14th April 2022 Protocol name change
April 20th, 2022RBK_9054_v2_revAA_14Aug2019EnglishAny Download 14th April 2022 Protocol name change
March 28th, 2022RBK_9054_v2_revZ_14Aug2019EnglishFlongle Download 28th March 2022 In "Equipment an consumables", the missing barcode sequences for barcodes 11 and 12 have been added.
March 28th, 2022RBK_9054_v2_revZ_14Aug2019EnglishGridion Download 28th March 2022 In "Equipment an consumables", the missing barcode sequences for barcodes 11 and 12 have been added.
March 28th, 2022RBK_9054_v2_revZ_14Aug2019EnglishMinion Download 28th March 2022 In "Equipment an consumables", the missing barcode sequences for barcodes 11 and 12 have been added.
February 11th, 2022RBK_9054_v2_revY_14Aug2019EnglishFlongle Download 11th February 2022 The library preparation step regarding pooling the samples have been updated to a simpler version.
February 11th, 2022RBK_9054_v2_revY_14Aug2019EnglishGridion Download 11th February 2022 The library preparation step regarding pooling the samples have been updated to a simpler version.
February 11th, 2022RBK_9054_v2_revY_14Aug2019EnglishMinion Download 11th February 2022 The library preparation step regarding pooling the samples have been updated to a simpler version.
December 22nd, 2021RBK_9054_v2_revX_14Aug2019EnglishFlongle Download 22nd December 2021 In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
December 22nd, 2021RBK_9054_v2_revX_14Aug2019EnglishGridion Download 22nd December 2021 In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
December 22nd, 2021RBK_9054_v2_revX_14Aug2019EnglishMinion Download 22nd December 2021 In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
December 22nd, 2021RBK_9054_v2_revW_14Aug2019EnglishFlongle Download 22nd December 2021 In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
December 22nd, 2021RBK_9054_v2_revW_14Aug2019EnglishGridion Download 22nd December 2021 In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
December 22nd, 2021RBK_9054_v2_revW_14Aug2019EnglishMinion Download 22nd December 2021 In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
October 7th, 2021RBK_9054_v2_revV_14Aug2019EnglishFlongle Download 7th October 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell": - A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
October 7th, 2021RBK_9054_v2_revV_14Aug2019EnglishGridion Download 7th October 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell": - A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
October 7th, 2021RBK_9054_v2_revV_14Aug2019EnglishMinion Download 7th October 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell": - A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
September 23rd, 2021RBK_9054_v2_revU_14Aug2019EnglishFlongle Download 23rd September 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell": - A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell." - Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
September 23rd, 2021RBK_9054_v2_revU_14Aug2019EnglishGridion Download 23rd September 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell": - A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell." - Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
September 23rd, 2021RBK_9054_v2_revU_14Aug2019EnglishMinion Download 23rd September 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell": - A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell." - Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
September 13th, 2021RBK_9054_v2_revT_14Aug2019EnglishFlongle Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021RBK_9054_v2_revT_14Aug2019EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021RBK_9054_v2_revT_14Aug2019EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
June 24th, 2021RBK_9054_v2_revS_14Aug2019EnglishFlongle Download 24th June 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
June 24th, 2021RBK_9054_v2_revS_14Aug2019EnglishGridion Download 24th June 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
June 24th, 2021RBK_9054_v2_revS_14Aug2019EnglishMinion Download 24th June 2021 In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
April 21st, 2021RBK_9054_v2_revR_14Aug2019EnglishFlongle Download 21st April 2021 In the Flongle branch of the protocol: - "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic - "Library preparation" step: the AMPure XP bead clean-up is no longer optional - "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl." - "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."
April 21st, 2021RBK_9054_v2_revR_14Aug2019EnglishGridion Download 21st April 2021 In the Flongle branch of the protocol: - "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic - "Library preparation" step: the AMPure XP bead clean-up is no longer optional - "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl." - "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."
April 21st, 2021RBK_9054_v2_revR_14Aug2019EnglishMinion Download 21st April 2021 In the Flongle branch of the protocol: - "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic - "Library preparation" step: the AMPure XP bead clean-up is no longer optional - "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl." - "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."
March 15th, 2021RBK_9054_v2_revQ_14Aug2019EnglishFlongle Download 2nd February 2021 In the Flongle branch of the protocol: - "Equipment and consumables" step: updated Flongle Sequencing Expansion (EXP-FSE001) kit contents - "Loading the Flongle flow cell" step: updated instructions. SBII, LBII and FB should be used from the Flongle Sequencing Expansion instead of SQB, LB and FB from the sequencing kit
March 15th, 2021RBK_9054_v2_revQ_14Aug2019EnglishGridion Download 2nd February 2021 In the Flongle branch of the protocol: - "Equipment and consumables" step: updated Flongle Sequencing Expansion (EXP-FSE001) kit contents - "Loading the Flongle flow cell" step: updated instructions. SBII, LBII and FB should be used from the Flongle Sequencing Expansion instead of SQB, LB and FB from the sequencing kit
March 15th, 2021RBK_9054_v2_revQ_14Aug2019EnglishMinion Download 2nd February 2021 In the Flongle branch of the protocol: - "Equipment and consumables" step: updated Flongle Sequencing Expansion (EXP-FSE001) kit contents - "Loading the Flongle flow cell" step: updated instructions. SBII, LBII and FB should be used from the Flongle Sequencing Expansion instead of SQB, LB and FB from the sequencing kit
January 20th, 2021RBK_9054_v2_revP_14Aug2019EnglishFlongle Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021RBK_9054_v2_revP_14Aug2019EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021RBK_9054_v2_revP_14Aug2019EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
December 3rd, 2020RBK_9054_v2_revO_14Aug2019EnglishFlongle Download 3rd December 2020 Updated the "Check your flow cell" section: Minimum number of active pores covered by warranty for Flongle has been changed to 50.
December 3rd, 2020RBK_9054_v2_revO_14Aug2019EnglishGridion Download 3rd December 2020 Updated the "Check your flow cell" section: Minimum number of active pores covered by warranty for Flongle has been changed to 50.
December 3rd, 2020RBK_9054_v2_revO_14Aug2019EnglishMinion Download 3rd December 2020 Updated the "Check your flow cell" section: Minimum number of active pores covered by warranty for Flongle has been changed to 50.
October 2nd, 2020RBK_9054_v2_revN_14Aug2019EnglishFlongle Download 2nd October 2020 Updated Flongle equipment and flow cell loading instructions in line with the release of the Flongle Flow Cell Priming Kit (EXP-FLP003)
October 2nd, 2020RBK_9054_v2_revN_14Aug2019EnglishGridion Download 2nd October 2020 Updated Flongle equipment and flow cell loading instructions in line with the release of the Flongle Flow Cell Priming Kit (EXP-FLP003)
October 2nd, 2020RBK_9054_v2_revN_14Aug2019EnglishMinion Download 2nd October 2020 Updated Flongle equipment and flow cell loading instructions in line with the release of the Flongle Flow Cell Priming Kit (EXP-FLP003)
October 23rd, 2019RBK_9054_v2_revM_14Aug2019EnglishFlongle Download 11th October 2019 Updated the Flongle flow cell priming and loading instructions in the Flongle branch of the protocol
October 23rd, 2019RBK_9054_v2_revM_14Aug2019EnglishGridion Download 11th October 2019 Updated the Flongle flow cell priming and loading instructions in the Flongle branch of the protocol
October 23rd, 2019RBK_9054_v2_revM_14Aug2019EnglishMinion Download 11th October 2019 Updated the Flongle flow cell priming and loading instructions in the Flongle branch of the protocol
October 10th, 2019RBK_9054_v2_revL_14Aug2019EnglishFlongle Download 10th October 2019 Updated the Flongle flow cell priming and loading instructions in the Flongle branch of the protocol
October 10th, 2019RBK_9054_v2_revL_14Aug2019EnglishGridion Download 10th October 2019 Updated the Flongle flow cell priming and loading instructions in the Flongle branch of the protocol
October 10th, 2019RBK_9054_v2_revL_14Aug2019EnglishMinion Download 10th October 2019 Updated the Flongle flow cell priming and loading instructions in the Flongle branch of the protocol
September 27th, 2019RBK_9054_v2_revK_14Aug2019EnglishFlongle Download 27th September 2019 Updated and expanded Troubleshooting guide
September 27th, 2019RBK_9054_v2_revK_14Aug2019EnglishGridion Download 27th September 2019 Updated and expanded Troubleshooting guide
September 27th, 2019RBK_9054_v2_revK_14Aug2019EnglishMinion Download 27th September 2019 Updated and expanded Troubleshooting guide
August 23rd, 2019RBK_9054_v2_revJ_14Aug2019EnglishFlongle Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019RBK_9054_v2_revJ_14Aug2019EnglishGridion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019RBK_9054_v2_revJ_14Aug2019EnglishMinion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 14th, 2019RBK_9054_v2_revI_14Aug2019EnglishFlongle Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019RBK_9054_v2_revI_14Aug2019EnglishGridion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019RBK_9054_v2_revI_14Aug2019EnglishMinion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 2nd, 2019RBK_9054_v2_revH_23Jan2018English- Download 19th July 2019 Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
June 28th, 2019RBK_9054_v2_revG_23Jan2018English- Download Updated with new information for MinKNOW release v19.06: - Compression of queued reads - Pause functionality - UI improvements including: - Flow cell health at platform QC - Flow cell status on landing page - R10 flow cell running scripts - New Fast Models for RNA and DNA - PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files - Flongle hardware check for confirming Flongle adapter functions correctly
June 12th, 2019RBK_9054_v2_revF_23Jan2018English- Download Update to the flow cell priming section, and instructions for topping up the flow cell with fuel, following the release of the Flow Cell Priming Kit (EXP-FLP002)
May 2nd, 2019RBK_9054_v2_revE_23Jan2018English- Download Updates for MinKNOW release 19.05
December 18th, 2018RBK_9054_v2_revD_23Jan2018English- Download Updates for MinKNOW v18.12.4
September 3rd, 2018RBK_9054_v2_revC_23Jan2018English- Download Updates for MinKNOW v2.2
July 19th, 2018RBK_9054_v2_revB_23Jan2018English- Download Updates for MinKNOW GUI v2.0
March 22nd, 2018RBK_9054_v2_revA_23Jan2018English- Download -
January 11th, 2018RBK_9054_v2_revA_02Jan2018English- Download -