Restriction enzyme Pore-C for plant samples
1.Day 1: 1 hour hands-on time2.Cryogrinding: 20 minutes hands-on time3.Nuclei isolation: 1.5 hours hands-on time, 1.5 hours procedure4.Chromatin denaturation: 10 minutes hands-on time, 40 minutes procedure5.Day 2: 5–10 minutes hands-on time, 6-hour procedure time + overnight6.Proximity ligation A with heat denaturation: 5 minutes hands-on time, 6-hour procedure7.Proximity ligation B with heat denaturation: 5 minutes hands-on time, 6-hour procedure8.Protein degradation and de-crosslinking: 5 minutes hands-on time, 5 minutes procedure9.Day 3: DNA extraction: 40 minutes hands-on time, 110 minutes procedure
| Date | Version number | Language | Device | Changes | |
|---|---|---|---|---|---|
| Jun 24 2022 | English |