From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
3rd Dec 2020
Updated the "Notes on multiple crRNAs" in the Preparing the Cas9 ribonucleoprotein complexes (RNPs) step to say:
If you have validated a set of crRNA probes that are always run together, we recommend pooling all crRNA probes and then aliquoting that pool and freezing each aliquot at -80°C. Each time you run a Cas9 experiment and need to make an RNP complex, take out a crRNA pool aliquot and combine with the tracrRNA.
3rd Dec 2020
Updated the "Notes on multiple crRNAs" in the Preparing the Cas9 ribonucleoprotein complexes (RNPs) step to say:
If you have validated a set of crRNA probes that are always run together, we recommend pooling all crRNA probes and then aliquoting that pool and freezing each aliquot at -80°C. Each time you run a Cas9 experiment and need to make an RNP complex, take out a crRNA pool aliquot and combine with the tracrRNA.