There are multiple ways to demultiplex barcoded reads generated by Oxford Nanopore sequencing.

When starting a run in MinKNOW with live basecalling enabled, you can select the barcoding kits you have used and the software will separate your reads into barcoded folders.

In addition, MinKNOW offers a post-run demultiplexing function as described here.

Stand-alone Dorado may also be used from the command line to demultiplex after sequencing. Please refer to the Dorado Documentation for more details.