There are multiple ways to demultiplex barcoded reads generated by Nanopore sequencing.

When starting a run in MinKNOW with live basecalling enabled, you can select the barcoding kits you have used and the software will separate your reads into barcoded folders containing the selected files.

In addition, MinKNOW offers a post-run demultiplexing function as described here.

Stand-alone Dorado may also be used from the command line to demultiplex after sequencing. Please refer to the Dorado GitHub for more details.