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Guppy

Guppy is a data processing toolkit that contains the Oxford Nanopore Technologies' production basecalling algorithms and several bioinformatic post-processing features.

Note:

This software is no longer supported, and we recommend all customers to upgrade to the latest basecaller, Dorado.

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Version: 6.5.7

Release date: 11/15/2023

Approved use: For research use only

Features:

  • Added m6A DRACH-context modified base model for use with RNA004
  • Added poly(A)/poly(T) tail length estimation for direct RNA and cDNA
  • Added RNA read splitting to separate concatenated reads and enabled RNA adapter trimming
  • Reductions to memory usage when performing duplex basecalling
  • Bug fixes, including addressing auto batch size issues and demux crashes

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