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Dorado
High-performance, easy-to-use commandline basecaller for Nanopore data. Developer release for evaluation of new features. For information about the Dorado project, see https://github.com/nanoporetech/dorado.
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Dorado documentation
For more detailed advice, documentation and installation guides for DoradoVersion: 2.1.0
Release date: 7/13/2026
Approved use: For research use only
Performance improvements for v6.0.0 HAC models on Blackwell GPUs
Performance of v5.0.0+ SUP models has also been improved, especially for short reads
Feature improvements and bug fixes for Dorado smallvar:
- Improve the CPU efficiency of read splitting.
- Support gVCF output from Dorado
smallvarvia the--gvcfoption. - Correctly report variants in Dorado
smallvarwhen the reference is lower case. - Sort and deduplicate processed_regions.bed file in Dorado
smallvar. - Add
--hemizygous-regionsoption to Doradosmallvar, to specify regions in which haploid variant calls are expected. - Remove duplicated fileformat header line from VCF output.
- Bug fix for overlapping simple variants by removing redundant allele coverage check in Dorado
smallvar. - Fix output of overlapping variants due to trimming of processed regions before merging.
Other updates and improvements:
- Add
--MD,--csand--rmqto the list of supported minimap options. - No longer create a .fai index file on disk alongside alignment references.
- Fix
sp:itag to contain the correct split points in all cases when a read has been split multiple times. - Add support for single-ended dual barcode arrangements (two barcodes at one end of a read) and arrangements with split inter-barcode flanking regions.
- Update to minimap2 v2.31.
- Pin POD5 version to 0.3.39.
- Fix batch size benchmarking while running LSTM models with variable chunk sizes.
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