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Dorado

High-performance, easy-to-use commandline basecaller for Nanopore data. Developer release for evaluation of new features. For documentation see https://github.com/nanoporetech/dorado

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Dorado documentation
For more detailed advice, documentation and installation guides for Dorado

Version: 1.0.0

Release date: 5/21/2025

Approved use: For research use only

  • New v5.2 basecalling model support for DNA and RNA, including more accurate HAC basecalling and increased canonical basecalling accuracy in DNA with high 6mA density
  • New 2'Ome modification models for RNA: 2OmeG, m5C_2OmeC, inosine_m6A_2OmeA, and pseU_2OmeU
  • More accurate DNA modification models
  • Significant speed enhancements to all-context modification models in both DNA and RNA, when using NVIDIA GPUs
  • A preview release of the new Dorado variant command for small variant calling
  • Poly(A) updates, including the new pa:B:i tag
  • Other fixes and improvements, including updates to logging and improved error messages
  • Removal of support for .fast5 files and basecalling models for DNA R10.4.1 4kHz data, DNA R9.4.1, and RNA002