Direct detection of 8-oxo-dG using nanopore sequencing

Genetic oxidative damage predominantly causes modification of guanine into 8-hydroxyguanine (8-oxo-dG), which has the potential to be a disease biomarker. Currently there are no methods to confidently map this modification. Here the authors trained a deep learning model to detect 8-oxo-dG from raw nanopore sequencing signals. This method allows the simultaneous measure of 5-mC and 8-oxo-dG, providing a new way to explore epigenetics.