High-throughput DNA library preparation with DreamPrep NGS and Ligation Sequencing Kit XL V14 for nanopore sequencing

Overview

DNA sequencing can answer a range of biological questions, from providing information on pathogen identity and genetic disease risk, to helping us to understand evolutionary processes. Oxford Nanopore sequencing generates reads of any length — unlocking more of the genetic code and providing comprehensive genomic insights by accurately capturing complex and repetitive regions in single reads, including methylation patterns.

This application note demonstrates that the Ligation Sequencing Kit V14 (SQK-LSK114) can be automated on the DreamPrep NGS platform to increase consistency and reproducibility for high-throughput DNA sequencing.

In this application note, you will:

• Learn the importance of automated high-output DNA nanopore sequencing

• Discover the methods and analysis tools used to generate high-quality data

• View our benchmarking data generated using nanopore sequencing

Workflow: large cohort sequencing

To learn more about the protocol in this application note, you can download the end-to-end workflow for sequencing large cohorts of clinical research samples using the PromethION 24 sequencing device.