Calling methylation in the human genome with PCR-free nanopore sequencing

Overview

Methylation plays a fundamental role in regulating gene expression; aberrant methylation patterns are strongly associated with numerous diseases, such as cancer and developmental disorders. With PCR-free nanopore sequencing of native DNA, methylation can be directly detected at single-nucleotide resolution. This end-to-end workflow provides a simple solution for genome-wide methylation calling from a human blood research sample.

In this workflow, you will:

• Find out how PCR-free nanopore sequencing enhances methylation detection
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices