PromethION
PromethION offers the same real-time, long-read, direct DNA and RNA sequencing technology as MinION and GridION, at much larger scale. You may find PromethION suitable for large scale projects such as population-scale sequencing or plant genomics, or for large scale service offerings; PromethION is designed to deliver sub-$1,000 human genomes.
The PromethION 24 and PromethION 48 systems offer the ability to run 24 and 48 flow cells respectively. The system allows on-demand sequencing, using any flow cell at any time. Users can start and stop running individual experiments as required, or deploy multiple flow cells onto single experiments for greater speed or throughput.
Latest performance metrics
The performance of nanopore sequencing - whether that is data yields, accuracy or ease of use - has improved rapidly in recent months and years. Each PromethION Flow Cells allows up to 3,000 nanopores to be sequencing simultaneously, and in internal testing a P48 run yielded >7.3 Tb, with further future improvements that will be able to yield much as 15 Tb. You can review what to expect from your PromethION sequencing performance here.
PromethION Beta
With earlier users, no longer available at the store.
* based on a performance of ~158 Gb per flow cell on average. Best internal results are ~220 Gb from a single PromethION Flow Cell
Integrated compute allows real-time basecalling and onward analysis.
Users can choose between capital-free Starter Pack or Capex options, making the PromethION accessible to more laboratories.
Why PromethION?
PromethION system overview
Models
The PromethION hardware consists of 2 versions, the PromethION 24 (P24) can operate up to 24 flow cells at once and the PromethION 48 (P48) can operate up to 48 flow cells at once.
The PromethION system
- Each PromethION flow cell allows up to 3,000 nanopores to be sequencing simultaneously.
- Using current chemistry (early 2019), the best internal yield of a single flow cell is ~220 Gb and the best external result 148 Gb. Yield will depend on the application, extraction and preparation methods.
- PromethION uses the same nanopore sequencing technology as MinION/GridION:
- PromethION flow cells are compatible with PromethION 24 and PromethION 48 (and also PromethION Beta).
- Flow Cells can be used with gDNA, RNA and cDNA Library preparation kits.
- Sample input amount is the same as MinION/ GridION.
- Analysis and informatics same as other Nanopore data.
- At 158 Gb per flow cell, P24 has the capacity to deliver 3.8 Tb in 72 hours with further improvements in the pipeline
- Suitable for groups with mid to large size projects who wish to have high throughput, long read, direct DNA or RNA sequence data
- Compute module: Contains latest gen processors and GPU technology for real time analysis
- 384 Gb RAM
- 32 TB SSD
- At 158 Gb per flow cell, P48 has the capacity to deliver 7.6 Tb in 72 hours with further improvements in the pipeline
- Suitable for groups with larger-scale projects that require a full capacity device for very high throughput, long read, direct DNA or RNA sequence data
- Compute module: Contains latest gen processors and GPU technology for real time analysis
- 384 Gb RAM
- 32 TB SSD
- Users can choose between capital-free Starter Packs or Capital purchase options, making the PromethION accessible to more laboratories.
- All purchasing models include the provision of flow cells and kits enabling users to get started straight away.
Users with Beta devices wishing to understand their upgrade options should contact support
