RNA library preparation
Revolutionise your RNA research
The RNA library preparation kits offer a range of benefits, including high accuracy, long reads, and fast turnaround times. Every research project is unique, so to cater to different research projects, a range of kits are available. Confidently characterise and quantify full-length RNA transcripts, splice variants, and fusions using short to ultra-long fragment Oxford Nanopore sequencing. Accurately analyse differential gene expression and transcript usage. Sequence native RNA directly, without amplification or reverse transcription, and identify base modifications.
PCR-free protocols to elimate bias
Library for full-length transcripts sequencing
cDNA sequencing
Base modifications preserved
| Direct RNA | cDNA | |
|---|---|---|
| Direct RNA Sequencing Kit | cDNA-PCR Sequencing Kit | |
| Preparation time | 135 min | ~225 mins + PCR |
| Input recommendation | 300 ng poly(A)+ RNA or 1 μg total RNA | 10 ng poly(A)+ RNA or 500 ng total RNA |
| Reverse transcription | Optional | Yes |
| Amplification | No | Yes |
| Barcode options | In development | cDNA-PCR Barcoding Kit (24 plex) |
| Typical output | 20M reads | >100M reads |
| Methylation included | Yes | - |
