Resources: Documentation

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10X Genomics GEM-X Single Cell 3' Kit (V4), Next-GEM Single Cell 3' Kit (V3.1), or the Visium Spatial Gene Expression Kit (V1) - Library preparation time ~205 minutes - High output - PCR required For Research Use Only

RT-PCR for detecting the SARS-CoV-2 virus

For Research Use Only

This protocol: - is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. - consists of a two-in-one method that splits sample into bacterial/fungal sample preparation and viral/acellular sample preparation. - provides a recommended host depletion and extraction method for respiratory samples. - uses a shotgun approach to RT-PCR. - involves tagmentation, barcoding and PCR amplification. - is compatible with R10.4.1 flow cells. <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

The fastest and simplest protocol for full-length cDNA sequencing - Offering highest yield - Higher yields than traditional cDNA synthesis - Splice variants and fusion transcripts - Compatible with R10.4.1 flow cells <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

The fastest and simplest protocol for full-length cDNA sequencing - Offering highest yield - Higher yields than traditional cDNA synthesis - Splice variants and fusion transcripts - Multiplex up to 24 different samples - Compatible with R10.4.1 flow cells only <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10X Genomics GEM-X Single Cell 5' Kit (V3) or the Next GEM Single Cell 5' Kit (V2) - Library preparation time ~135 minutes - High output - PCR required For Research Use Only

The PCR-free protocol for full-length cDNA offers: - Higher yields than traditional cDNA synthesis - Analysis of splice variants & fusion transcripts - Compatibility with R10.4.1 flow cells For Research Use Only

For Research Use Only

This protocol: - Is for sequencing native RNA - Can be used with total RNA or an enriched sample (e.g. poly(A) tailed or ribo-depleted) as a starting input material - Requires no fragmentation - Takes approximately 135 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__

This protocol: - Is for sequencing native RNA - Can be used with total RNA or an enriched sample (e.g. poly(A) tailed or ribo-depleted) as a starting input material - Requires no fragmentation - Takes approximately 135 minutes for library preparation. - Is only compatible with RNA flow cells __This is an Early Access product.__ __For Research Use Only__

This protocol: - is for selecting RNA targets of your choice for sequencing native RNA. - uses total RNA as starting input material. - requires no fragmentation. - takes ~2 hours for library preparation. For Research Use Only __This is an Early Access product.__ __For more information about our Early Access programmes, please see this [article on product release phases.](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you)__

This protocol: - Is for a control experiment using kit-supplied RNA - Sequences native RNA - Requires no fragmentation - Takes approximately 135 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__