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Telomere-to-telomere sequencing (T2T) from blood and cells using SQK-APK114, SQK-LSK114, and SQK-ULK114 (T2T_9211_v114_revG_09May2025)

DateVersionLanguageDeviceChanges
5/9/2025T2T_9211_v114_revG_09May2025EnglishPromethION
09 May 2025 - Protocol title update
2/25/2025T2T_9211_v114_revF_27Nov2024EnglishPromethION
19th February 2025 - Added link to the Telomere-to-telomere sequencing (T2T) know-how document in the protocol introduction.
11/26/2024T2T_9211_v114_revF_27Nov2024EnglishPromethION
27th November 2024 - SQK-ULK114 kit content updated with new kit layout and Precipitation Star (PS) information. - In the Ultra-long sequencing experiment: added optional step for use of Precipitation Star (PS) in clean-up following DNA tagmentation.
11/1/2024T2T_9211_v114_revE_04Sep2024EnglishPromethION
1st November 2024 - Updated "Overview of the protocol": Removal of reference to Herro within the "Sequencing and analysis" section in reference to "Assembly Polishing Kit experiment". Now references use of Dorado for read correction. - Included instructions for obtaining and installing the SQK-APK114 sequencing script on your device in the "Data acquisition and basecalling: Assembly Polishing Kit" section of the protocol. - Updated formatting for the "Data acquisition and basecalling" pages of the protocol to more easily separate out the parameters of each section.
11/1/2024T2T_9211_v114_revD_04Sep2024EnglishPromethION
1st November 2024 - Updated "Overview of the protocol": Removal of reference to Herro within the "Sequencing and analysis" section in reference to "Assembly Polishing Kit experiment". Now references use of Dorado for read correction. - Included instructions for obtaining and installing the SQK-APK114 sequencing script on your device in the "Data acquisition and basecalling: Assembly Polishing Kit" section of the protocol. - Updated formatting for the "Data acquisition and basecalling" pages of the protocol to more easily separate out the parameters of each section.
9/24/2024T2T_9211_v114_revC_04Sep2024EnglishPromethION
24th September 2024 - Medaka model pinned to version 1.12.1 to avoid discrepancy issues in downstream analysis.
9/10/2024T2T_9211_v114_revB_04Sep2024EnglishPromethION
10th September 2024 - Updated setting recommendations in downstream analysis.
9/9/2024T2T_9211_v114_revA_04Sep2024EnglishPromethION
09th September 2024 Protocol release
9/6/2024Version2a_Rev14EnglishPromethION
04th September 2024 Protocol release
8/30/2024Version2a_Rev13EnglishPromethION
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