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PromethION 24 A100

PromethION devices deliver flexible, high-capacity, benchtop sequencing ideal for large-scale projects and high-throughput laboratories. PromethION 24 A100 (P24 A100) is designed to run up to 24 flow cells — delivering yields of up to 7 Tbases. Each flow cell can be run independently, providing on-demand, long-read sequencing to suit all experimental requirements. Integrated, high-performance compute allows real-time base calling and onward analysis for rapid access to results.

PromethION 24 A100 is a high-throughput nanopore sequencing device using the same technology found in the MinION and GridION devices, but massively paralleled. It allows up to 24 sequencing experiments to be run concurrently or individually. PromethION also allows users to offer nanopore sequencing as a service.

The PromethION 24 A100 consists of a Sequencing Unit with 24 sequencing ports where PromethION flow cells can be inserted, as well as an A100 Data Acquisition Unit offering a high performance integrated computer and basecalling accelerators. The device can basecall, in real-time, the data generated by 24 flow cells. The current chemistry and software enables generation of up to 200 Gbases of data from a single PromethION flow cell.

Setting up a PromethION 24 A100 requires minimal to no facility upgrades. A new device requires a power source, network connectivity via copper or fibre, and will utilise existing storage infrastructure. There are USB ports available for peripherals, e.g. keyboard and mouse. Monitors must be connected via HDMI.

The Oxford Nanopore Technologies PromethION 24 A100 device is stored and shipped at ambient temperature (15–25°C).

Please note that the PromethION 24 A100 is shipped separately to the kits and flow cells.

PromethION IT requirements

Oxford Nanopore Technologies® PromethION 24 A100 device is an electronic analysis system for use in scientific research. The core technology is built around a nanopore that is able to detect single molecule events including nucleic acids (DNA/RNA), proteins and small molecules.

This product is for research use only

The safety information below provides you with the details needed to install and use the system safely.

In case of emergency, switch the PromethION 24 A100 off at the power switch and unplug the power cables from the back of the device.

The software licence and device warranty contract ensures your instrument is performing optimally by providing the latest up-to-date hardware and software. The contract guarantees that Oxford Nanopore Technologies support obligations are delivered during the contract period as laid out in sections 4 and 7 of the Nanopore Product Terms and Conditions.

This includes:

• Software updates upon release
• Hardware updates on release
• Return and Replace policy

The service contract extends our warranty to cover the instrument after your initial purchase contract has expired.

The PromethION 24 A100 is shipped together with the necessary cables and Configuration Test Cells to confirm your hardware is functioning as expected.

Configuration is the process of testing that communication between the PromethION device and the control software is operational prior to experimental work being performed. This is carried out in the absence of any chemistry and uses a specific flow cell known as the Configuration Test Cell (CTC).

The PromethION 24 A100 is packed into two boxes that contain everything needed for installing the device. The shipping weight is ~30 kg, meaning no special equipment is required for installing the PromethION in your laboratory.

The PromethION 24 A100 can be used together with:

Flow cells

• FLO-PRO002
• FLO-PRO114M

Kits FLO-PRO002 flow cells are suitable for:

• Ligation Sequencing Kit (SQK-LSK110)
• Ligation Sequencing Kit (SQK-LSK109)
• PCR-cDNA Sequencing Kit (SQK-PCS111)
• PCR-cDNA Sequencing Kit (SQK-PCS109)
• PCR-cDNA Barcoding Kit (SQK-PCB109)
• Direct cDNA Sequencing Kit (SQK-DCS109)
• Direct RNA Sequencing Kit (SQK-RNA002)

FLO-PRO114M flow cells are suitable for V14 Sequencing kit:

• Ligation Sequencing Kit V14 (SQK-LSK114)
• Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
• Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Native Barcoding Kit 96 V14 (SQK-NBD114.96)
• Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)
• Rapid Barcoding Kit 24 V14 (SQK-RBK114.24)
• Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)

Software Basecalling:

• MinKNOW
• Guppy

Downstream analysis:

• EPI2ME
• Oxford Nanopore-developed tools and pipelines
• Customer-developed tools and pipelines