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Amplicon sequencing from DNA using SQK-RBK114 (.24 or .96) (RAA_9198_v114_revL_09May2025)

DateVersionLanguageDeviceChanges
12/11/2024RAA_9198_v114_revK_11Dec2024EnglishGridION
11 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024RAA_9198_v114_revK_11Dec2024EnglishMinION
11 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024RAA_9198_v114_revJ_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/15/2024RAA_9198_v114_revJ_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
9/30/2024RAA_9198_v114_revI_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RAA_9198_v114_revI_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
4/29/2024RAA_9198_v114_revH_29Nov2023EnglishGridION
29 April 2024 - Updated references to EPI2ME (no longer called EPI2ME Labs) throughout document.
4/29/2024RAA_9198_v114_revH_29Nov2023EnglishMinION
29 April 2024 - Updated references to EPI2ME (no longer called EPI2ME Labs) throughout document.
November 30th, 2023RAA_9198_v114_revA_29Nov2023EnglishMinion Download -
November 30th, 2023RAA_9198_v114_revA_29Nov2023EnglishGridion Download -
April 29th, 2024RAA_9198_v114_revG_29Nov2023EnglishGridion Download 02 April 2024 - Added note to 'Introduction to amplicon sequencing using the Rapid Barcoding Kit 24 or 96 V14' to specify correct primer design prior to amplicon generation. - Added "Important" section to 'Equipment and Consumables' highlighting primer design and consideractions prior to amplcion generation.
April 29th, 2024RAA_9198_v114_revG_29Nov2023EnglishMinion Download 02 April 2024 - Added note to 'Introduction to amplicon sequencing using the Rapid Barcoding Kit 24 or 96 V14' to specify correct primer design prior to amplicon generation. - Added "Important" section to 'Equipment and Consumables' highlighting primer design and consideractions prior to amplcion generation.
April 2nd, 2024RAA_9198_v114_revF_29Nov2023EnglishGridion Download 05 March 2024 - Included amplicon size recommendations in materials.
April 2nd, 2024RAA_9198_v114_revF_29Nov2023EnglishMinion Download 05 March 2024 - Included amplicon size recommendations in materials.
March 5th, 2024RAA_9198_v114_revE_29Nov2023EnglishGridion Download 28 Feb 2024 - Updated wording in introduction to clarify suitability with mixed species amplicon samples.
March 5th, 2024RAA_9198_v114_revE_29Nov2023EnglishMinion Download 28 Feb 2024 - Updated wording in introduction to clarify suitability with mixed species amplicon samples.
February 28th, 2024RAA_9198_v114_revD_29Nov2023EnglishGridion Download January 2024 - Added wording throughout protocol to clarify each sample/barcode should be associated to a single species amplicon (this method is not suitable for mixed species amplicon samples).
February 28th, 2024RAA_9198_v114_revD_29Nov2023EnglishMinion Download January 2024 - Added wording throughout protocol to clarify each sample/barcode should be associated to a single species amplicon (this method is not suitable for mixed species amplicon samples).
January 2nd, 2024RAA_9198_v114_revC_29Nov2023EnglishGridion Download December 2023 - Updated wf-amplicon set-up image for Downsampling
January 2nd, 2024RAA_9198_v114_revC_29Nov2023EnglishMinion Download December 2023 - Updated wf-amplicon set-up image for Downsampling
December 18th, 2023RAA_9198_v114_revB_29Nov2023EnglishGridion Download December 2023 - Updated workflow report links
December 18th, 2023RAA_9198_v114_revB_29Nov2023EnglishMinion Download December 2023 - Updated workflow report links

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