From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
GridION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
MinION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
PromethION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
Flongle
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
GridION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
MinION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
PromethION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
Flongle
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
GridION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
MinION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
English
PromethION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
Flongle
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
GridION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
MinION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RBK_9176_v114_revQ_27Dec2024
中文
PromethION
28th January 2025
- In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
12/27/2024
RBK_9176_v114_revP_27Dec2024
English
Flongle
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
English
GridION
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
English
MinION
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
English
PromethION
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
中文
Flongle
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
中文
GridION
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
中文
MinION
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024
RBK_9176_v114_revP_27Dec2024
中文
PromethION
27th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
11/14/2024
RBK_9176_v114_revO_20Nov2024
English
Flongle
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
English
PromethION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
中文
Flongle
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
中文
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
中文
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024
RBK_9176_v114_revO_20Nov2024
中文
PromethION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
9/30/2024
RBK_9176_v114_revN_30Sept2024
English
Flongle
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
English
PromethION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
中文
Flongle
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
中文
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
中文
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_30Sept2024
中文
PromethION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
English
Flongle
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
English
PromethION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
中文
Flongle
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
中文
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
中文
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RBK_9176_v114_revN_27Nov2022
中文
PromethION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
4/3/2024
RBK_9176_v114_revM_27Nov2022
English
Flongle
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
English
GridION
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
English
MinION
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
English
PromethION
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
中文
Flongle
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
中文
GridION
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
中文
MinION
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024
RBK_9176_v114_revM_27Nov2022
中文
PromethION
January 2024
- Protocol format up-rev to new format
- Input quantities updated to 200 ng per sample
- Included a minimum required use of 4 barcodes
- Updated Rapid barcode addition volume
- Updated pooling and clean-up instructions
- Elute volumes updated to 15ul of EB per 24 samples
- PromethION branch library formulation updated to use EB (was nuclease-free water)
January 2023
- Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 2023
- Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 2023
- Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 2023
- Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
UNPUBLISHED
January 2023
- Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
UNPUBLISHED
January 2023
- Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
UNPUBLISHED
January 2023
- Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
UNPUBLISHED
January 2023
- Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
- Changed the "Priming and loading the PrometION flow cell" section to the updated version.
November 2022
- Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
- Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 2022
- Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
- Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 2022
- Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
- Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 2022
- Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
- Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul