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Rapid sequencing DNA V14 - barcoding (SQK-RBK114.24 or SQK-RBK114.96) (RBK_9176_v114_revS_27Oct2025)

DateVersionLanguageDeviceChanges
10/27/2025RBK_9176_v114_revS_27Oct2025EnglishFlongle
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025EnglishGridION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025EnglishMinION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025EnglishPromethION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025EspañolMinION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025EspañolPromethION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025中文MinION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
10/27/2025RBK_9176_v114_revS_27Oct2025中文PromethION
27th October 2025 - In 'Overview of the protocol', 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table. - In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited. - In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - For the PromethION Flow Cell loading, the note to take the flow cell out of the fridge was moved to the top of this section. - The 'Data acquisition and basecalling' section was edited and formatted to align with current protocols.
9/18/2025RBK_9176_v114_revR_04Jun2025EnglishFlongle
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025EnglishGridION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025EnglishMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025EnglishPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025EspañolMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025EspañolPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025中文MinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
9/18/2025RBK_9176_v114_revR_04Jun2025中文PromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025EnglishFlongle
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025EnglishGridION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025EnglishMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025EnglishPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025EspañolMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025EspañolPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025中文MinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
8/21/2025RBK_9176_v114_revR_04Jun2025中文PromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025EnglishFlongle
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025EnglishGridION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025EnglishMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025EnglishPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025EspañolMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025EspañolPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025中文MinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
7/18/2025RBK_9176_v114_revR_04Jun2025中文PromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025EnglishFlongle
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025EnglishGridION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025EnglishMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025EnglishPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025EspañolMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025EspañolPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025中文MinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/5/2025RBK_9176_v114_revR_04Jun2025中文PromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025EnglishFlongle
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025EnglishGridION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025EnglishMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025EnglishPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025EspañolMinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025EspañolPromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025中文MinION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
6/4/2025RBK_9176_v114_revR_04Jun2025中文PromethION
04th June 2025 - Updated elution instructions in the library preparation section of the protocol to clarify minimum elution volume as 15 ul.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishFlongle
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishGridION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishMinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishPromethION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
3/26/2025RBK_9176_v114_revQ_27Dec2024EspañolMinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文Flongle
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文GridION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文MinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文PromethION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishFlongle
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishGridION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishMinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024EnglishPromethION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文Flongle
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文GridION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文MinION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025RBK_9176_v114_revQ_27Dec2024中文PromethION
28th January 2025 - In the Flongle branch of "Equipment and consumables", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
12/27/2024RBK_9176_v114_revP_27Dec2024EnglishFlongle
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024EnglishGridION
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024EnglishMinION
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024EnglishPromethION
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024中文Flongle
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024中文GridION
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024中文MinION
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024RBK_9176_v114_revP_27Dec2024中文PromethION
27th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
11/14/2024RBK_9176_v114_revO_20Nov2024EnglishFlongle
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024EnglishPromethION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024中文Flongle
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024中文GridION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024中文MinION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/14/2024RBK_9176_v114_revO_20Nov2024中文PromethION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
9/30/2024RBK_9176_v114_revN_30Sept2024EnglishFlongle
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024EnglishPromethION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024中文Flongle
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024中文GridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024中文MinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_30Sept2024中文PromethION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022EnglishFlongle
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022EnglishPromethION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022中文Flongle
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022中文GridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022中文MinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024RBK_9176_v114_revN_27Nov2022中文PromethION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
4/3/2024RBK_9176_v114_revM_27Nov2022EnglishFlongle
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022EnglishGridION
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022EnglishMinION
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022EnglishPromethION
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022中文Flongle
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022中文GridION
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022中文MinION
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
4/3/2024RBK_9176_v114_revM_27Nov2022中文PromethION
January 2024 - Protocol format up-rev to new format - Input quantities updated to 200 ng per sample - Included a minimum required use of 4 barcodes - Updated Rapid barcode addition volume - Updated pooling and clean-up instructions - Elute volumes updated to 15ul of EB per 24 samples - PromethION branch library formulation updated to use EB (was nuclease-free water)
January 24th, 2024RBK_9176_v114_revL_27Nov2022EnglishMinion Download 3rd October 2023 - Updated checklist formatting
January 24th, 2024RBK_9176_v114_revL_27Nov2022EnglishPromethion Download 3rd October 2023 - Updated checklist formatting
January 24th, 2024RBK_9176_v114_revL_27Nov2022EnglishFlongle Download 3rd October 2023 - Updated checklist formatting
January 24th, 2024RBK_9176_v114_revL_27Nov2022EnglishGridion Download 3rd October 2023 - Updated checklist formatting
October 3rd, 2023RBK_9176_v114_revK_27Nov2022EnglishPromethion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
October 3rd, 2023RBK_9176_v114_revK_27Nov2022EnglishFlongle Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
October 3rd, 2023RBK_9176_v114_revK_27Nov2022EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
October 3rd, 2023RBK_9176_v114_revK_27Nov2022EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023RBK_9176_v114_revJ_27Nov2022EnglishPromethion Download 24th April 2023 - Updated the volume of DNA sample going into the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
September 19th, 2023RBK_9176_v114_revJ_27Nov2022EnglishFlongle Download 24th April 2023 - Updated the volume of DNA sample going into the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
September 19th, 2023RBK_9176_v114_revJ_27Nov2022EnglishGridion Download 24th April 2023 - Updated the volume of DNA sample going into the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
September 19th, 2023RBK_9176_v114_revJ_27Nov2022EnglishMinion Download 24th April 2023 - Updated the volume of DNA sample going into the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
March 3rd, 2023RBK_9176_v114_revI_27Nov2022EnglishPromethion Download March 2023 - Updated the volume for the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
March 3rd, 2023RBK_9176_v114_revI_27Nov2022EnglishFlongle Download March 2023 - Updated the volume for the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
March 3rd, 2023RBK_9176_v114_revI_27Nov2022EnglishGridion Download March 2023 - Updated the volume for the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
March 3rd, 2023RBK_9176_v114_revI_27Nov2022EnglishMinion Download March 2023 - Updated the volume for the addition of Rapid Adapter in the Flongle branch for correct adapter-sample ratio.
March 1st, 2023RBK_9176_v114_revH_27Nov2022EnglishPromethion Download March 2023 - Updated the "Library preparation" step in the PromethION branch to remove the extra step to dilute in EB.
March 1st, 2023RBK_9176_v114_revH_27Nov2022EnglishFlongle Download March 2023 - Updated the "Library preparation" step in the PromethION branch to remove the extra step to dilute in EB.
March 1st, 2023RBK_9176_v114_revH_27Nov2022EnglishGridion Download March 2023 - Updated the "Library preparation" step in the PromethION branch to remove the extra step to dilute in EB.
March 1st, 2023RBK_9176_v114_revH_27Nov2022EnglishMinion Download March 2023 - Updated the "Library preparation" step in the PromethION branch to remove the extra step to dilute in EB.
January 16th, 2023RBK_9176_v114_revG_27Nov2022EnglishPromethion Download January 2023 - Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 16th, 2023RBK_9176_v114_revG_27Nov2022EnglishFlongle Download January 2023 - Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 16th, 2023RBK_9176_v114_revG_27Nov2022EnglishGridion Download January 2023 - Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 16th, 2023RBK_9176_v114_revG_27Nov2022EnglishMinion Download January 2023 - Updated "Priming and loading the flow cell" pages to clarify distinction in preparation of the flow cell priming mix depending on use of RBK114.24 kit or RBK114.96 kit.
January 11th, 2023RBK_9176_v114_revF_27Nov2022EnglishPromethion Download January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
January 11th, 2023RBK_9176_v114_revF_27Nov2022EnglishFlongle Download January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
January 11th, 2023RBK_9176_v114_revF_27Nov2022EnglishGridion Download January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
January 11th, 2023RBK_9176_v114_revF_27Nov2022EnglishMinion Download January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables.
January 6th, 2023RBK_9176_v114_revE_27Nov2022EnglishPromethion Download UNPUBLISHED January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables. - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023RBK_9176_v114_revE_27Nov2022EnglishFlongle Download UNPUBLISHED January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables. - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023RBK_9176_v114_revE_27Nov2022EnglishGridion Download UNPUBLISHED January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables. - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023RBK_9176_v114_revE_27Nov2022EnglishMinion Download UNPUBLISHED January 2023 - Included kit contents information for the Flongle Sequencing Expansion (EXP-FSE002) in the equipment and consumables. - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
December 15th, 2022RBK_9176_v114_revD_27Nov2022EnglishPromethion Download December 2022 - Added information regarding the Rapid Adapter Auxiliary V14 expansion in the Equipment and Consumables section
December 15th, 2022RBK_9176_v114_revD_27Nov2022EnglishFlongle Download December 2022 - Added information regarding the Rapid Adapter Auxiliary V14 expansion in the Equipment and Consumables section
December 15th, 2022RBK_9176_v114_revD_27Nov2022EnglishGridion Download December 2022 - Added information regarding the Rapid Adapter Auxiliary V14 expansion in the Equipment and Consumables section
December 15th, 2022RBK_9176_v114_revD_27Nov2022EnglishMinion Download December 2022 - Added information regarding the Rapid Adapter Auxiliary V14 expansion in the Equipment and Consumables section
November 30th, 2022RBK_9176_v114_revC_27Nov2022EnglishPromethion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002) - Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 30th, 2022RBK_9176_v114_revC_27Nov2022EnglishFlongle Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002) - Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 30th, 2022RBK_9176_v114_revC_27Nov2022EnglishGridion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002) - Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 30th, 2022RBK_9176_v114_revC_27Nov2022EnglishMinion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002) - Updated "Library preparation" for Flongle flow cells: Finla library volume and loading input updated to 200 ng in 5 ul
November 27th, 2022RBK_9176_v114_revA_27Nov2022EnglishPromethion Download -
November 27th, 2022RBK_9176_v114_revA_27Nov2022EnglishFlongle Download -
November 27th, 2022RBK_9176_v114_revA_27Nov2022EnglishGridion Download -
November 27th, 2022RBK_9176_v114_revA_27Nov2022EnglishMinion Download -

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