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cDNA-PCR Sequencing V14 - Barcoding (SQK-PCB114.24) (PCB_9201_v114_revG_10Dec2025)

DateVersionLanguageDeviceChanges
12/10/2025PCB_9201_v114_revG_10Dec2025EnglishGridION
10th December 2025 - In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy. - In sections 3 and 6 for the device branches, the link for the flow cell check was updated. - In section 4, an important note was included stating that if using the barcode primers in plate format, to only use the wells once. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW. - In 'Troubleshooting', section 11, Guppy was removed as it is no longer applicable.
12/10/2025PCB_9201_v114_revG_10Dec2025EnglishMinION
10th December 2025 - In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy. - In sections 3 and 6 for the device branches, the link for the flow cell check was updated. - In section 4, an important note was included stating that if using the barcode primers in plate format, to only use the wells once. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW. - In 'Troubleshooting', section 11, Guppy was removed as it is no longer applicable.
12/10/2025PCB_9201_v114_revG_10Dec2025EnglishPromethION
10th December 2025 - In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy. - In sections 3 and 6 for the device branches, the link for the flow cell check was updated. - In section 4, an important note was included stating that if using the barcode primers in plate format, to only use the wells once. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW. - In 'Troubleshooting', section 11, Guppy was removed as it is no longer applicable.
12/10/2025PCB_9201_v114_revG_10Dec2025中文GridION
10th December 2025 - In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy. - In sections 3 and 6 for the device branches, the link for the flow cell check was updated. - In section 4, an important note was included stating that if using the barcode primers in plate format, to only use the wells once. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW. - In 'Troubleshooting', section 11, Guppy was removed as it is no longer applicable.
12/10/2025PCB_9201_v114_revG_10Dec2025中文MinION
10th December 2025 - In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy. - In sections 3 and 6 for the device branches, the link for the flow cell check was updated. - In section 4, an important note was included stating that if using the barcode primers in plate format, to only use the wells once. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW. - In 'Troubleshooting', section 11, Guppy was removed as it is no longer applicable.
12/10/2025PCB_9201_v114_revG_10Dec2025中文PromethION
10th December 2025 - In section 1, 'Overview of the protocol', in the Table under 'Library preparation', timing for the flow cell priming and loading was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and MinION Mk1C along with their links to the IT requirements documents were removed as these documents have moved to legacy. - In sections 3 and 6 for the device branches, the link for the flow cell check was updated. - In section 4, an important note was included stating that if using the barcode primers in plate format, to only use the wells once. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 7, 'Data acquisition and basecalling', links for the MinION Mk1B and MinION Mk1C user manuals were removed as these documents have moved to legacy. And in 'To start a sequencing run in MinKNOW', point 3, the kit used for sequencing was specified for the kit selection page on MinKNOW. - In 'Troubleshooting', section 11, Guppy was removed as it is no longer applicable.
11/25/2025PCB_9201_v114_revF_02Jul2025EnglishGridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
11/25/2025PCB_9201_v114_revF_02Jul2025EnglishMinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
11/25/2025PCB_9201_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
11/25/2025PCB_9201_v114_revF_02Jul2025中文GridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
11/25/2025PCB_9201_v114_revF_02Jul2025中文MinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
11/25/2025PCB_9201_v114_revF_02Jul2025中文PromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
10/17/2025PCB_9201_v114_revF_02Jul2025EnglishGridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
10/17/2025PCB_9201_v114_revF_02Jul2025EnglishMinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
10/17/2025PCB_9201_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
10/17/2025PCB_9201_v114_revF_02Jul2025中文GridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
10/17/2025PCB_9201_v114_revF_02Jul2025中文MinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
10/17/2025PCB_9201_v114_revF_02Jul2025中文PromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025EnglishGridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025EnglishMinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025中文GridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025中文MinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025中文PromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025PCB_9201_v114_revF_02Jul2025EnglishGridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025PCB_9201_v114_revF_02Jul2025EnglishMinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025PCB_9201_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/22/2025PCB_9201_v114_revF_02Jul2025中文GridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/22/2025PCB_9201_v114_revF_02Jul2025中文MinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/22/2025PCB_9201_v114_revF_02Jul2025中文PromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
6/6/2025PCB_9201_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
6/6/2025PCB_9201_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
6/6/2025PCB_9201_v114_revE_11Dec2024EnglishPromethION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024PCB_9201_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024PCB_9201_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024PCB_9201_v114_revE_11Dec2024EnglishPromethION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
4/19/2024PCB_9201_v114_revD_06Dec2023EnglishGridION
19 April 2024 - Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage. - Removed storage recommendations following Rapid Adapter addition
4/19/2024PCB_9201_v114_revD_06Dec2023EnglishMinION
19 April 2024 - Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage. - Removed storage recommendations following Rapid Adapter addition
4/19/2024PCB_9201_v114_revD_06Dec2023EnglishPromethION
19 April 2024 - Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage. - Removed storage recommendations following Rapid Adapter addition
January 29th, 2024PCB_9201_v114_revB_06Dec2023EnglishGridion Download 21st December 2023 - Updated kit image
January 29th, 2024PCB_9201_v114_revB_06Dec2023EnglishMinion Download 21st December 2023 - Updated kit image
January 29th, 2024PCB_9201_v114_revB_06Dec2023EnglishPromethion Download 21st December 2023 - Updated kit image
December 21st, 2023PCB_9201_v114_revA_06Dec2023EnglishMinion Download -
December 21st, 2023PCB_9201_v114_revA_06Dec2023EnglishGridion Download -
December 21st, 2023PCB_9201_v114_revA_06Dec2023EnglishPromethion Download -

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