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cDNA-PCR Sequencing V14 - Barcoding (SQK-PCB114.24) (PCB_9201_v114_revF_02Jul2025)

DateVersionLanguageDeviceChanges
8/21/2025PCB_9201_v114_revF_02Jul2025EnglishGridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025EnglishMinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025中文GridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025中文MinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
8/21/2025PCB_9201_v114_revF_02Jul2025中文PromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025PCB_9201_v114_revF_02Jul2025EnglishGridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025PCB_9201_v114_revF_02Jul2025EnglishMinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/2/2025PCB_9201_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/22/2025PCB_9201_v114_revF_02Jul2025中文GridION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/22/2025PCB_9201_v114_revF_02Jul2025中文MinION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
7/22/2025PCB_9201_v114_revF_02Jul2025中文PromethION
02 July 2025 - Updated workflow image - Added information for SQK-PCB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Barcode Primers at 10 μM, new plate format with Barcode Primers at 1 μM
6/6/2025PCB_9201_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
6/6/2025PCB_9201_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
6/6/2025PCB_9201_v114_revE_11Dec2024EnglishPromethION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024PCB_9201_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024PCB_9201_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
12/11/2024PCB_9201_v114_revE_11Dec2024EnglishPromethION
11th December 2024 - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated optional instructions for control experiment using EXP-RCS001 and the RNA Control Strand (RCS) to include options for higher and lower concentration of the RCS vial depending on kit batch number.
4/19/2024PCB_9201_v114_revD_06Dec2023EnglishGridION
19 April 2024 - Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage. - Removed storage recommendations following Rapid Adapter addition
4/19/2024PCB_9201_v114_revD_06Dec2023EnglishMinION
19 April 2024 - Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage. - Removed storage recommendations following Rapid Adapter addition
4/19/2024PCB_9201_v114_revD_06Dec2023EnglishPromethION
19 April 2024 - Updated Library storage condition following "Selecting for full-length transcripts by PCR" to 4C for short-term storage. - Removed storage recommendations following Rapid Adapter addition
January 29th, 2024PCB_9201_v114_revB_06Dec2023EnglishGridion Download 21st December 2023 - Updated kit image
January 29th, 2024PCB_9201_v114_revB_06Dec2023EnglishMinion Download 21st December 2023 - Updated kit image
January 29th, 2024PCB_9201_v114_revB_06Dec2023EnglishPromethion Download 21st December 2023 - Updated kit image
December 21st, 2023PCB_9201_v114_revA_06Dec2023EnglishMinion Download -
December 21st, 2023PCB_9201_v114_revA_06Dec2023EnglishGridion Download -
December 21st, 2023PCB_9201_v114_revA_06Dec2023EnglishPromethion Download -

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