13 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024
ISO_9205_v114_revF_13Dec2024
English
MinION
13 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024
ISO_9205_v114_revE_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
11/15/2024
ISO_9205_v114_revE_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
10/14/2024
ISO_9205_v114_revD_14Oct2024
English
GridION
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
10/14/2024
ISO_9205_v114_revD_14Oct2024
English
MinION
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
9/30/2024
ISO_9205_v114_revC_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
ISO_9205_v114_revC_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
5/14/2024
ISO_9205_v114_revB_20Mar2024
English
GridION
27th March 2024
- Updated wording for MinKNOW updates to specify v24.02 or newer
5/14/2024
ISO_9205_v114_revB_20Mar2024
English
MinION
27th March 2024
- Updated wording for MinKNOW updates to specify v24.02 or newer