Main menu

Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96) (ISO_9205_v114_revG_09May2025)


DateVersionLanguageDeviceChanges
6/23/2025ISO_9205_v114_revG_09May2025EnglishGridION
09 May 2025 - Protocol title update
6/23/2025ISO_9205_v114_revG_09May2025EnglishMinION
09 May 2025 - Protocol title update
12/13/2024ISO_9205_v114_revF_13Dec2024EnglishGridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024ISO_9205_v114_revF_13Dec2024EnglishMinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024ISO_9205_v114_revE_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes. 14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
11/15/2024ISO_9205_v114_revE_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes. 14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
10/14/2024ISO_9205_v114_revD_14Oct2024EnglishGridION
14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
10/14/2024ISO_9205_v114_revD_14Oct2024EnglishMinION
14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
9/30/2024ISO_9205_v114_revC_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024ISO_9205_v114_revC_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
5/14/2024ISO_9205_v114_revB_20Mar2024EnglishGridION
27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
5/14/2024ISO_9205_v114_revB_20Mar2024EnglishMinION
27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
May 14th, 2024ISO_v9205_v114_revB_20Mar2024EnglishGridion Download 27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
May 14th, 2024ISO_v9205_v114_revB_20Mar2024EnglishMinion Download 27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
March 20th, 2024ISO_v9205_v114_revA_20Mar2024EnglishGridion Download 20th March 2024 Protocol released
March 20th, 2024ISO_v9205_v114_revA_20Mar2024EnglishMinion Download 20th March 2024 Protocol released

Getting started

Buy a MinION starter pack Nanopore store Sequencing service providers Channel partners

Nanopore technology

Subscribe to Nanopore updates Resources and publications What is the Nanopore Community

About Oxford Nanopore

News Company timeline Sustainability Leadership team Media resources & contacts For investors For partners Working at Oxford Nanopore Current vacancies Commercial information BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
English flag