13 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024
ISO_9205_v114_revF_13Dec2024
English
MinION
13 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024
ISO_9205_v114_revE_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
11/15/2024
ISO_9205_v114_revE_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
10/14/2024
ISO_9205_v114_revD_14Oct2024
English
GridION
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
10/14/2024
ISO_9205_v114_revD_14Oct2024
English
MinION
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
9/30/2024
ISO_9205_v114_revC_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
ISO_9205_v114_revC_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
5/14/2024
ISO_9205_v114_revB_20Mar2024
English
GridION
27th March 2024
- Updated wording for MinKNOW updates to specify v24.02 or newer
5/14/2024
ISO_9205_v114_revB_20Mar2024
English
MinION
27th March 2024
- Updated wording for MinKNOW updates to specify v24.02 or newer
ONT plc is a member of the producer compliance scheme run by ERP UK Ltd, who manage the submission of documentation in support of WEEE compliance for ONT plc’s manufacture and supply of Electrical and Electronic equipment in the UK.