From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
DCS_9187_v114_revJ_12Dec2024
English
MinION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
DCS_9187_v114_revJ_12Dec2024
English
PromethION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/31/2024
DCS_9187_v114_revI_31Jul2024
English
GridION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
7/31/2024
DCS_9187_v114_revI_31Jul2024
English
MinION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
7/31/2024
DCS_9187_v114_revI_31Jul2024
English
PromethION
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
07th March 2024
- Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
- Updated library loading recommendations to 35-50 fmols.
07th March 2024
- Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
- Updated library loading recommendations to 35-50 fmols.
07th March 2024
- Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
- Updated library loading recommendations to 35-50 fmols.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated