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Ligation sequencing V14 - Direct cDNA sequencing (SQK-LSK114) (DCS_9187_v114_revJ_12Dec2024)

DateVersionLanguageDeviceChanges
12/12/2024DCS_9187_v114_revJ_12Dec2024EnglishGridION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024DCS_9187_v114_revJ_12Dec2024EnglishMinION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024DCS_9187_v114_revJ_12Dec2024EnglishPromethION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/31/2024DCS_9187_v114_revI_31Jul2024EnglishGridION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 18th April 2024 - Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
7/31/2024DCS_9187_v114_revI_31Jul2024EnglishMinION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 18th April 2024 - Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
7/31/2024DCS_9187_v114_revI_31Jul2024EnglishPromethION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 18th April 2024 - Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
September 19th, 2023DCS_9187_v114_revD_19Apr2023EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023DCS_9187_v114_revD_19Apr2023EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023DCS_9187_v114_revD_19Apr2023EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
March 12th, 2024DCS_9187_v114_revG_19Apr2023EnglishPromethion Download 07th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step - Updated library loading recommendations to 35-50 fmols.
March 12th, 2024DCS_9187_v114_revG_19Apr2023EnglishGridion Download 07th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step - Updated library loading recommendations to 35-50 fmols.
March 12th, 2024DCS_9187_v114_revG_19Apr2023EnglishMinion Download 07th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step - Updated library loading recommendations to 35-50 fmols.
March 7th, 2024DCS_9187_v114_revF_19Apr2023EnglishGridion Download 06th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
March 7th, 2024DCS_9187_v114_revF_19Apr2023EnglishMinion Download 06th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
March 6th, 2024DCS_9187_v114_revE_19Apr2023EnglishPromethion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
March 6th, 2024DCS_9187_v114_revE_19Apr2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
March 6th, 2024DCS_9187_v114_revE_19Apr2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
August 1st, 2023DCS_9187_v114_revC_19Apr2023EnglishPromethion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023DCS_9187_v114_revC_19Apr2023EnglishGridion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023DCS_9187_v114_revC_19Apr2023EnglishMinion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
April 28th, 2023DCS_9187_v114_revB_19Apr2023EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023DCS_9187_v114_revB_19Apr2023EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023DCS_9187_v114_revB_19Apr2023EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 19th, 2023DCS_9187_v114_revA_19Apr2023EnglishPromethion Download -
April 19th, 2023DCS_9187_v114_revA_19Apr2023EnglishGridion Download -
April 19th, 2023DCS_9187_v114_revA_19Apr2023EnglishMinion Download -

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