11th September 2025
- In 'Overview of the protocol', in the Table under 'Library preparation and sequencing', the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Equipment and consumables', the image of the LSK114 kit in single-use tube format was removed. And instructions for the single-use tube format were removed from section 8, step 2.
- In section 6, 'DNA repair and end-prep', 'Check your flow cell' and section 8, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'DNA repair and end-prep', step 15, instructions were provided on how to resuspend the AMPure XP bead pellet with nuclease-free water.
- In section 8, 'Priming and loading the flow cell', the instruction for removing the flow cell out of the fridge was moved to the top of this section.
- In 'Troubleshooting', section 14, the video links for priming and loading a MinION Flow Cell were replaced with links for the PromethION Flow Cell.
8/12/2025
HVB_9206_v114_revI_12Aug2025
English
PromethION
12 August 2025
- Corrected TE buffer formulation to (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) in the "Size selection of gDNA using SFE Buffer" section of the protocol. Previously said 0.1 mM EDTA.
5/19/2025
HVB_9206_v114_revH_22Jan2025
English
PromethION
22 January 2025
- Document title change
- Extraction: Minor adjustments made to mitigate steps where errors may occur.
- Size selection using SFE: Updated method in-line with latest recommendations.
- Megaruptor shearing: Added more context for handling the device effectively.
- Flow cell wash and reload: updated data images and included image for sequencing read length profile.
1/22/2025
HVB_9206_v114_revH_22Jan2025
English
PromethION
22 January 2025
- Document title change
- Extraction: Minor adjustments made to mitigate steps where errors may occur.
- Size selection using SFE: Updated method in-line with latest recommendations.
- Megaruptor shearing: Added more context for handling the device effectively.
- Flow cell wash and reload: updated data images and included image for sequencing read length profile.
9/30/2024
HVB_9206_v114_revG_14May2024
English
PromethION
21st August 2024
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
8/21/2024
HVB_9206_v114_revG_14May2024
English
PromethION
21st August 2024
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
26 June 2024
- Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol.
26 June 2024
- Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol.
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