Ligation Sequencing gDNA V14 — human variation from blood samples on PromethION (SQK-LSK114)

DateVersion numberLanguageDeviceChanges
Aug 21 2024HVB_9206_v114_revG_14May2024EnglishPromethION
21st August 2024 - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Data acquisition and basecalling instructions updated.
August 21st, 2024HVB_9206_v114_revF_31Jul2024English Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 26 June 2024 - Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol.
July 31st, 2024HVB_9206_v114_revE_14May2024English Download 26 June 2024 - Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol.
June 26th, 2024HVB_9206_v114_revD_14May2024English Download 30 May 2024 - Corrected typo in introduction section.
May 30th, 2024HVB_9206_v114_revC_14May2024English Download 28 May 2024 - Corrected typo in DNA repair and end-prep for incubation following addition of NEBNext FFPE DNA reagents.
May 28th, 2024HVB_9206_v114_revB_14May2024English Download
May 22nd, 2024HVB_9206_v114_revA_14May2024English Download