12 August 2025
- Corrected TE buffer formulation to (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) in the "Size selection of gDNA using SFE Buffer" section of the protocol. Previously said 0.1 mM EDTA.
5/19/2025
HVB_9206_v114_revH_22Jan2025
English
PromethION
22 January 2025
- Document title change
- Extraction: Minor adjustments made to mitigate steps where errors may occur.
- Size selection using SFE: Updated method in-line with latest recommendations.
- Megaruptor shearing: Added more context for handling the device effectively.
- Flow cell wash and reload: updated data images and included image for sequencing read length profile.
1/22/2025
HVB_9206_v114_revH_22Jan2025
English
PromethION
22 January 2025
- Document title change
- Extraction: Minor adjustments made to mitigate steps where errors may occur.
- Size selection using SFE: Updated method in-line with latest recommendations.
- Megaruptor shearing: Added more context for handling the device effectively.
- Flow cell wash and reload: updated data images and included image for sequencing read length profile.
9/30/2024
HVB_9206_v114_revG_14May2024
English
PromethION
21st August 2024
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
8/21/2024
HVB_9206_v114_revG_14May2024
English
PromethION
21st August 2024
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
26 June 2024
- Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol.
26 June 2024
- Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol.
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