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Adeno-associated virus (AAV) sequencing from recombinant adeno-associated virus (rAAV) vectors using SQK-NBD114.24 (AAV_9194_v114_revI_02Jul2025)

DateVersionLanguageDeviceChanges
7/2/2025AAV_9194_v114_revI_02Jul2025EnglishGridION
02nd July 2025 - Increased the timing for priming and loading the flow cell from 5 min to 10 min in table and figure in 'Experiment workflow'. - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - - In section 9, 'Priming and loading the MinION and GridION Flow Cell', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
7/2/2025AAV_9194_v114_revI_02Jul2025EnglishMinION
02nd July 2025 - Increased the timing for priming and loading the flow cell from 5 min to 10 min in table and figure in 'Experiment workflow'. - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - - In section 9, 'Priming and loading the MinION and GridION Flow Cell', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
1/28/2025AAV_9194_v114_revG_13Dec2024EnglishGridION
28th January 2025 In "DNA extraction from rAAV", corrected step 9 to say "Transfer 200 µl of a DNAse treated rAAV sample to a tube containing Proteinase K and repeat for each sample into separate tubes."
1/28/2025AAV_9194_v114_revG_13Dec2024EnglishMinION
28th January 2025 In "DNA extraction from rAAV", corrected step 9 to say "Transfer 200 µl of a DNAse treated rAAV sample to a tube containing Proteinase K and repeat for each sample into separate tubes."
12/13/2024AAV_9194_v114_revF_13Dec2024EnglishGridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Addition of links to user manuals for MinION Mk1B and MinION Mk1D in "Data acquisition and basecalling", "How to start sequencing". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024AAV_9194_v114_revF_13Dec2024EnglishMinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Addition of links to user manuals for MinION Mk1B and MinION Mk1D in "Data acquisition and basecalling", "How to start sequencing". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024AAV_9194_v114_revE_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/15/2024AAV_9194_v114_revE_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
9/30/2024AAV_9194_v114_revD_30Sep2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024AAV_9194_v114_revD_30Sep2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024AAV_9194_v114_revD_20Sep2023EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024AAV_9194_v114_revD_20Sep2023EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
4/23/2024AAV_9194_v114_revC_20Sep2023EnglishGridION
23 April 2024 - Removed "RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019)" from list of Equipment and Consumables as not used in this method. This was an artefact from a previous iteration of the protocol.
4/23/2024AAV_9194_v114_revC_20Sep2023EnglishMinION
23 April 2024 - Removed "RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen, 10777019)" from list of Equipment and Consumables as not used in this method. This was an artefact from a previous iteration of the protocol.
April 23rd, 2024AAV_9194_v114_revB_20Sep2023EnglishGridion Download 07 Feb 2024 - Updated Flow cell wash method in line with updated Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) protocol. - Updated links in protocol - Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between. - Updated method with more extensive instructions on use and handling.
April 23rd, 2024AAV_9194_v114_revB_20Sep2023EnglishMinion Download 07 Feb 2024 - Updated Flow cell wash method in line with updated Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) protocol. - Updated links in protocol - Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between. - Updated method with more extensive instructions on use and handling.
February 7th, 2024AAV_9194_v114_revA_20Sep2023EnglishGridion Download -
February 7th, 2024AAV_9194_v114_revA_20Sep2023EnglishMinion Download -

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