Ligation sequencing amplicons V14 (SQK-LSK114) (ACDE_9163_v114_revX_09May2025)

DateVersionLanguageDeviceChanges
5/14/2025ACDE_9163_v114_revX_09May2025EnglishFlongle
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/14/2025ACDE_9163_v114_revX_09May2025EnglishGridION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/14/2025ACDE_9163_v114_revX_09May2025EnglishMinION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/14/2025ACDE_9163_v114_revX_09May2025EnglishPromethION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/12/2025ACDE_9163_v114_revX_09May2025EnglishFlongle
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/12/2025ACDE_9163_v114_revX_09May2025EnglishGridION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/12/2025ACDE_9163_v114_revX_09May2025EnglishMinION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/12/2025ACDE_9163_v114_revX_09May2025EnglishPromethION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025ACDE_9163_v114_revX_09May2025EnglishFlongle
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025ACDE_9163_v114_revX_09May2025EnglishGridION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025ACDE_9163_v114_revX_09May2025EnglishMinION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025ACDE_9163_v114_revX_09May2025EnglishPromethION
9th May 2025 - In 'Overview', library preparation time was increased to ~75 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 3 'End-prep', step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
1/30/2025ACDE_9163_v114_revW_30Jan2025EnglishFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025ACDE_9163_v114_revW_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025ACDE_9163_v114_revW_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025ACDE_9163_v114_revW_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024ACDE_9163_v114_revV_12Dec2024EnglishFlongle
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024ACDE_9163_v114_revV_12Dec2024EnglishGridION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024ACDE_9163_v114_revV_12Dec2024EnglishMinION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024ACDE_9163_v114_revV_12Dec2024EnglishPromethION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024ACDE_9163_v114_revU_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024ACDE_9163_v114_revU_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024ACDE_9163_v114_revU_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024ACDE_9163_v114_revU_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
August 21st, 2024ACDE_9163_v114_revT_30Jul2024EnglishFlongle Download 08 Aug 2024 Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents "Lambda DNA (LMD) fragment length is 48.5 kb." 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024ACDE_9163_v114_revT_30Jul2024EnglishMinion Download 08 Aug 2024 Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents "Lambda DNA (LMD) fragment length is 48.5 kb." 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024ACDE_9163_v114_revT_30Jul2024EnglishPromethion Download 08 Aug 2024 Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents "Lambda DNA (LMD) fragment length is 48.5 kb." 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024ACDE_9163_v114_revT_30Jul2024EnglishGridion Download 08 Aug 2024 Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents "Lambda DNA (LMD) fragment length is 48.5 kb." 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 30th, 2024ACDE_9163_v114_revS_29Jun2022EnglishFlongle Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 30th, 2024ACDE_9163_v114_revS_29Jun2022EnglishMinion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 30th, 2024ACDE_9163_v114_revS_29Jun2022EnglishPromethion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 30th, 2024ACDE_9163_v114_revS_29Jun2022EnglishGridion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
March 7th, 2024ACDE_9163_v114_revR_29Jun2022EnglishFlongle Download 2nd January 2024 - Updated the protocol to a slimmed down template.
March 7th, 2024ACDE_9163_v114_revR_29Jun2022EnglishMinion Download 2nd January 2024 - Updated the protocol to a slimmed down template.
March 7th, 2024ACDE_9163_v114_revR_29Jun2022EnglishPromethion Download 2nd January 2024 - Updated the protocol to a slimmed down template.
March 7th, 2024ACDE_9163_v114_revR_29Jun2022EnglishGridion Download 2nd January 2024 - Updated the protocol to a slimmed down template.
January 2nd, 2024ACDE_9163_v114_revQ_29Jun2022EnglishFlongle Download 19th August 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for amplicon DNA - Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
January 2nd, 2024ACDE_9163_v114_revQ_29Jun2022EnglishMinion Download 19th August 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for amplicon DNA - Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
January 2nd, 2024ACDE_9163_v114_revQ_29Jun2022EnglishPromethion Download 19th August 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for amplicon DNA - Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
January 2nd, 2024ACDE_9163_v114_revQ_29Jun2022EnglishGridion Download 19th August 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for amplicon DNA - Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
September 19th, 2023ACDE_9163_v114_revO_29Jun2022EnglishFlongle Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023ACDE_9163_v114_revO_29Jun2022EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023ACDE_9163_v114_revO_29Jun2022EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023ACDE_9163_v114_revO_29Jun2022EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
August 1st, 2023ACDE_9163_v114_revN_29Jun2022EnglishFlongle Download May 2023 - This kit has moved to released phase.
August 1st, 2023ACDE_9163_v114_revN_29Jun2022EnglishMinion Download May 2023 - This kit has moved to released phase.
August 1st, 2023ACDE_9163_v114_revN_29Jun2022EnglishPromethion Download May 2023 - This kit has moved to released phase.
August 1st, 2023ACDE_9163_v114_revN_29Jun2022EnglishGridion Download May 2023 - This kit has moved to released phase.
April 28th, 2023ACDE_9163_v114_revM_29Jun2022EnglishFlongle Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023ACDE_9163_v114_revM_29Jun2022EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023ACDE_9163_v114_revM_29Jun2022EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023ACDE_9163_v114_revM_29Jun2022EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
March 24th, 2023ACDE_9163_v114_revL_29Jun2022English- Download 24 March 2023: - Updated amplicon sample DNA input quantities to fmol
March 15th, 2023ACDE_9163_v114_revK_29Jun2022EnglishFlongle Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023ACDE_9163_v114_revK_29Jun2022EnglishMinion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023ACDE_9163_v114_revK_29Jun2022EnglishPromethion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023ACDE_9163_v114_revK_29Jun2022EnglishGridion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
January 6th, 2023ACDE_9163_v114_revJ_29Jun2022EnglishFlongle Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023ACDE_9163_v114_revJ_29Jun2022EnglishMinion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023ACDE_9163_v114_revJ_29Jun2022EnglishPromethion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023ACDE_9163_v114_revJ_29Jun2022EnglishGridion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
November 30th, 2022ACDE_9163_v114_revI_29Jun2022EnglishFlongle Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
November 30th, 2022ACDE_9163_v114_revI_29Jun2022EnglishMinion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
November 30th, 2022ACDE_9163_v114_revI_29Jun2022EnglishPromethion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
November 30th, 2022ACDE_9163_v114_revI_29Jun2022EnglishGridion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
October 27th, 2022ACDE_9163_v114_revH_29Jun2022EnglishFlongle Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input. - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022ACDE_9163_v114_revH_29Jun2022EnglishMinion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input. - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022ACDE_9163_v114_revH_29Jun2022EnglishPromethion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input. - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022ACDE_9163_v114_revH_29Jun2022EnglishGridion Download 27th October 2022 - Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input. - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 26th, 2022ACDE_9163_v114_revG_29Jun2022EnglishFlongle Download 20th October 2022 - Added Flongle branch
October 26th, 2022ACDE_9163_v114_revG_29Jun2022EnglishMinion Download 20th October 2022 - Added Flongle branch
October 26th, 2022ACDE_9163_v114_revG_29Jun2022EnglishPromethion Download 20th October 2022 - Added Flongle branch
October 26th, 2022ACDE_9163_v114_revG_29Jun2022EnglishGridion Download 20th October 2022 - Added Flongle branch
October 4th, 2022ACDE_9163_v114_revF_29Jun2022EnglishMinion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022ACDE_9163_v114_revF_29Jun2022EnglishPromethion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022ACDE_9163_v114_revF_29Jun2022EnglishGridion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
August 18th, 2022ACDE_9163_v114_revE_29Jun2022EnglishMinion Download 18th August 2022 - Updated kit contents image
August 18th, 2022ACDE_9163_v114_revE_29Jun2022EnglishPromethion Download 18th August 2022 - Updated kit contents image
August 18th, 2022ACDE_9163_v114_revE_29Jun2022EnglishGridion Download 18th August 2022 - Updated kit contents image
August 10th, 2022ACDE_9163_v114_revD_29Jun2022EnglishMinion Download 10th August 2022 Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022ACDE_9163_v114_revD_29Jun2022EnglishPromethion Download 10th August 2022 Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022ACDE_9163_v114_revD_29Jun2022EnglishGridion Download 10th August 2022 Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022ACDE_9163_v114_revC_29Jun2022EnglishGridion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022ACDE_9163_v114_revC_29Jun2022EnglishMinion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022ACDE_9163_v114_revC_29Jun2022EnglishPromethion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
July 21st, 2022ACDE_9163_v114_revB_29Jun2022EnglishMinion Download 21st July 2022 - Updated the flow cell loading recommendations for MinION/GridION and PromethION flow cells
July 21st, 2022ACDE_9163_v114_revB_29Jun2022EnglishPromethion Download 21st July 2022 - Updated the flow cell loading recommendations for MinION/GridION and PromethION flow cells
July 21st, 2022ACDE_9163_v114_revB_29Jun2022EnglishGridion Download 21st July 2022 - Updated the flow cell loading recommendations for MinION/GridION and PromethION flow cells
June 30th, 2022ACDE_9163_v114_revA_29Jun2022EnglishMinion Download -
June 30th, 2022ACDE_9163_v114_revA_29Jun2022EnglishPromethion Download -
June 30th, 2022ACDE_9163_v114_revA_29Jun2022EnglishGridion Download -
June 29th, 2022ACDE_LSK114_v1EnglishMinion Download -
June 29th, 2022ACDE_LSK114_v1EnglishPromethion Download -
June 29th, 2022ACDE_LSK114_v1EnglishGridion Download -
June 29th, 2022ACDE_LSK114_v1EnglishMinion Download -
June 29th, 2022ACDE_LSK114_v1EnglishPromethion Download -
June 29th, 2022ACDE_LSK114_v1EnglishGridion Download -