02 July 2025
- Updated protocol introduction section to condensed format.
- Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
- Added P2i as a compatible device in 'Compatibility of this protocol'.
- Updated information for new kit format with increased Short Fragment Buffer (SFB) volume.
- Updated wash steps in the native barcode ligation section of the protocol to use SFB instead of 80% Ethanol.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
7/2/2025
NBA_9168_v114_revS_02Jul2025
English
MinION
02 July 2025
- Updated protocol introduction section to condensed format.
- Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
- Added P2i as a compatible device in 'Compatibility of this protocol'.
- Updated information for new kit format with increased Short Fragment Buffer (SFB) volume.
- Updated wash steps in the native barcode ligation section of the protocol to use SFB instead of 80% Ethanol.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
7/2/2025
NBA_9168_v114_revS_02Jul2025
English
PromethION
02 July 2025
- Updated protocol introduction section to condensed format.
- Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
- Added P2i as a compatible device in 'Compatibility of this protocol'.
- Updated information for new kit format with increased Short Fragment Buffer (SFB) volume.
- Updated wash steps in the native barcode ligation section of the protocol to use SFB instead of 80% Ethanol.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
1/30/2025
NBA_9168_v114_revR_30Jan2025
English
GridION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
NBA_9168_v114_revR_30Jan2025
English
MinION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
NBA_9168_v114_revR_30Jan2025
English
PromethION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024
NBA_9168_v114_revQ_12Dec2024
English
GridION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9168_v114_revQ_12Dec2024
English
MinION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9168_v114_revQ_12Dec2024
English
PromethION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024
NBA_9168_v114_revP_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/15/2024
NBA_9168_v114_revP_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/15/2024
NBA_9168_v114_revP_20Nov2024
English
PromethION
November 2024
- Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
8/21/2024
NBA_9168_v114_revO_15Sep2022
English
GridION
21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
8/21/2024
NBA_9168_v114_revO_15Sep2022
English
MinION
21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
8/21/2024
NBA_9168_v114_revO_15Sep2022
English
PromethION
21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
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