Direct RNA sequencing (SQK-RNA004)

DateVersion numberLanguageDeviceChanges
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EspañolGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EspañolMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EspañolPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EspañolGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EspañolMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EspañolPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 16 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 14 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 14 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 14 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 14 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 14 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 14 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Oct 11 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Sep 23 2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Sep 23 2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Sep 23 2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Sep 23 2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Sep 23 2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Sep 23 2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
Aug 27 2024DRS_9195_v4_revD_20Sep2023EnglishGridION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
Aug 27 2024DRS_9195_v4_revD_20Sep2023EnglishMinION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
Aug 27 2024DRS_9195_v4_revD_20Sep2023EnglishPromethION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
Aug 27 2024DRS_9195_v4_revD_20Sep2023中文GridION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
Aug 27 2024DRS_9195_v4_revD_20Sep2023中文MinION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
Aug 27 2024DRS_9195_v4_revD_20Sep2023中文PromethION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
August 12th, 2024DRS_9195_v4_revC_20Sep2023EnglishPromethion Download 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
August 12th, 2024DRS_9195_v4_revC_20Sep2023EnglishGridion Download 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
August 12th, 2024DRS_9195_v4_revC_20Sep2023EnglishMinion Download 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
November 15th, 2023DRS_9195_v4_revB_20Sep2023EnglishPromethion Download
November 15th, 2023DRS_9195_v4_revB_20Sep2023EnglishGridion Download
November 15th, 2023DRS_9195_v4_revB_20Sep2023EnglishMinion Download