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Direct RNA sequencing XL (SQK-RNA004-XL) (DRS_9219_v4_revE_13Nov2025)

DateVersionLanguageDeviceChanges
11/13/2025DRS_9219_v4_revE_13Nov2025EnglishGridION
13th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array.
11/13/2025DRS_9219_v4_revE_13Nov2025EnglishMinION
13th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array.
11/13/2025DRS_9219_v4_revE_13Nov2025EnglishPromethION
13th November 2025 - In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array.
8/29/2025DRS_9219_v4_revD_29Aug2025EnglishGridION
29th August 2025 - In 'Compatibility of this protocol', under the PromethION branch, the PromethION 2 Integrated was included as a compatible device and the link to its user manual was added to 'Data acquisition and basecalling'.
8/29/2025DRS_9219_v4_revD_29Aug2025EnglishMinION
29th August 2025 - In 'Compatibility of this protocol', under the PromethION branch, the PromethION 2 Integrated was included as a compatible device and the link to its user manual was added to 'Data acquisition and basecalling'.
8/29/2025DRS_9219_v4_revD_29Aug2025EnglishPromethION
29th August 2025 - In 'Compatibility of this protocol', under the PromethION branch, the PromethION 2 Integrated was included as a compatible device and the link to its user manual was added to 'Data acquisition and basecalling'.
7/16/2025DRS_9219_v4_revC_16Jul2025EnglishGridION
16th July 2025 - In 'Data acquisition and Basecalling', 'To start a sequencing run on MinKNOW', the script for the standard RNA004 was replaced with the script for RNA004-XL in step 3, as a result of the update to MinKNOW.
7/16/2025DRS_9219_v4_revC_16Jul2025EnglishMinION
16th July 2025 - In 'Data acquisition and Basecalling', 'To start a sequencing run on MinKNOW', the script for the standard RNA004 was replaced with the script for RNA004-XL in step 3, as a result of the update to MinKNOW.
7/16/2025DRS_9219_v4_revC_16Jul2025EnglishPromethION
16th July 2025 - In 'Data acquisition and Basecalling', 'To start a sequencing run on MinKNOW', the script for the standard RNA004 was replaced with the script for RNA004-XL in step 3, as a result of the update to MinKNOW.
5/19/2025DRS_9219_v4_revB_19May2025EnglishGridION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishMinION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishPromethION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishGridION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishMinION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishPromethION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
2/19/2025DRS_9219_v4_revA_19Feb2025EnglishGridION
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2/19/2025DRS_9219_v4_revA_19Feb2025EnglishMinION
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2/19/2025DRS_9219_v4_revA_19Feb2025EnglishPromethION
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