08 Aug 2024
- Rephrased sentence to include LMD fragment length in Appendix/Kit reagents/Sequencing kit reagents/ Lambda DNA (LMD)
"The Lambda DNA (LMD) is a pure, high-quality 48.5 kb DNA fragment that can be used..."
25 April 2024
- Clarified the distinction RLB012 and RLB012A sequences in the 'Rapid barcode primers' within the 'Barcode sequences' section of the 'Appendix'.
25 April 2024
- Clarified the distinction RLB012 and RLB012A sequences in the 'Rapid barcode primers' within the 'Barcode sequences' section of the 'Appendix'.
6th December 2023
- Updated the format of the document to improve useability, including the addition of an available kits and legacy kits section.
- Adapter and barcode sequences have been moved to the back of the document, into the appendix
- Newly released kits have been added to the document.
6th December 2023
- Updated the format of the document to improve useability, including the addition of an available kits and legacy kits section.
- Adapter and barcode sequences have been moved to the back of the document, into the appendix
- Newly released kits have been added to the document.
April 2023
- Correct the SQK-PCB111.24 adapter sequence for CRTA in the "Barcoding and the available kits" section. This is now consistent with the adapter sequence in the SQK-PCS111 kit
December 2022
- Added the adapter sequences for kits in the 'Ligation Sequencing Kit family', Rapid Sequencing Kit family', 'Barcoding and the available kits' and the 'RNA and cDNA sequencing and kits' pages
2nd March 2022
Updated the "Optional size selection" section of the "Sample input and recommendations" chapter to the updated data using the "size selection buffer" method.
2nd December 2021
- Updated to include new kit releases:
1. Ligation Sequencing kits (SQK-LSK112 and SQK-LSK112-XL)
2. Native Barcoding Kits (SQK-NBD112.24, SQK-NBD112.96 and EXP-NBD112)
3. Flow Cell Wash Kit XL (EXP-WSH004-XL)
4. PCR-cDNA Sequencing Kit (SQK-PCS111)
5. PCR-cDNA Barcoding Kit (SQK-PCB111.24)
- Removed the output star rating system on all kits
- In the "Available kits", the "Ligation Sequencing Kit family" has been updated to include the Ligation Sequencing kits.
- In the "Barcoding and the available kits", Native Barcoding kits have been added.
- In the "RNA and cDNA sequencing and kits", the PCR-cDNA Sequencing Kit and PCR-cDNA Barcoding Kit have been added.
15th July 2021
- In the 'Available kits' section, the 'Barcoding and the available kits: Rapid chemistry-based PCR barcoding kits' section has been updated to include the Midnight RT PCR Expansion (EXP-MRT001) and Spike Seq RT PCR Expansion (EXP-SRT001) and removed a paragraph below the list of rapid chemistry-based PCR barcoding kits.
8th July 2021
- In the 'Making the most of your flow cell' section, the 'Flow cell wash' snippet has been updated with an updated image of pore states observed on a flow cell before and after wash steps have been performed.
17th June 2021
- Document has been re-formatted and updated with more information on out kit 10 upgrade to the ligation sequencing kit.
- All kits now have information for use cases, recommended input, library workflow and expected output using out star rating system.
- Added sections include 'Sample input recommendations' and 'Making the most of your flow cell'
26th March 2021
- Added an 'Ultra-Long DNA Sequencing Kit (SQK-ULK001)' snippet to the 'Rapid Sequencing Kit Family' in the 'Available kits' section outlining the workflow of the kit
- Updated 'Barcoding kits' in the 'Available kits' section to include 'Rapid chemistry-based barcoding kits' which cover information regarding the Rapid Barcoding kit (SQK-RBK004) and the Rapid Barcoding Kit 96 (SQK-RBK110.96).
- Added 'Adapter Mix II Expansion (EXP-AMII001) to the 'Barcoding kits'.
- Updated 'Ultra-long reads' in the 'Applications' section.