27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
23rd January 2020
Replaced DNA input recommendations for R10 flow cells with R10.3:
- starting with 1.5-3 µg (or 150-300 fmol) DNA
- loading 25-75 fmols onto the flow cell
23rd January 2020
Replaced DNA input recommendations for R10 flow cells with R10.3:
- starting with 1.5-3 µg (or 150-300 fmol) DNA
- loading 25-75 fmols onto the flow cell
20th September 2019
- A note has been added at the end of the library preparation instructions about using the Sequencing Auxiliary Vials (EXP-AUX001) for splitting a library into two aliquots
20th September 2019
- A note has been added at the end of the library preparation instructions about using the Sequencing Auxiliary Vials (EXP-AUX001) for splitting a library into two aliquots
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
19th July 2019
- Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
- The cDNA option has been removed, and added to the Direct cDNA Sequencing Kit instructions instead.
Updated with new information for MinKNOW release v19.06:
- Compression of queued reads
- Pause functionality
- UI improvements including:
- Flow cell health at platform QC
- Flow cell status on landing page
- R10 flow cell running scripts
- New Fast Models for RNA and DNA
- PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files
- Flongle hardware check for confirming Flongle adapter functions correctly
Update to the flow cell priming section, and instructions for topping up the flow cell with fuel, following the release of the Flow Cell Priming Kit (EXP-FLP002)
Updates to kit contents with the introduction of the Control Expansion kit (EXP-CTL001) and removal of the Lambda Control DNA from the Ligation Sequencing Kit