Fusion transcripts
Full-length sequencing of fusion transcripts
The accurate characterisation of fusion transcripts is of high importance for clinical research into diseases, including some forms of cancer. Confident identification of fusion transcripts requires sequencing reads to span the fusion junction and include sufficient sequence on either side for accurate identification.
This capacity is often limited when using legacy short-read sequencing approaches. However, with Oxford Nanopore reads of unrestricted length, fusion transcripts can be sequenced end to end in single reads, enabling comprehensive characterisation of fusions and their precise splice junctions.
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Confident fusion transcript characterisation with full-length reads
In this application note, discover how the latest Oxford Nanopore cDNA-PCR sequencing protocol improved transcript coverage and generated longer reads compared with the previous protocol and alternative RNA sequencing methods.
The value of full-length transcripts without bias
This white paper introduces the facility of RNA and cDNA nanopore sequencing to deliver full-length transcript information, isoform characterisation and quantification, and RNA virus identification.
Recommended device for fusion transcript sequencing
MinION
Delivering real-time sequencing anywhere, the portable MinION enables on-demand sequencing of full-length fusion transcripts. Targeted fusion or whole-transcriptome sequencing approaches can be utilised, depending on the goals of an experiment.
