RNA sequencing with nanopore technology
New: nanopore for RNA sequencing. Sequence complete RNA strands directly and in real-time. Or, cDNA ‘strand-switching’ protocol also allows full-length sequencing of cDNA, either with or without PCR amplification.
- Long read lengths
for full-length transcripts - Real-time RNA
sequencing analysis - PCR-free protocol
to reduce bias - Explore epigenetic modifications by directly sequencing the RNA
- Simple and scalable
How are your peers using Nanopore RNA sequencing?
Which approach is best for you?
Direct RNA sequencing
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PCR cDNA
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PCR-free cDNA
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Prepare
- Simple protocol kit for direct RNA sequencing
- 500 ng RNA (polyA tailed)
- Less than 2 hours prep time
- No cDNA required
Sequence
- Sequence on MinION, GridION or PromethION
- Real-time sequencing -
stop at any time
Analyse
- Up to 1 million full-length reads
- Modified base detection via open source tools
- Full-length RNA sequences without reverse transcriptase or PCR bias
- Analyse using the device or local infrastructure basecalling
- Real-time analysis
Prepare
- Simple protocol with rapid attachment primers
- 50 ng RNA (polyA tailed)
- ~2 hours prep time
Sequence
- Sequence on MinION, GridION or PromethION
- Real-time sequencing -
stop at any time - Multiplexing options available
Analyse
- 6-10 million full-length reads
- Quanitification and identification of full-length transcripts
- Analysis using open source tools (e.g. BWA)
- Analyse using the device or local infrastructure basecalling
- Real-time analysis
Prepare
- Simple protocol kit for cDNA preparation
- 250 ng RNA (polyA tailed)
- ~3.5 hours prep time
- PCR-free protocol
Sequence
- Sequence on MinION, GridION or PromethION
- Real-time sequencing -
stop at any time
Analyse
- 1 million full-length reads
- Quanitifiable full-length transcripts without PCR bias
- Analysis using open source tools (e.g. BWA)
- Analyse using the device or local infrastructure basecalling
- Real-time analysis