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My library isn't going down the SpotOn port, why?
My library isn't going down the SpotOn port, why?
For an in-depth explanation and demonstration of flow cell loading, please refer to this video from the Lesson Library.
If the library will not enter the Spot-ON port, please collect the library that has not entered the port, add another 200 uL of priming mix to the priming port, and load the library again.
If this does not work for you, you can try the following:
Close the priming port cover and the Spot-ON port cover.
Slowly withdraw all liquid from one of the waste ports. If there are visible precipitants, you can add and withdraw 1 mL of warm water to the waste area to dissolve any blockages.
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As a last resort, If the problem persists you can try the following.
Add 15-20 uL of priming buffer on top of the blocked Spot-ON port.
Drawback 15 uL from the priming port and the buffer should get sucked into the port.
If none of the above solutions work for you, please contact the support team via Live Chat on our website.
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