Evaluation of Oxford Nanopore whole-genome methylation sequencing for phenotype inference and association testing
:quality(80))
- Published on:
:quality(80))
This poster evaluates Oxford Nanopore whole-genome methylation sequencing (WGMS) across three common methylation applications: epigenetic age prediction ('epigenetic clocks'), whole-blood cell-type deconvolution, and a proof-of-principle epigenome-wide association study (EWAS). Using healthy whole-blood samples with matched EPIC array data, we show how WGMS compares with arrays while reaching CpG sites that arrays do not target.
Download this poster to discover:
- How WGMS methylation estimates closely match those from EPIC v2 arrays at shared CpG sites
- How WGMS performed comparably to arrays for epigenetic age prediction, cell-type deconvolution, and EWAS
- Why these results suggest WGMS can serve as a direct replacement for array-based methylation analyses
- How WGMS extends coverage to the large majority of CpGs that arrays miss
- How sequence and haplotype context is added alongside methylation data
